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CCM2 Antibody (OTI1E9) - Azide and BSA Free

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Western Blot: CCM2 Antibody (OTI1E9) - Azide and BSA Free [NBP2-72235] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunohistochemistry: CCM2 Antibody (OTI1E9) - Azide and BSA Free [NBP2-72235] - Staining of paraffin-embedded Human lung tissue using anti-CCM2 mouse monoclonal antibody.
Western Blot: CCM2 Antibody (OTI1E9) - Azide and BSA Free [NBP2-72235] - Analysis of extracts (35ug) from 9 different cell lines by using anti-CCM2 monoclonal antibody.
Immunohistochemistry: CCM2 Antibody (OTI1E9) - Azide and BSA Free [NBP2-72235] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CCM2 mouse monoclonal antibody.
Immunohistochemistry: CCM2 Antibody (OTI1E9) - Azide and BSA Free [NBP2-72235] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CCM2 mouse monoclonal antibody.
Immunohistochemistry: CCM2 Antibody (OTI1E9) - Azide and BSA Free [NBP2-72235] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CCM2 mouse monoclonal antibody.
Immunohistochemistry: CCM2 Antibody (OTI1E9) - Azide and BSA Free [NBP2-72235] - Staining of paraffin-embedded Human lymph node tissue using anti-CCM2 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI1E9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

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CCM2 Antibody (OTI1E9) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human CCM2(NP_113631) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CCM2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
Theoretical MW
48.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for CCM2 Antibody (OTI1E9) - Azide and BSA Free

  • C7orf22OSM
  • cerebral cavernous malformation 2
  • Cerebral cavernous malformations 2 protein
  • chromosome 7 open reading frame 22
  • malcavernin
  • MGC4067
  • MGC4607
  • MGC74868

Background

p16 INK4a is a tumor suppressor protein. It is a specific inhibitor of Cdk 4 / Cdk 6, and a tumor suppressor involved in the pathogenesis of a variety of malignancies. Recent analyses of the p16 INK4a gene revealed homozygous deletions, nonsense, missense, or frameshift mutations in several human cancers. Although the frequency of p16 INK4a abnormalities is higher in tumor derived cell lines than in unselected primary tumors, significant subsets of clinical cases with aberrant p16 INK4a gene have been reported among melanomas, gliomas, esophageal, pancreatic, lung, and urinary bladder carcinomas, and some types of leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CCM2