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CCL5/RANTES Antibody (21445) [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, B/N, CyTOF-ready, Flow
Clone
21445
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

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CCL5/RANTES Antibody (21445) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
E. coli-derived recombinant human CCL5/RANTES
Specificity
Detects human CCL5/RANTES in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with other chemokines, including recombinant human CCL2, 3, 4,  recombinant mouse (rm) CCL3, and rmCCL4.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CCL5
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunohistochemistry
  • Intracellular Staining by Flow Cytometry
  • Neutralization
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for CCL5/RANTES Antibody (21445) [CoraFluor™ 1]

  • CCL5
  • chemokine (C-C motif) ligand 5
  • D17S136Enormally T-expressed, and presumably secreted
  • EoCP
  • Eosinophil chemotactic cytokine
  • RANTES
  • SISd
  • SIS-delta
  • small inducible cytokine A5 (RANTES)
  • small inducible cytokine subfamily A (Cys-Cys), member 5
  • Small-inducible cytokine A5
  • T cell-specific protein P228
  • T-cell specific protein p288
  • TCP228T-cell-specific protein RANTES

Background

RANTES (Regulated-on-activation-normal-T-cell-expressed-and-secreted) is a member of the "intercrine/chemokine" family of cytokines, has been previously shown to be chemotactic for monocytes and T cells of memory/helper phenotype. RANTES has been shown to cause the release of histamine from basophils and activates eosinophils. It also binds to CCR1, CCR3, CCR4 and CCR5 (1). This CC-chemokine enhances antigen-specific T helper (Th) type-1 responses against HIV-1 (2). CC chemokines are small inducible proteins that are related to interleukin 8. In lung adenocarcinoma, RANTES is a predictor of survival while that of interleukin (IL)-8 is associated with a poor prognosis. In several models, tumorigenesis is abolished by RANTES, while it is facilitated by IL-8. Site-directed mutagenesis indicated that regulation of RANTES promoter activity required two nuclear factor (NF)-kappaB response elements but not its activator protein (AP)-1 binding sites (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CCL5