CD133 Antibodies
We offer CD133 Antibodies for use in common research applications: Flow (Intracellular), Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA. Each CD133 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our CD133 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the CD133 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our CD133 polyclonal antibodies and browse our CD133 monoclonal antibody catalog.
Alternate Names for CD133 Antibodies
anti-CD133 antibody, anti-PROM1 antibody, anti-AC133 Stargardt disease 4 (autosomal dominant) antibody, anti-CD133 retinal 2 antibody, anti-prominin (mouse)-like 1 antibody, anti-prominin 1 antibody
25 Results for "cd133" in Primary Antibodies
![Western Blot: CD133 Antibody - BSA Free [NB120-16518] - Predicted band size : 97 kDa recognizes 97 kDa human CD133 in Y79 cells. Band is at ~120 kDa due to protein glycosylation.](http://images.novusbio.com/fullsize/CD133-Antibody---BSA-Free-Western-Blot-NB120-16518-img0003.jpg "Western Blot: CD133 Antibody - BSA Free [NB120-16518] - Predicted band size : 97 kDa recognizes 97 kDa human CD133 in Y79 cells. Band is at ~120 kDa due to protein glycosylation.")
![Immunocytochemistry/Immunofluorescence: CD133 Antibody - BSA Free [NB120-16518] - Characterization of Scattered tubular-like cells (STCs) isolated from pig kidneys. A) Representative immunofluorescence staining (original magnification 40X) for the STC surface markers CD133 NB120-16518G (red) and CD24 (green) in isolated swine STCs. B) Flow cytometry analysis of isolated STCs showing that 97% of cells expressed CD133, 95% CD24, and 98% were double-positive for CD133 and CD24 (n = 3 each). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31614781/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD133 Antibody - BSA Free-Immunocytochemistry-Immunofluorescence-NB120-16518-img0008.jpg)
![Immunohistochemistry: CD133 Antibody - BSA Free [NB120-16518] - Immunostaining for CD133 (NB120-16518) in normal ovarian and tumor tissue sections. Staining of CD133 in OSE layer (A, B) as well as cortex (C) reveals specific CD133+ cells with relatively higher cell numbers in BL and HG. Area within dotted lines in BN OSE (A) are magnified in (B) while elliptical/spindle shaped CD133+ cells in cortex from various fields were represented in the composite image in (C) of BN and HG. Large CD133+ cells in cortex were also observed. White scale bar?=?50 um; blue scale bar?=?10 um. Secondary antibody employed was conjugated with Alexa fluor 568 and tissue sections were counterstained with nucleus specific dye DAPI. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30121075/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD133-Antibody---BSA-Free-Immunohistochemistry-NB120-16518-img0007.jpg)
![Flow Cytometry: CD133 Antibody - BSA Free [NB120-16518] - Flow Cytometry: CD133 Antibody [DyLight 488] [NB120-16518G] - Flow Cytometry: CD133 Antibody (DyLight488) [NB120-16518G] - Rat bone marrow cells were stained with CD133 (1:100) antibody (20 minutes at 4C), fixed, and then analyzed. Image using the DyLight 488 form of this antibody.](http://images.novusbio.com/fullsize/CD133-Antibody---BSA-Free-Flow-Cytometry-NB120-16518-img0006.jpg)
![Chromatin Immunoprecipitation: CD133 Antibody - BSA Free [NB120-16518] - [NB 120-16518] - Staining CD133 in Bone Marrow Mononuclear Cells by Immunofluorescence.](http://images.novusbio.com/fullsize/CD133-Antibody---BSA-Free-Chromatin-Immunoprecipitation-NB120-16518-img0005.jpg)
![Immunohistochemistry-Paraffin: CD133 Antibody - BSA Free [NB120-16518] - Staining of CD133 in human hepatocarcinoma (HC) tissue. NB120-16518 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.](http://images.novusbio.com/fullsize/CD133-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB120-16518-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD133 Antibody [NBP2-44250] - NIH 3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-mCD133 (NBP2-44250) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/CD133-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-44250-img0002.jpg "Immunocytochemistry/Immunofluorescence: CD133 Antibody [NBP2-44250] - NIH 3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-mCD133 (NBP2-44250) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Western Blot: CD133 Antibody [NBP2-44250] - Total mouse liver protein was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 mg/mL anti-mCD133 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/CD133-Antibody-Western-Blot-NBP2-44250-img0001.jpg)
![Immunohistochemistry-Paraffin: CD133 Antibody (3F10) [NBP2-37741] - Analysis of human esophageal cancer tissues section using mouse anti-CD133 antibody (clone 3F10). The staining was developed using HRP-conjugated secondary antibody and DAB method which followed counterstaining of nuclei with hematoxylin.](http://images.novusbio.com/fullsize/CD133-Antibody-3F10-Immunohistochemistry-Paraffin-NBP2-37741-img0005.jpg "Immunohistochemistry-Paraffin: CD133 Antibody (3F10) [NBP2-37741] - Analysis of human esophageal cancer tissues section using mouse anti-CD133 antibody (clone 3F10). The staining was developed using HRP-conjugated secondary antibody and DAB method which followed counterstaining of nuclei with hematoxylin.")
