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CCL3/MIP-1 alpha Antibody (39624) [Phycoerythrin]

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LPS-stimulated RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse CCL3/MIP-1a PE-conjugated Monoclonal Antibody (Catalog # IC450P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Intracellular Molecules.
LPS-stimulated RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse CCL3/MIP-1a PE-conjugated Monoclonal Antibody (Catalog # IC450P, filled histogram) or isotype control antibody (Catalog # ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
39624
Clonality
Monoclonal
Host
Rat
Conjugate
Phycoerythrin

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Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB450U
Alexa Fluor 405
FAB450V
Alexa Fluor 488
IC450G
Alexa Fluor 532
FAB450X
Alexa Fluor 594
FAB450T
Alexa Fluor 647
FAB450R
Alexa Fluor 700
FAB450N
Alexa Fluor 750
FAB450S
Allophycocyanin
IC450A
Biotin
FAB450B
CoraFluor 1
FAB450CL1
DyLight 350
FAB450D
DyLight 405
FAB450E
DyLight 488
FAB450K
DyLight 550
FAB450L
DyLight 594
FAB450M
DyLight 650
FAB450W
DyLight 680
FAB450Y
DyLight 755
FAB450Z
FITC
FAB450F
HRP
FAB450H
Janelia Fluor 525
FAB450JF525
Janelia Fluor 549
FAB450I
Janelia Fluor 585
FAB450JF585
Janelia Fluor 635
FAB450JF635
Janelia Fluor 646
FAB450J
Janelia Fluor 669
FAB450JF669
PerCP
FAB450C
Unconjugated
MAB450-100
mFluor Violet 450 SE
FAB450MFV450
mFluor Violet 500 SE
FAB450MFV500
mFluor Violet 610 SE
FAB450MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB450-100
Carrier Free
IC450A
Carrier Free
IC450G
Carrier Free
MAB450-SP
Carrier Free
MAB450-500
Carrier Free
MAB450-100
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CCL3/MIP-1 alpha Antibody (39624) [Phycoerythrin] Summary

Immunogen
E. coli-derived recombinant mouse CCL3/MIP-1 alpha
Ala24-Ala92
Accession # P10855
Specificity
Detects mouse CCL3/MIP‑1 alpha in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse CCL4/MIP‑1 beta  is observed and no cross-reactivity with recombinant human CCL1, 2, 3, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 21, 22, 23, 24, 25, 28, recombinant mouse CCL1, 2, 6, 7, 9/10/MIP-1 gamma , 11, 12, 19, 21, 22, 25, 28, recombinant rat CCL2 or CCL20 is observed. Neutralizes the biological activity of recombinant mouse CCL3 and will not block the biological activity of recombinant human CCL3.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
CCL3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells
Publications
Read Publications using
IC450P in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL3/MIP-1 alpha Antibody (39624) [Phycoerythrin]

  • C-C motif chemokine 3
  • MIP1-(a)
  • AI323804
  • CCL3
  • chemokine (C-C motif) ligand 3
  • G0S19-1
  • LD78a
  • LD78alpha
  • MIP1 alpha
  • MIP-1 alpha
  • MIP1A
  • MIP-1alpha
  • MIP1-alpha
  • PAT 464.1
  • SCYA3
  • SIS-beta

Background

The macrophage inflammatory proteins 1 alpha and 1 beta , two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse CCL3/MIP-1 alpha shares approximately 77% and 70% amino acid identity with human CCL3/MIP-1 alpha and mouse CCL4/MIP-1 beta , respectively. MIP‑1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP‑1 proteins are members of the beta  (C‑C) subfamily of chemokines.

Both CCL3 and CCL4 are monocyte chemoattractants in vitro. Additionally, the MIP‑1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with CCL3 and CCL4 preferentially attracting CD8+ and CD4+ T cells, respectively. CCL3 has also been shown to attract B cells as well as eosinophils. MIP‑1 proteins have been reported to have multiple effects on hematopoietic precursor cells, and CCL3 has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. In the same assays, CCL4 was reported to be much less active. The functional receptor for CCL3 has been identified as CCR1 and CCR5.

  1. Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CCL3/MIP-1 alpha
Antibody (39624) [Phy ...
IC450P
CCL3/MIP-1 alpha Antibody (39624) [Phycoerythrin]
Species: Mu
Applications: Flow

Publications for CCL3/MIP-1 alpha Antibody (IC450P)(8)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Flow Cytometry.


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Flow Cytometry
(7)
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Mouse
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Showing Publications 1 - 8 of 8.
Publications using IC450P Applications Species
MS Abdel-Hake, S Manne, JC Beltra, E Stelekati, Z Chen, K Nzingha, MA Ali, JL Johnson, JR Giles, D Mathew, AR Greenplate, G Vahedi, EJ Wherry Epigenetic scarring of exhausted T cells hinders memory differentiation upon eliminating chronic antigenic stimulation Nature Immunology, 2021-07-26;22(8):1008-1019. 2021-07-26 [PMID: 34312545] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Zeyu Chen, Eri Arai, Omar Khan, Zhen Zhang, Shin Foong Ngiow, Yuan He et al. In vivo CD8+ T cell CRISPR screening reveals control by Fli1 in infection and cancer. Cell 3/1/2021 [PMID: 33636129]
EC Smith, KJ Limbach, N Rangel, K Oda, JS Bolton, M Du, K Gowda, J Wang, JK Moch, S Sonawane, R Velasco, A Belmonte, R Danner, JM Lumsden, NB Patterson, M Sedegah, MR Hollingdal, TL Richie, JB Sacci, ED Villasante, JC Aguiar Novel malaria antigen Plasmodium yoelii E140 induces antibody-mediated sterile protection in mice against malaria challenge PLoS ONE, 2020-05-14;15(5):e0232234. 2020-05-14 [PMID: 32407410] (Flow Cytometry, Mouse) Flow Cytometry Mouse
E Stelekati, Z Chen, S Manne, M Kurachi, MA Ali, K Lewy, Z Cai, K Nzingha, LM McLane, JL Hope, AJ Fike, PD Katsikis, EJ Wherry Long-Term Persistence of Exhausted CD8�T Cells in Chronic Infection Is Regulated by MicroRNA-155 Cell Rep, 2018-05-15;23(7):2142-2156. 2018-05-15 [PMID: 29768211] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Z Chen, E Stelekati, M Kurachi, S Yu, Z Cai, S Manne, O Khan, X Yang, EJ Wherry miR-150 Regulates Memory CD8�T Cell Differentiation via c-Myb Cell Rep, 2017-09-12;20(11):2584-2597. 2017-09-12 [PMID: 28903040] (Flow Cytometry, Mouse) Flow Cytometry Mouse
B Xiang, TR Baybutt, L Berman-Boo, MS Magee, SA Waldman, VY Alexeev, AE Snook Prime-Boost Immunization Eliminates Metastatic Colorectal Cancer by Producing High-Avidity Effector CD8(+) T Cells J. Immunol, 2017-03-24;0(0):. 2017-03-24 [PMID: 28341670] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Adam E Snook GUCY2C-directed CAR-T cells oppose colorectal cancer metastases without autoimmunity Oncoimmunology, 2016-09-02;5(10):e1227897. 2016-09-02 [PMID: 27853651] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D Comprehensive assessment of chemokine expression profiles by flow cytometry. J. Clin. Invest., 2010-02-08;120(3):907-23. 2010-02-08 [PMID: 20197626] (Flow Cytometry, Mouse) Flow Cytometry Mouse

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Bioinformatics

Gene Symbol CCL3
Uniprot