CCR7 Antibody

Chemokine receptor 7 (CCR7) is a G-protein coupled receptor (GPCR) that binds chemokine ligand 19 (CCL19) and chemokine ligand 21 (CCL21) and, together, this receptor-ligand interaction functions in inflammatory and adaptive immune response (1,2). The CCR7 protein contains 7-transmembrane spanning alpha helices and is 378 amino acids (aa) in length, with a theoretical molecular weight of 42.8 kDa (3,4). CCR7 is expressed on several cells within the immune system, including naive T cells, central memory T cells, regulatory T cells, naive B cells, a subset of double negative and single positive thymocytes, and mature dendritic cells (DCs) (1, 3).

The primary role of the CCR7/CCL19/CCL21 chemokine signaling axis is homing T cells and DCs to lymph nodes and lymphoid tissues to initiate an immune response (1,2,5,6). In the context of cancer, the CCR7 signaling axis appears to have two opposing roles (2). Downregulation of CCR7 on CD8+ T cells contributes to effector cell migration and anti-cancer activities via cytotoxic tumor-infiltrating lymphocytes (2). However, upregulation of CCR7 by cancer cells can result in cancer cell migration and metastasis (2). Overexpression of CCR7 has been implicated in a variety of cancers including breast, cervical, gastric, head and neck cell carcinoma, and prostate (1,2,7). Studies in breast cancer have found that hypoxia increases CCR7 expression, and this activation can affect cancer cell invasion, extravasation, proliferation, angiogenesis, and metastasis through induction of multiple signaling transduction pathways such as PI3K/AKT, MAPK, and JAK/STAT (5,7).

Given its important role in inflammation and immune response, several strategies have been employed to target the CCR7 signaling axis for cancer immunotherapy (2). Some cancer immunotherapies under investigation include intra-tumoral administration of CCL19 and CCL21, introduction of patient-derived cells transfected to express CCR7 or its ligands, and vaccines (2). Further interrogation of CCR7/CCL19/CCL21 signaling axis is required to develop better therapeutic strategies for cancer treatment.

References:

1. Comerford, I., Harata-Lee, Y., Bunting, M. D., Gregor, C., Kara, E. E., & McColl, S. R. (2013). A myriad of functions and complex regulation of the CCR7/CCL19/CCL21 chemokine axis in the adaptive immune system. Cytokine & growth factor reviews, 24(3), 269-283. https://doi.org/10.1016/j.cytogfr.2013.03.001

2. Salem, A., Alotaibi, M., Mroueh, R., Basheer, H. A., & Afarinkia, K. (2021). CCR7 as a therapeutic target in Cancer. Biochimica et biophysica acta. Reviews on cancer, 1875(1), 188499. https://doi.org/10.1016/j.bbcan.2020.188499

3. Yan, Y., Chen, R., Wang, X., Hu, K., Huang, L., Lu, M., & Hu, Q. (2019). CCL19 and CCR7 Expression, Signaling Pathways, and Adjuvant Functions in Viral Infection and Prevention. Frontiers in cell and developmental biology, 7, 212. https://doi.org/10.3389/fcell.2019.00212

4. Uniprot (P32248)

5. Korbecki, J., Grochans, S., Gutowska, I., Barczak, K., & Baranowska-Bosiacka, I. (2020). CC Chemokines in a Tumor: A Review of Pro-Cancer and Anti-Cancer Properties of Receptors CCR5, CCR6, CCR7, CCR8, CCR9, and CCR10 Ligands. International journal of molecular sciences, 21(20), 7619. https://doi.org/10.3390/ijms21207619

6. Sanchez-Sanchez, N., Riol-Blanco, L., & Rodriguez-Fernandez, J. L. (2006). The multiple personalities of the chemokine receptor CCR7 in dendritic cells. Journal of immunology (Baltimore, Md. : 1950), 176(9), 5153-5159. https://doi.org/10.4049/jimmunol.176.9.5153

7. Rizeq, B., & Malki, M. I. (2020). The Role of CCL21/CCR7 Chemokine Axis in Breast Cancer Progression. Cancers, 12(4), 1036. https://doi.org/10.3390/cancers12041036

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: FLOW, ICC/IF, IF/IHC, IHC-Fr, IHC-P, In Vivo.

Showing Publications 1 - 10 of 43. Show All 43 Publications. Collapse Publications.

