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CD31/PECAM-1 Antibody (9G11) [Biotin]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow
Clone
9G11
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin
Concentration
LYOPH

CD31/PECAM-1 Antibody (9G11) [Biotin] Summary

Immunogen
Activated HUVEC human umbilical vein endothelial cells
Specificity
Detects human CD31/PECAM‑1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human
(rh) E‑Selectin, rhICAM-1, -2, -3, rhVCAM-1, or recombinant mouse VCAM-1 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PECAM1
Purity Statement
Protein A or G purified from ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL
Readout System
Publications
Read Publications using
BAM3567 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD31/PECAM-1 Antibody (9G11) [Biotin]

  • adhesion molecule
  • CD31 antigen
  • CD31
  • CD31/EndoCAM
  • EndoCAM
  • FLJ34100
  • FLJ58394
  • GPIIA'
  • PECA1
  • PECAM1
  • PECAM-1
  • PECAM-1, CD31/EndoCAM
  • platelet endothelial cell adhesion molecule
  • platelet endothelial cell adhesion molecule-1
  • platelet/endothelial cell adhesion molecule

Background

The CD31 adhesion molecule, also known as PECAM-1, is expressed in large amounts on endothelial cells at intercellular junctions and on T cell subsets, and to a lesser extent on platelets and most other leukocytes such as monocytes and neutrophils. CD31 binds to itself homotypically, and also to the leukocyte integrin alpha v beta 3 heterotypically. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells. CD31 has been found in human plasma, and the presence of this circulating isoform is suggested to modulate the transendothelial migration of leukocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD31/PECAM-1 Antibody (BAM3567)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: Bioassay, Cell Purification, Cell Selection, Flow Cytometry.


Filter By Application
Bioassay
(1)
Cell Purification
(1)
Cell Selection
(1)
Flow Cytometry
(1)
All Applications
Filter By Species
Human
(4)
All Species
Showing Publications 1 - 4 of 4.
Publications using BAM3567 Applications Species
R Nakato, Y Wada, R Nakaki, G Nagae, Y Katou, S Tsutsumi, N Nakajima, H Fukuhara, A Iguchi, T Kohro, Y Kanki, Y Saito, M Kobayashi, A Izumi-Tagu, N Osato, K Tatsuno, A Kamio, Y Hayashi-Ta, H Wada, S Ohta, M Aikawa, H Nakajima, M Nakamura, RC McGee, KW Heppner, T Kawakatsu, M Genno, H Yanase, H Kume, T Senbonmats, Y Homma, S Nishimura, T Mitsuyama, H Aburatani, H Kimura, K Shirahige Comprehensive epigenome characterization reveals diverse transcriptional regulation across human vascular endothelial cells Epigenetics Chromatin, 2019-12-19;12(1):77. 2019-12-19 [PMID: 31856914] (Cell Purification, Human) Cell Purification Human
I Skachkov, Y Luan, ST van Tiel, AFW van der St, N de Jong, MR Bernsen, K Kooiman SPIO labeling of endothelial cells using ultrasound and targeted microbubbles at diagnostic pressures PLoS ONE, 2018-09-20;13(9):e0204354. 2018-09-20 [PMID: 30235336] (Bioassay, Human) Bioassay Human
Chen K, Ding A, Ding Y, Ghanekar A High-throughput flow cytometry screening of human hepatocellular carcinoma reveals CD146 to be a novel marker of tumor-initiating cells. Biochem Biophys Rep, 2016-08-12;8(0):107-113. 2016-08-12 [PMID: 28955945] (Flow Cytometry, Human) Flow Cytometry Human
Brownell J, Wagoner J, Lovelace E, Thirstrup D, Mohar I, Smith W, Giugliano S, Li K, Crispe I, Rosen H, Polyak S Independent, parallel pathways to CXCL10 induction in HCV-infected hepatocytes. J Hepatol, 2013-06-12;59(4):701-8. 2013-06-12 [PMID: 23770038] (Cell Selection, Human) Cell Selection Human

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional CD31/PECAM-1 Products

Blogs on CD31/PECAM-1.


  Read full blog post.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

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Bioinformatics

Gene Symbol PECAM1