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EVI-1 Antibody

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Western Blot: EVI-1 Antibody [NBP2-48848] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4
Western Blot: EVI-1 Antibody [NBP2-48848] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4
Immunocytochemistry/ Immunofluorescence: EVI-1 Antibody [NBP2-48848] - Staining of human cell line HEK 293 shows localization to nuclear speckles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: EVI-1 Antibody [NBP2-48848] - Staining of human stomach shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: EVI-1 Antibody [NBP2-48848] - Staining of human colon shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: EVI-1 Antibody [NBP2-48848] - Staining of human kidney shows strong nuclear positivity in cells in glomeruli.
Immunohistochemistry-Paraffin: EVI-1 Antibody [NBP2-48848] - Staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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EVI-1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: ATSSPHSELESTGAILDDKEDAYFTEIRNFIGNSNHGSQSPRNVEERMNGSHFKDEKALVTSQNSDLLDDEEVEDEVLLDEEDEDNDITGKTGKEPVTSNLHEGNPEDDYEETSALEMSCKTSPVRYKEEEY
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MECOM
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
EVI-1 Recombinant Protein Antigen (NBP2-48848PEP)
Publications
Read Publication using NBP2-48848.

Reactivity Notes

Mouse (86%), Rat (87%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for EVI-1 Antibody

  • AML1-EVI-1 fusion protein
  • AML1-EVI-1
  • ecotropic viral integration site 1
  • Ecotropic virus integration site 1 protein homolog
  • EVI1
  • EVI-1
  • EVI1MDS1-EVI1
  • MDS1 and EVI1 complex locus protein EVI1
  • MDS1 and EVI1 complex locus protein MDS1
  • MDS1 and EVI1 complex locus
  • MDS1
  • MECOM
  • MGC163392
  • MGC97004
  • Myelodysplasia syndrome 1 protein
  • myelodysplasia syndrome 1
  • Myelodysplasia syndrome-associated protein 1
  • oncogene EVI1
  • PRDM3
  • zinc finger protein Evi1

Background

EVI1 (ecotropic viral integration site 1) was originally identified as a gene located in the integration site of ecotropic retroviruses in the mouse genome that resulted in myeloid tumors. EVI1 is a zinc finger transcription factor that plays an important role in development and leukemogenesis. It is an oncogene that can have effects on cell proliferation, differentiation, and apoptosis. Chromosomal translocations involving EVI1 result in chronic myelogenous leukemia (CML). Alternate names for EVI1 include AML1-EVI-1 fusion protein, PRDM3, and MDS1-EV1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EVI-1 Antibody (NBP2-48848)(1)


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Bioinformatics

Gene Symbol MECOM