Publications using NB100-712	Applications	Species
Zhao T, Chu Z, Chu C et al. Macrophages induce gingival destruction via Piezo1-mediated MMPs-degrading collagens in periodontitis Frontiers in Immunology 2023-05-16 [PMID: 37261355] (ICC/IF, Mouse, Human) Details: Dilution: 1:100	ICC/IF	Mouse, Human
Lian Q, Zheng S, Shi Z et al. Using a degradable three-layer sandwich-type coating to prevent titanium implant infection with the combined efficient bactericidal ability and fast immune remodeling property Acta biomaterialia 2022-10-25 [PMID: 36306986] (IHC-P, Rat)	IHC-P	Rat
Hu XH, Li ZH, Muyayalo KP Et al. A newly intervention strategy in preeclampsia: Targeting PD-1/Tim-3 signaling pathways to modulate the polarization of decidual macrophages FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2022-01-01 [PMID: 34847253]
Guo X, Li M, Qi W Et Al. Serial cellular events in bone formation initiated by calcium phosphate ceramics Acta biomaterialia 2021-07-22 [PMID: 34303865] (IF/IHC)	IF/IHC
Ma Y, Wei C, Qi X et al. Schistosoma japonicum-derived peptide SJMHE1 promotes peripheral nerve repair through a macrophage-dependent mechanism American journal of translational research 2021-03-15 [PMID: 33841657] (IF/IHC, Rat)	IF/IHC	Rat
Kim MK, Kim Y, Park S et al. Effects of Steady Low-Intensity Exercise on High-Fat Diet Stimulated Breast Cancer Progression Via the Alteration of Macrophage Polarization Integr Cancer Ther 2020-09-10 [PMID: 32909498] (IHC-P, Mouse)	IHC-P	Mouse
Li M, Guo X, Qi W et al. Macrophage polarization plays roles in bone formation instructed by calcium phosphate ceramics J. Mater. Chem. B 2020-01-22 [PMID: 32067012]
Jia Y, Yang W, Zhang K et al. Nanofiber arrangement regulates peripheral nerve regeneration through differential modulation of macrophage phenotypes Acta Biomaterialia 2018-10-01 [PMID: 30541701] (In Vivo, Rat)	In Vivo	Rat
Wei F, Liu G, Guo Y et al. Blood prefabricated hydroxyapatite/tricalcium phosphate induces ectopic vascularized bone formation via modulating the osteoimmune environment Biomater Sci 2018-06-22 [PMID: 29931022] (IF/IHC)	IF/IHC
Yuan XL, Zhao YP, Huang J et al. A Kv1.3 channel-specific blocker alleviates neurological impairment through inhibiting T-cell activation in experimental autoimmune encephalomyelitis CNS Neurosci Ther 2018-03-25 [PMID: 29577640] (FLOW, Rat)	FLOW	Rat
Boulding T, McCuaig RD, Tan A et al. LSD1 activation promotes inducible EMT programs and modulates the tumour microenvironment in breast cancer. Sci Rep 2018-01-08 [PMID: 29311580] (ICC/IF, Human)	ICC/IF	Human
Wang RH, Zhou Y, Xiao Y. RANKL-induced M1 macrophages are involved in bone formation. Bone Res. [PMID: 29263936] (IHC-P, Rat)	IHC-P	Rat
Ma T, Luan SL, Huang H et al. Upregulation of CC Chemokine Receptor 7 (CCR7) Enables Migration of Xenogeneic Human Adipose-Derived Mesenchymal Stem Cells to Rat Secondary Lymphoid Organs Med. Sci. Monit 2016-12-30 [PMID: 28035134] (FLOW, Rat)	FLOW	Rat
Reeves AR, Spiller KL, Freytes DO et al. Controlled release of cytokines using silk-biomaterials for macrophage polarization. Biomaterials 2015-09-21 [PMID: 26421484] (ICC/IF, Human)	ICC/IF	Human
Kumar VA, Shi S, Wang BK et al. Drug-triggered and cross-linked self-assembling nanofibrous hydrogels J. Am. Chem. Soc. 2015-04-15 [PMID: 25831137] (IHC-P, Rat)	IHC-P	Rat
