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CCL3/MIP-1 alpha Antibody (175024)

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Recombinant Cotton Rat CCL3/MIP‑1 alpha (Catalog # 1061-RM) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Cotton Rat CCL3/MIP‑1 alpha (10 ng/mL) is neutralized (green line) by increasing concentrations of Cotton Rat CCL3/MIP‑1 alpha Monoclonal Antibody (Catalog # MAB1061). The ND50 is typically 0.05-0.3 µg/mL.
Recombinant Cotton Rat CCL3/MIP‑1 alpha (Catalog # 1061-RM) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated ...read more

Product Details

Summary
Reactivity CrSpecies Glossary
Applications WB, B/N
Clone
175024
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB1061-SP
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CCL3/MIP-1 alpha Antibody (175024) Summary

Immunogen
E. coli-derived recombinant cotton rat CCL3/MIP-1 alpha
Ala24-Ala92
Accession # AAL26704
Specificity
Detects cotton rat CCL3/MIP-1 alpha in direct ELISAs. In direct ELISAs, this antibody shows approximately 50% cross-reactivity with recombinant human CCL3/MIP‑1 alpha and recombinant cotton rat CCL4/MIP-1 beta and no cross-reactivity with recombinant cotton rat CCL5, recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 8, 9/10/MIP-1 gamma , 11, 12, 17, 19, 20, 21, 22, 24, 25, 27, 28, or recombinant rat CCL20.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CCL3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Neutralization 0.05-0.3 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publication using
MAB1061 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL3/MIP-1 alpha Antibody (175024)

  • C-C motif chemokine 3
  • MIP1-(a)
  • AI323804
  • CCL3
  • chemokine (C-C motif) ligand 3
  • G0S19-1
  • LD78a
  • LD78alpha
  • MIP1 alpha
  • MIP-1 alpha
  • MIP1A
  • MIP-1alpha
  • MIP1-alpha
  • PAT 464.1
  • SCYA3
  • SIS-beta

Background

MIP-1 alpha is a beta family (CC) chemokine and has been designated CCL3. MIP-1 alpha and CCL4/MIP-1 beta , two closely related but distinct proteins, were originally purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Cotton rat CCL3 cDNA encodes a 92 amino acid (aa) precursor protein with a 23 aa putative signal peptide. Mature cotton rat CCL3 shares approximately 70% amino acid identity with human CCL3. CCL3 is expressed in a wide variety of cells, including lymphocytes, fibroblasts, epithelial cells, and monocytes/macrophages. CCL3 has been shown to play an important role in the recruitment of mononuclear cells. Additionally, CCL3 has been reported to have chemoattractant and adhesive effects on lymphocytes, preferentially promoting the chemotaxis of Th1 cells. CCL3 has also been shown to attract B cells, eosinophils, and dendritic cells. In addition, CCL3 augments cytolytic activity of NK cells (1). CCL3 has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. It has been demonstrated that CCL3 can bind the chemokine receptors CCR1 and CCR5 (2).

  1. Robertson, M. (2002) J. Leukoc. Biol. 71:173.
  2. Zlotnik, A. et al. (2000) Immunity 12:121.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CCL3/MIP-1 alpha
Antibody (175024)
MAB1061
CCL3/MIP-1 alpha Antibody (175024)
Species: Cr
Applications: WB, B/N

Publications for CCL3/MIP-1 alpha Antibody (MAB1061)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Neutralization.


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Bioinformatics

Gene Symbol CCL3
Uniprot