![Western Blot: CD3 epsilon Antibody (C3e/1931) - Azide and BSA Free [NBP2-79840] - Western Blot Analysis of human Jurkat cell lysate using CD3 epsilon Antibody (C3e/1931).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-C3e-1931-Azide-and-BSA-Free-Western-Blot-NBP2-79840-img0002.jpg "Western Blot: CD3 epsilon Antibody (C3e/1931) - Azide and BSA Free [NBP2-79840] - Western Blot Analysis of human Jurkat cell lysate using CD3 epsilon Antibody (C3e/1931).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, IHC, MA, CyTOF-ready |
| Clone | C3e/1931 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79706). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | A recombinant human CD3 epsilon protein fragment (around aa 23-119) (exact sequence is proprietary) (Uniprot: P07766) |
| Localization | Cell Surface, Cytoplasm |
| Marker | T-Cell Marker |
| Specificity | Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CD3E |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA. Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
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| Theoretical MW | 20 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
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| Publication using NBP2-79840 | Applications | Species |
|---|---|---|
| Yu SC, Chen KC, Huang RY Nodal reactive proliferation of monocytoid B-cells may represent atypical memory B-cells Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi 2023-04-11 [PMID: 37080839] (IHC-P, Human) | IHC-P | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for CD3 epsilon Antibody (NBP2-79840)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CD3E |
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