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CCL8/MCP-2 Antibody (CCL8/3686)

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Immunohistochemistry-Paraffin: CCL8/MCP-2 Antibody (CCL8/3686) [NBP3-13771] - Formalin-fixed, paraffin-embedded human adrenal gland stained with CCL8/MCP-2 antibody (CCL8/3686). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Immunohistochemistry-Paraffin: CCL8/MCP-2 Antibody (CCL8/3686) [NBP3-13771] - Formalin-fixed, paraffin-embedded human adrenal gland stained with CCL8/MCP-2 antibody (CCL8/3686). HIER: Tris/EDTA, pH9.0, 45min. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
CCL8/3686
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-14174AF350
Alexa Fluor 405
NBP3-14174AF405
Alexa Fluor 488
NBP3-14174AF488
Alexa Fluor 532
NBP3-14174AF532
Alexa Fluor 594
NBP3-14174AF594
Alexa Fluor 647
NBP3-14174AF647
Alexa Fluor 700
NBP3-14174AF700
Alexa Fluor 750
NBP3-14174AF750
Allophycocyanin
NBP3-14174APC
Biotin
NBP3-14174B
DyLight 350
NBP3-14174UV
DyLight 405
NBP3-14174V
DyLight 488
NBP3-14174G
DyLight 550
NBP3-14174R
DyLight 594
NBP3-14174DL594
DyLight 650
NBP3-14174C
DyLight 680
NBP3-14174FR
DyLight 755
NBP3-14174IR
FITC
NBP3-14174F
HRP
NBP3-14174H
Janelia Fluor 549
NBP3-14174JF549
Janelia Fluor 646
NBP3-14174JF646
PE
NBP3-14174PE
PerCP
NBP3-14174PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP3-14174
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CCL8/MCP-2 Antibody (CCL8/3686) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14174)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.
Immunogen
Human recombinant CCL8/MCP-2 protein fragment (around aa1-99) (exact sequence is proprietary) (Uniprot: P80075)
Localization
Secreted
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CCL8
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Theoretical MW
11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for CCL8/MCP-2 Antibody (CCL8/3686)

  • C-C motif chemokine 8
  • CCL8
  • chemokine (C-C motif) ligand 8
  • HC14
  • MCP2
  • MCP-2
  • member 8 (monocyte chemotactic protein 2)
  • monocyte chemoattractant protein 2
  • SCYA10
  • small-inducible cytokine A8

Background

MCP2 and MCP3 are two recently identified monocyte chemotactic proteins produced by human MG63 osteosarcoma cells. Both MCP2 and MCP3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP1. MCP3 also shares 58% amino acid identity with MCP2. Similarly to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CCL8
Uniprot