![Immunohistochemistry-Paraffin: CD133 Antibody (3F10) [NBP2-37741] - Analysis of human breast cancer tissues section using mouse anti-CD133 antibody (clone 3F10). The staining was developed using HRP-conjugated secondary antibody and DAB method which followed counterstaining of nuclei with hematoxylin.](http://images.novusbio.com/fullsize/CD133-Antibody-3F10-Immunohistochemistry-Paraffin-NBP2-37741-img0006.jpg)
![Flow Cytometry: CD133 Antibody (3F10) [NBP2-37741] - Flow cytometric analysis of Hela cells using CD133 mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/CD133-Antibody-3F10-Flow-Cytometry-NBP2-37741-img0002.jpg)
![ELISA: CD133 Antibody (3F10) [NBP2-37741] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CD133-Antibody-3F10-ELISA-NBP2-37741-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD133 Antibody (3F10) [NBP2-37741] - Upregulations of TIPRL, LC3 and CD133 in HCCs. Human tissues were stained with the indicated antibodies followed by confocal observation. The representative images were selected from normal and HCCs groups, respectively. DAPI was used for nucleus staining, and scar bars, 20 (inserted) and 100 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-53191-5) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD133-Antibody-3F10-Immunocytochemistry-Immunofluorescence-NBP2-37741-img0007.jpg)
![Immunocytochemistry/ Immunofluorescence: CD133 Antibody (3F10) - BSA Free [NBP2-37741] - Significant correlations between levels of TIPRL, LC3 & CD133 in liver cancers. (a–c) The statistically significant associations between levels of TIPRL, LC3 & CD133 in each grade of liver cancers were determined by the Spearman correlation. Each dot indicates a single patient. (d) The representative images were selected from each grade of human liver cancer tissues that were stained with indicated antibodies. DAPI was used for nucleus staining, & scar bar, 100 μm. (e) Quantitative analyses of TIPRL, LC3 & CD133 were conducted according to each grade of liver cancers. (n), the number of samples is indicated. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31727942), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-37741_mouse-monoclonal-cd133-antibody-3f10-310202415371917.jpg)
![Western Blot: CD133 Antibody (3Y4A5) [NBP3-15310] - Western blot analysis of extracts of HT-29 cells, using CD133 antibody (NBP3-15310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/CD133-Antibody-3Y4A5-Western-Blot-NBP3-15310-img0003.jpg "Western Blot: CD133 Antibody (3Y4A5) [NBP3-15310] - Western blot analysis of extracts of HT-29 cells, using CD133 antibody (NBP3-15310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: CD133 Antibody (3Y4A5) [NBP3-15310] - Immunohistochemistry of paraffin-embedded human colon carcinoma using CD133 Rabbit mAb (NBP3-15310) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CD133-Antibody-3Y4A5-Immunohistochemistry-Paraffin-NBP3-15310-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CD133 Antibody (3Y4A5) [NBP3-15310] - Western blot analysis of extracts from normal (control) and CD133 knockout (KO) HCT116 cells, using CD133 antibody (NBP3-15310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/CD133-Antibody-3Y4A5-Knockout-Validated-NBP3-15310-img0001.jpg)
![Western Blot: CD133 Antibody (3Y4A5) [CD133] - Western blot analysis of lysates from HT-29 cells, using CD133 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-15310_rabbit-cd133-mab-3y4a5-52202517192943.jpg)
![Immunohistochemistry: CD133 Antibody (3Y4A5) [CD133] - Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using CD133 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15310_rabbit-cd133-mab-3y4a5-522025172608.jpg)
![Immunohistochemistry: CD133 Antibody (3Y4A5) [CD133] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using CD133 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15310_rabbit-cd133-mab-3y4a5-52202517251995.jpg)
![Western Blot: CD133 Antibody [NBP2-44249] - Total mouse placenta protein was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 mg/ml anti-mCD133 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/CD133-Antibody-Western-Blot-NBP2-44249-img0001.jpg "Western Blot: CD133 Antibody [NBP2-44249] - Total mouse placenta protein was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 mg/ml anti-mCD133 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: CD133 Antibody [NBP2-44249] - NIH/3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-mCD133 (M30) (NBP2-44249) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/CD133-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-44249-img0002.jpg)
![Immunohistochemistry-Paraffin: CD133 Antibody [NBP2-59789] - Staining in human cervix, uterine and liver tissues using NBP2-59789 antibody. Corresponding PROM1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD133-Antibody-Immunohistochemistry-Paraffin-NBP2-59789-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD133 Antibody [NBP2-59789] - Staining in human cervix, uterine and liver tissues using NBP2-59789 antibody. Corresponding PROM1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: CD133 Antibody [NBP2-59789] - Analysis in human cell line CACO-2 and human cell line PC-3.](http://images.novusbio.com/fullsize/CD133-Antibody-Western-Blot-NBP2-59789-img0005.jpg)