Vitale F, Summerson SR, Aazhang B et al. Neural Stimulation and Recording with Bidirectional, Soft Carbon Nanotube Fiber Microelectrodes. ACS Nano. 2015-03-31 [PMID: 25803728] (IHC-Fr, Rat)	IHC-Fr	Rat
Pytte CL, Parent C, Wildstein S, Varghese C, Oberlander S. Deafening decreases neuronal incorporation in the zebra finch caudomedial nidopallium (NCM). Behav Brain Res;211(2):141-7. 2010-08-25 [PMID: 20346987] (IF/IHC)	IF/IHC
Sato M, Hirayama S, Hwang DM et al. The Role of Intrapulmonary De Novo Lymphoid Tissue in Obliterative Bronchiolitis after Lung Transplantation. J Immunol;182(11):7307-7316. 2009-01-01 [PMID: 19454728]
Lassmann J, Sliwoski J, Chang A, Canning DA, Zderic SA. Deletion of One SERCA2 Allele Confers Protection Against Bladder Wall Hypertrophy in a Murine Model of Partial Bladder Outlet Obstruction. Am J Physiol Regul Integr Comp Physiol;R58-65. 2008-01-01 [PMID: 17977917] (IF/IHC)	IF/IHC
Parker MA, Corliss DA, Gray B, Anderson JK, Bobbin RP, Snyder EY, Cotanche DA. Neural stem cells injected into the sound-damaged cochlea migrate throughout the cochlea and express markers of hair cells, supporting cells, and spiral ganglion cells. Hear Res;232(1-2):29-43. 2007-10-01 [PMID: 17659854] (IF/IHC)	IF/IHC
Tokuda M, Kadokawa Y, Kurahashi H, Marunouchi T. Biol Reprod. CDH1 Is a Specific Marker for Undifferentiated Spermatogonia in Mouse Testes. 76(1):130-141. 2007-01-01 [PMID: 17035642] (ICC/IF)	ICC/IF
Wang Y, Tang Q, Maul GG, Yuan Y. Kaposi's sarcoma-associated herpesvirus ori-Lyt-dependent DNA replication: dual role of replication and transcription activator. Virol;80(24):12171-86. 2006-12-01 [PMID: 17020951] (ICC/IF)	ICC/IF
Van Kampen JM, Eckman CB. Dopamine D3 receptor agonist delivery to a model of Parkinson's disease restores the nigrostriatal pathway and improves locomotor behavior. J Neurosci;26(27):7272-80. 2006-07-05 [PMID: 16822985] (IF/IHC)	IF/IHC
Dunlap KD, Castellano JF, Prendaj E. Social interaction and cortisol treatment increase cell addition and radial glia fiber density in the diencephalic periventricular zone of adult electric fish, Apteronotus leptorhynchus. Horm Behav. 50(1):10-7. 2006-06-01 [PMID: 16584732] (IF/IHC)	IF/IHC
Ljungkvist, A S E, Bussink, J, Kaanders, J H A M, Wiedenmann, N E, Vlasman, R van der Kogel, A J. Dynamics of Hypoxia, Proliferation and Apoptosis after Irradiation in a Murine Tumor Model. Radiat Res 165(3): 326- 336. 2006-03-01 [PMID: 16494521] (ICC/IF)	ICC/IF
Lamb CA, Helguero LA, Giulianelli S, Soldati R, Vanzulli SI, Molinolo A, Lanari C. Antisense oligonucleotides targeting the progesterone receptor inhibit hormone-independent breast cancer growth in mice. Breast Cancer Res;7(6):R1111-21. 2005-01-01 [PMID: 16457691] (IF/IHC)	IF/IHC
Zecevic N, Chen Y, Filipovic R. Contributions of cortical subventricular zone to the development of the human cerebral cortex. J Comp Neurol;491(2):109-22. 2005-10-17 [PMID: 16127688] (IF/IHC)	IF/IHC
Fainaru, O et al. Accelerated chemokine receptor 7-mediated dendritic cell migration in Runx3 knockout mice the spontaneous development of asthma-like disease. PNAS. 102(30): 10598-10603. 2005-01-01 [PMID: 16027362]
Walker, SR et al. Murine neuroblastoma attenuates dendritic cell cysteine cysteine receptor 7 (CCR7) expression. J. Pediatr. Surg. 40(6): 983-987. 2005-01-01 [PMID: 15991182]
