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CCL3/MIP-1 alpha Antibody (39624) [Allophycocyanin]

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RAW 264.7 mouse monocyte/macrophage cell line treated with LPS was stained with Rat Anti-Mouse CCL3/MIP-1a APC-conjugated Monoclonal Antibody (Catalog # IC450A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
RAW 264.7 mouse monocyte/macrophage cell line treated with LPS was stained with Rat Anti-Mouse CCL3/MIP-1a APC-conjugated Monoclonal Antibody (Catalog # IC450A, filled histogram) or isotype control antibody (Catalog # ...read more
Maternally derived H-2Dd also impedes fetal growth and vascular remodelling.(a) Density plots showing the proportion of IFN-gamma + or MIP-1 alpha + uNK among cells positive or negative for Ly49A. (b–e) Fold-change in ...read more
Paternal MHC does not educate uNK cells.(a) Density plots showing the proportion of IFN-gamma producing uNK (gd9.5+10.5) in response to NK1.1 cross-linking among cells positive or negative for the indicated Ly49 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
39624
Clonality
Monoclonal
Host
Rat
Conjugate
Allophycocyanin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB450U
Alexa Fluor 405
FAB450V
Alexa Fluor 488
IC450G
Alexa Fluor 532
FAB450X
Alexa Fluor 594
FAB450T
Alexa Fluor 647
FAB450R
Alexa Fluor 700
FAB450N
Alexa Fluor 750
FAB450S
Biotin
FAB450B
CoraFluor 1
FAB450CL1
DyLight 350
FAB450D
DyLight 405
FAB450E
DyLight 488
FAB450K
DyLight 550
FAB450L
DyLight 594
FAB450M
DyLight 650
FAB450W
DyLight 680
FAB450Y
DyLight 755
FAB450Z
FITC
FAB450F
HRP
FAB450H
Janelia Fluor 525
FAB450JF525
Janelia Fluor 549
FAB450I
Janelia Fluor 646
FAB450J
Janelia Fluor 669
FAB450JF669
PerCP
FAB450C
Phycoerythrin
IC450P
Unconjugated
MAB450-100
mFluor Violet 450 SE
FAB450MFV450
mFluor Violet 500 SE
FAB450MFV500
mFluor Violet 610 SE
FAB450MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB450-100
Carrier Free
IC450G
Carrier Free
IC450P
Carrier Free
MAB450-SP
Carrier Free
MAB450-500
Carrier Free
MAB450-100
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CCL3/MIP-1 alpha Antibody (39624) [Allophycocyanin] Summary

Immunogen
E. coli-derived recombinant mouse CCL3/MIP-1 alpha
Ala24-Ala92
Accession # P10855
Specificity
Detects mouse CCL3/MIP‑1 alpha in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse CCL4/MIP‑1 beta  is observed and no cross-reactivity with recombinant human CCL1, 2, 3, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 21, 22, 23, 24, 25, 28, recombinant mouse CCL1, 2, 6, 7, 9/10/MIP-1 gamma , 11, 12, 19, 21, 22, 25, 28, recombinant rat CCL2 or CCL20 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
CCL3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells
Publications
Read Publications using
IC450A in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL3/MIP-1 alpha Antibody (39624) [Allophycocyanin]

  • C-C motif chemokine 3
  • MIP1-(a)
  • AI323804
  • CCL3
  • chemokine (C-C motif) ligand 3
  • G0S19-1
  • LD78a
  • LD78alpha
  • MIP1 alpha
  • MIP-1 alpha
  • MIP1A
  • MIP-1alpha
  • MIP1-alpha
  • PAT 464.1
  • SCYA3
  • SIS-beta

Background

The macrophage inflammatory proteins 1 alpha and 1 beta , two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse CCL3/MIP-1 alpha shares approximately 77% and 70% amino acid identity with human CCL3/MIP-1 alpha and mouse CCL4/MIP-1 beta , respectively. MIP‑1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP‑1 proteins are members of the beta  (C‑C) subfamily of chemokines.

Both CCL3 and CCL4 are monocyte chemoattractants in vitro. Additionally, the MIP‑1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with CCL3 and CCL4 preferentially attracting CD8+ and CD4+ T cells, respectively. CCL3 has also been shown to attract B cells as well as eosinophils. MIP‑1 proteins have been reported to have multiple effects on hematopoietic precursor cells, and CCL3 has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. In the same assays, CCL4 was reported to be much less active. The functional receptor for CCL3 has been identified as CCR1 and CCR5.

  1. Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CCL3/MIP-1 alpha
Antibody (39624) [All ...
IC450A
CCL3/MIP-1 alpha Antibody (39624) [Allophycocyanin]
Species: Mu
Applications: Flow

Publications for CCL3/MIP-1 alpha Antibody (IC450A)(3)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Flow Cytometry.


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Bioinformatics

Gene Symbol CCL3
Uniprot