![Immunohistochemistry-Paraffin: CD133 Antibody [NBP2-59789] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/CD133-Antibody-Immunohistochemistry-Paraffin-NBP2-59789-img0011.jpg)
![Immunohistochemistry-Paraffin: CD133 Antibody [NBP2-59789] - Staining of human breast shows moderate to strong membranous and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CD133-Antibody-Immunohistochemistry-Paraffin-NBP2-59789-img0006.jpg)
![Immunohistochemistry-Paraffin: CD133 Antibody [NBP2-59789] - Staining of human cervix shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/CD133-Antibody-Immunohistochemistry-Paraffin-NBP2-59789-img0007.jpg)
![Immunohistochemistry-Paraffin: CD133 Antibody [NBP2-59789] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/CD133-Antibody-Immunohistochemistry-Paraffin-NBP2-59789-img0008.jpg)
![Immunohistochemistry-Paraffin: CD133 Antibody [NBP2-59787] - Staining in human placenta and skeletal muscle tissues.. Corresponding PROM1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD133-Antibody-Immunohistochemistry-Paraffin-NBP2-59787-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD133 Antibody [NBP2-59787] - Staining in human placenta and skeletal muscle tissues.. Corresponding PROM1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: CD133 Antibody [NBP2-59787] - Analysis in human cell line CACO-2 and human cell line HEK 293.](http://images.novusbio.com/fullsize/CD133-Antibody-Western-Blot-NBP2-59787-img0010.jpg)
![Immunohistochemistry-Paraffin: CD133 Antibody [NBP2-59787] - Staining of human placenta shows strong membranous positivity in decidual cells.](http://images.novusbio.com/fullsize/CD133-Antibody-Immunohistochemistry-Paraffin-NBP2-59787-img0005.jpg)
![Immunohistochemistry-Paraffin: CD133 Antibody [NBP2-59787] - Staining of human endometrium shows strong positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/CD133-Antibody-Immunohistochemistry-Paraffin-NBP2-59787-img0006.jpg)
![Immunohistochemistry-Paraffin: CD133 Antibody [NBP2-59787] - Staining of human skeletal muscle shows moderate to strong positivity in myocytes.](http://images.novusbio.com/fullsize/CD133-Antibody-Immunohistochemistry-Paraffin-NBP2-59787-img0007.jpg)
![Immunohistochemistry-Paraffin: CD133 Antibody [NBP2-59787] - Staining of human kidney shows moderate to strong positivity in apical membrane in cells in tubules.](http://images.novusbio.com/fullsize/CD133-Antibody-Immunohistochemistry-Paraffin-NBP2-59787-img0008.jpg)
![Immunohistochemistry-Paraffin: CD133 Antibody (PROM/6316) [NBP3-07898] - Formalin-fixed, paraffin-embedded human papillary renal cell carcinoma stained with CD133 / Prominin Mouse Monoclonal Antibody (PROM/6316).](http://images.novusbio.com/fullsize/CD133-Antibody-PROM-6316-Immunohistochemistry-Paraffin-NBP3-07898-img0002.jpg "Immunohistochemistry-Paraffin: CD133 Antibody (PROM/6316) [NBP3-07898] - Formalin-fixed, paraffin-embedded human papillary renal cell carcinoma stained with CD133 / Prominin Mouse Monoclonal Antibody (PROM/6316).")
![Immunohistochemistry: CD133 Antibody (PROM/6316) [NBP3-07898] - Formalin-fixed, paraffin-embedded human papillary renal cell carcinoma stained with CD133 / Prominin Mouse Monoclonal Antibody (PROM/6316).](http://images.novusbio.com/fullsize/CD133-Antibody-PROM-6316-Immunohistochemistry-NBP3-07898-img0001.jpg)
![Western Blot: CD133 Antibody (HL1271) - Azide and BSA Free [NBP3-25403] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD133 antibody [HL1271] (NBP3-25403) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25403_rabbit-cd133-mab-hl1271-azide-and-bsa-free-2120249342744.jpg "Western Blot: CD133 Antibody (HL1271) - Azide and BSA Free [NBP3-25403] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD133 antibody [HL1271] (NBP3-25403) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: CD133 Antibody (HL1271) - Azide and BSA Free [NBP3-25403] - CD133 antibody [HL1271] detects CD133 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. CD133 stained by CD133 antibody [HL1271] (NBP3-25403) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25403_rabbit-cd133-mab-hl1271-azide-and-bsa-free-2120249393567.jpg)
![Immunohistochemistry-Paraffin: CD133 Antibody (HL1271) - Azide and BSA Free [NBP3-25403] - CD133 antibody [HL1271] detects CD133 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. CD133 stained by CD133 antibody [HL1271] (NBP3-25403) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25403_rabbit-cd133-mab-hl1271-azide-and-bsa-free-2120249355566.jpg)
![Western Blot: CD133 Antibody (HL1271) - Azide and BSA Free [NBP3-25403] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD133 antibody [HL1271] (NBP3-25403) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25403_rabbit-cd133-mab-hl1271-azide-and-bsa-free-2120249374967.jpg)