Gattinoni, L et al. Acquisition of full effector function in vitro paradoxiacally impairs the in vivo antitumor efficacy of adoptively transferred CD8+ T cells. J. Clin. Invest. 115(6): 1616-1626. 2005-01-01 [PMID: 15931392]
Cinalli, RM et al. T cell homrostasis requires G protein-coupled receptor-mediated access to trophic signals that promote growth inhibit chemotaxis. Eur. J. Immunol. 35(3): 786-795. 2005-01-01 [PMID: 15719365]
Ruitenberg MJ, Levison DB, Lee SV, Verhaagen J, Harvey AR, Plant GW. NT-3 expression from engineered olfactory ensheathing glia promotes spinal sparing and regeneration. Brain;128(Pt 4):839-53 (ICC). 2005-04-01 [PMID: 15716305]
Sakai N, Iseki K, Suzuki S, Mori T, Hagino S, Zhang Y, Yokoya S, Kawasaki Y, Suzuki J, Isome M, Wada I, Homma Y, Suzuki H. Uninephrectomy induces progressive glomerulosclerosis and apoptosis in anti-Thy1 glomerulonephritis. Pathol Int;55(1):19-26. 2005-01-01 [PMID: 15660699] (IF/IHC)	IF/IHC
Guo Y, Harwalkar J, Stacey DW, Hitomi M. Destabilization of cyclin D1 message plays a critical role in cell cycle exit upon mitogen withdrawal. Oncogene;24(6):1032-42. 2005-02-03 [PMID: 15592507] (ICC/IF)	ICC/IF
Koretomo, R et al. Augumentation of the migratory ability of DC-based vaccine into regional lymph nodes by efficient CCR7 gene transduction. Gene Ther. 12(2): 129-139. 2005-01-01 [PMID: 15483669]
Cannon JD, Cherian-Shaw M, Chaffin CL. Proliferation of rat granulosa cells during the periovulatory interval. Endocrinology;146(1):414-22. 2005-01-01 [PMID: 15375025] (IF/IHC)	IF/IHC
Wu FY, Chen H, Wang SE, ApRhys CM, Liao G, Fujimuro M, Farrell CJ, Huang J, Hayward SD, Hayward GS. CCAAT/enhancer binding protein alpha interacts with ZTA and mediates ZTA-induced p21(CIP-1) accumulation and G(1) cell cycle arrest during the Epstein-Barr virus lytic cycle. J Virol;77(2):1481-500. 2003-01-01 [PMID: 12502863] (ICC/IF)	ICC/IF
Wang N, Hurley P, Pytte C, Kirn JR. Vocal control neuron incorporation decreases with age in the adult zebra finch. J Neurosci;22(24):10864-70. 2002-12-15 [PMID: 12486180] (IF/IHC)	IF/IHC
Liao G, Wu FY, Hayward SD. Interaction with the Epstein-Barr virus helicase targets Zta to DNA replication compartments. J Virol;75(18):8792-802. 2001-09-01 [PMID: 11507224] (ICC/IF)	ICC/IF
Leferovich JM, Bedelbaeva K, Samulewicz S, Zhang XM, Zwas D, Lankford EB, Heber-Katz E. Heart regeneration in adult MRL mice. Proc Natl Acad Sci U S A;98(17):9830-5. 2001-08-14 [PMID: 11493713]
Kehl LJ, Fairbanks CA, Laughlin TM, Wilcox GL. Neurogenesis in postnatal rat spinal cord: a study in primary culture. Science;276(5312):586-9. 1997-04-25 [PMID: 9110976] (ICC/IF)	ICC/IF
1 Schweickart, VL et al. Cloning of human mouse EBI1, a lymphoid-specific G-protein-coupled receptor encoded on human chromosome 17q12-q21.2. Genomics. 23: 643-50 (1994). Genomics. 23: 643-50. 1994-01-01 [PMID: 7851893]
Campana D, Coustan-Smith E, Janossy G. Double and triple staining methods for studying the proliferative activity of human B and T lymphoid cells. J Immunol Methods;107(1):79-88. 1988-02-24 [PMID: 3257781] (ICC/IF)	ICC/IF
Show All 43 Publications. Collapse Publications.

Array NB100-712

• CCR7 Antibodies
• CCR7 ELISA Kits
• CCR7 Lysate
• CCR7 Proteins