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CCL3/MIP-1 alpha Antibody [Unconjugated]

 (17 Publications)
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Recombinant Human CCL3/MIP‑1 alpha (Catalog # 270-LD) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant HumanCCL3/MIP‑1 alpha (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL3/MIP‑1 alpha Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF-270-NA). The ND50 is typically 2-8 µg/mL.
Recombinant Human CCL3/MIP‑1 alpha (Catalog # 270-LD) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to ...read more
CCL3/MIP-1a was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human CCL3/MIP-1a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-270-NA) at 10 µg/mL overnight at 4 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, B/N, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF270
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF-270-SP
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CCL3/MIP-1 alpha Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human CCL3/MIP-1 alpha
Ala27-Ala92
Accession # P10147
Specificity
Detects human CCL3/MIP‑1 alpha  in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human (rh) MIP-1 beta , rhMIP-1δ, rhMIP-3 alpha , rhMIP-3 beta , and recombinant mouse (rm) MIP-1 alpha is observed. Neutralizes the biological activity of recombinant human CCL3/MIP‑1 alpha . In the chemotaxis assay this antibody will also partially neutralize the biological activity of rhMIP-1 beta at a 20 fold higher IgG concentration. It will not neutralize the biological activity of rmMIP-1 beta or rmMIP-1 alpha .
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CCL3
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 2-8 ug/mL
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Human CCL3/MIP-1 alpha Antibody (Catalog # AF-270-NA)
ELISA Detection: Human CCL3/MIP-1 alpha Biotinylated Antibody (Catalog # BAF270)
Standard: Recombinant Human CCL3/MIP-1 alpha Isoform LD78a (Catalog # 270-LD)
Publications
Read Publications using
AF-270-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL3/MIP-1 alpha Antibody [Unconjugated]

  • C-C motif chemokine 3
  • MIP1-(a)
  • AI323804
  • CCL3
  • chemokine (C-C motif) ligand 3
  • G0S19-1
  • LD78a
  • LD78alpha
  • MIP1 alpha
  • MIP-1 alpha
  • MIP1A
  • MIP-1alpha
  • MIP1-alpha
  • PAT 464.1
  • SCYA3
  • SIS-beta

Background

The macrophage inflammatory proteins -1 alpha and -1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP-1 alpha refers to the products of several independently cloned cDNAs, including LD78, pL78, pAT464, and GOS19. These cDNAs all code for the same human protein that is a homologue of the murine MIP-1 alpha . Mature MIP-1 alpha and MIP-1 beta in both human and mouse share approximately 70% homology at the amino acid level. The MIP‑1 proteins are members of the beta (C-C) subfamily of chemokines.

Both MIP-1 alpha and MIP-1 beta are monocyte chemoattractants in vitro. Additionally, the MIP-1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with MIP-1 alpha and MIP-1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. MIP-1 alpha has also been shown to attract B cells as well as eosinophils. MIP-1 proteins have been reported to have multiple effects on hematopoietic precursor cells and MIP-1 alpha has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. The functional receptor for MIP-1 alpha has been identified as CCR1 and CCR5.

  1. Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CCL3/MIP-1 alpha
Antibody [Unconjugate ...
AF-270-NA
CCL3/MIP-1 alpha Antibody [Unconjugated]
Species: Hu
Applications: WB, IHC, B/N, ELISA(Cap)

Publications for CCL3/MIP-1 alpha Antibody (AF-270-NA)(17)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 6 applications: Antibody Array Development, ELISA Development, Flow Cytometry, Luminex Development, Neutralization, Western Blot.


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Antibody Array Development
(1)
ELISA Development
(3)
Flow Cytometry
(1)
Luminex Development
(4)
Neutralization
(4)
Western Blot
(1)
All Applications
Filter By Species
Human
(14)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using AF-270-NA Applications Species
Kuczkowska, K;Bjerkan, L;Stubsrud, E;Husbyn, HC;Chellappa, S;Hauge, A;Skarshaug, R;Torgersen, ML;Heim, JB;Jørgensen, MJ;Wold, CW;Schleimann, MH;Tolstrup, M;Granum, S;Fredriksen, AB;Pedersen, MW;Norheim, G; A novel SARS-CoV-2 Beta RBD DNA vaccine directly targeted to antigen-presenting cells induces strong humoral and T cell responses Scientific reports 2023-11-02 [PMID: 37919366] (Western Blot, Human) Western Blot Human
Mathieu Claireaux, Rémy Robinot, Jérôme Kervevan, Mandar Patgaonkar, Isabelle Staropoli, Anne Brelot et al. Low CCR5 expression protects HIV-specific CD4+ T cells of elite controllers from viral entry Nature Communications 1/26/2022 [PMID: 35082297]
Nikki P. L. Toledo, Hongzhao Li, Robert W. Omange, Tamara G. Dacoba, Jose Crecente-Campo, Dane Schalk et al. Cervico-Vaginal Inflammatory Cytokine and Chemokine Responses to Two Different SIV Immunogens Frontiers in Immunology 8/25/2020 [PMID: 32983121]
T Kodama, YI Koma, N Arai, A Kido, N Urakawa, M Nishio, M Shigeoka, H Yokozaki CCL3-CCR5 axis contributes to progression of esophageal squamous cell carcinoma by promoting cell migration and invasion via Akt and ERK pathways Lab. Invest., 2020-05-26;0(0):. 2020-05-26 [PMID: 32457351] (Neutralization, Human) Neutralization Human
T Leng, HD Akther, CP Hackstein, K Powell, T King, M Friedrich, Z Christofor, S McCuaig, M Neyazi, CV Arancibia-, J Hagel, F Powrie, RS Peres, V Millar, D Ebner, R Lamichhane, J Ussher, TSC Hinks, E Marchi, C Willberg, P Klenerman TCR and Inflammatory Signals Tune Human MAIT Cells to Exert Specific Tissue Repair and Effector Functions Cell Rep, 2019-09-17;28(12):3077-3091.e5. 2019-09-17 [PMID: 31533032] (Flow Cytometry, Human) Flow Cytometry Human
Walker W, Kurscheid S, Joshi S, Lopez C, Goh G, Choi M, Barakat L, Francis J, Fisher A, Kozal M, Zapata H, Shaw A, Lifton R, Sutton R, Fikrig E Increased Levels of Macrophage Inflammatory Proteins Result in Resistance to R5-Tropic HIV-1 in a Subset of Elite Controllers. J Virol, 2015-03-04;89(10):5502-14. 2015-03-04 [PMID: 25740989] (Neutralization, Human) Neutralization Human
Baschuk N, Wang N, Watt SV et al. NK cell intrinsic regulation of MIP-1? by granzyme M. Cell Death Dis 3/13/2014 [PMID: 24625974]
Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin. J. Immunol., 2009-10-07;183(9):5799-806. 2009-10-07 [PMID: 19812208] (ELISA Development, Human) ELISA Development Human
Hu H, Kwun J, Aizenstein BD, Knechtle SJ Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine. Transplantation, 2009-06-27;87(12):1814-20. 2009-06-27 [PMID: 19543058] (Antibody Array Development, Human) Antibody Array Development Human
Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo. AIDS Res. Hum. Retroviruses, 2008-03-01;24(3):453-62. 2008-03-01 [PMID: 18327985] (Luminex Development, Human) Luminex Development Human
Show All 17 Publications.

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Bioinformatics

Gene Symbol CCL3
Uniprot