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TCR gamma/delta Antibody (GL-3) [Unconjugated]

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Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC‑conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Hamster Anti-Mouse TCR gamma /δ Monoclonal Antibody (Catalog # MAB7297) or (B) ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, CyTOF-ready
Clone
GL-3
Clonality
Monoclonal
Host
Hamster
Conjugate
Unconjugated

Order Details

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TCR gamma/delta Antibody (GL-3) [Unconjugated] Summary

Immunogen
Mouse intraepithelial lymphocytes
Specificity
Detects mouse TCR gamma /δ.
Source
N/A
Isotype
IgG2 Kappa
Clonality
Monoclonal
Host
Hamster
Gene
Cd3d
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-reported
  • Flow Cytometry 0.25 ug/10^6 cells
Publications
Read Publications using
MAB7297 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TCR gamma/delta Antibody (GL-3) [Unconjugated]

  • delta polypeptide
  • T cell receptor gamma locus
  • T3D
  • T-cell antigen receptor, gamma polypeptide
  • T-cell rearranging gene, gamma
  • T-cell receptor, gamma cluster
  • TCR gamma/delta
  • TCRG
  • TiT3
  • TRG

Background

The gamma δ T-cell receptor (TCR) is a heteromer that includes type I transmembrane CD3 gamma and CD3δ glycoprotein subunits of the Ig superfamily. T gamma δ cells develop as a minor population in the thymus and migrate mainly to in skin and intestinal epithelial layers. Mouse and rat CD3 gamma and CD3δ are synthesized as 182 and 173 amino acid (aa) precursors that result in 160 and 152 aa mature proteins with 94 and 84 aa extracellular domains (ECD), respectively. The germline ECD sequences of CD3 gamma and CD3δ share 71% and 76% aa identity between mouse and rat, respectively, while both proteins and species share 57-62% aa identity with human CD3 gamma and CD3δ. Mouse intraepithelial lymphocytes from the small intestine, which contain a major population of gamma δ T cells, were used as the immunogen for the GL-3/5E11 antibody(1).
  1. Goodman, T. and L. Lefrancois (1989) J. Exp. Med. 170:2401569.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TCR gamma/delta Antibody (MAB7297)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: Flow Cytometry, Neutralization.


Filter By Application
Flow Cytometry
(1)
Neutralization
(1)
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(2)
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Bioinformatics

Gene Symbol Cd3d