![Western Blot: CD3 epsilon Antibody (C3e/1308) [NBP2-53387] - Western Blot Analysis of human Jurkat cell lysate using CD3 epsilon Antibody (C3e/1308).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-C3e-1308-Western-Blot-NBP2-53387-img0005.jpg "Western Blot: CD3 epsilon Antibody (C3e/1308) [NBP2-53387] - Western Blot Analysis of human Jurkat cell lysate using CD3 epsilon Antibody (C3e/1308).")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, IHC, Func |
| Clone | C3e/1308 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54392) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant human CD3 epsilon fragment (aa 23-119) (Uniprot: P07766) |
| Localization | Cell surface and Cytoplasmic |
| Marker | T-Cell Marker |
| Specificity | Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms. |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CD3E |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Use in Multiplex Immunoassay reported in scientific literature (PMID: 31942075). Optimal dilution for a specific application should be determined. Western Blot: 1-2ug/ml for 60 minutes at RT. |
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| Theoretical MW | 20 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
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| Publications using NBP2-53387 | Applications | Species |
|---|---|---|
| Koedijk J, van der Werf I, Vermeulen M et al. Spatial analysis reveals distinct immune phenotypes and tertiary lymphoid structure-like aggregates in pediatric acute myeloid leukemia medRxiv 2023-03-08 Details: Citation using the version of this antibody. |
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| L Xu, J Guo, DG Moledina, LG Cantley Immune-mediated tubule atrophy promotes acute kidney injury to chronic kidney disease transition Nature Communications, 2022-08-19;13(1):4892. 2022-08-19 [PMID: 35986026] | ||
| Mashima K, Oh I, Fujiwara K, et al. Comparison of alemtuzumab, anti-thymocyte globulin, and post-transplant cyclophosphamide for graft-versus-host disease and graft-versus-leukemia in murine models PloS one 2021-01-11 [PMID: 33428661] (IF/IHC, Mouse) | IF/IHC | Mouse |
| Helmink BA, Reddy SM, Gao J et al. B cells and tertiary lymphoid structures promote immunotherapy response Nature. [PMID: 31942075] (MI, Human) | MI | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for CD3 epsilon Antibody (NBP2-53387)Find related products by research area.
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![Flow Cytometry: CD3 epsilon Antibody (C3e/1308) [NBP2-53387] - Flow Cytometric Analysis of Jurkat cells using CD3 epsilon Antibody (C3e/1308) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-C3e-1308-Flow-Cytometry-NBP2-53387-img0004.jpg "Flow Cytometry: CD3 epsilon Antibody (C3e/1308) [NBP2-53387] - Flow Cytometric Analysis of Jurkat cells using CD3 epsilon Antibody (C3e/1308) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).")
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