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CCL20/MIP-3 alpha Antibody [Unconjugated]

 (7 Publications)
 (1 Review)
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Recombinant Mouse CCL20/MIP‑3 alpha (Catalog #760-M3) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL20/MIP‑3 alpha (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL20/MIP‑3 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF760). The ND50 is typically 0.5-2.0 µg/mL.
Recombinant Mouse CCL20/MIP‑3 alpha (Catalog #760-M3) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to ...read more
Fatty acid induces Ccl20 expression from dermal blood endothelial cells (BECs) and epidermal keratinocytes. (a) Fold induction of Ccl2, Cxcl16, Ccl20 mRNA in the dermis of ND- or HFD-fed mice in the steady state, as ...read more
High-fat diet-induced obesity exacerbates imiquimod-induced psoriatic dermatitis. (a) Comparison of body weight between normal diet (ND)- and high fat diet (HFD)-fed mice 10 weeks after feeding. (b–d) Imiquimod ...read more
High-fat diet-induced obesity exacerbates imiquimod-induced psoriatic dermatitis. (a) Comparison of body weight between normal diet (ND)- and high fat diet (HFD)-fed mice 10 weeks after feeding. (b–d) Imiquimod ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF760
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF760-SP
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CCL20/MIP-3 alpha Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse CCL20/MIP-3 alpha
Ala27-Met96
Accession # Q642U4
Specificity
Detects mouse CCL20/MIP-3 alpha in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CCL20
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Neutralization 0.5-2.0 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF760 in the following applications:
Publications
Read Publications using
AF760 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL20/MIP-3 alpha Antibody [Unconjugated]

  • beta chemokine exodus-1
  • Beta-chemokine exodus-1
  • CC chemokine LARC
  • C-C motif chemokine 20
  • CCL20
  • chemokine (C-C motif) ligand 20
  • CKb4
  • exodus-1
  • LARC
  • LARCLiver and activation-regulated chemokine
  • MIP3 alpha
  • MIP-3 alpha
  • MIP-3a
  • MIP-3-alpha
  • MIP3AMacrophage inflammatory protein 3 alpha
  • SCYA20Small-inducible cytokine A20
  • small inducible cytokine subfamily A (Cys-Cys), member 20
  • ST38

Background

MIP-3 alpha , also known as LARC (Liver and Activation-regulated Chemokine) and Exodus, is one of many novel beta chemokines identified through bioinformatics. Mouse MIP-3 alpha cDNA encodes a 97 amino acid residue precursor protein with a 27 aa residue putative signal peptide that is predicted to be cleaved to form the 70 aa residue mature secreted protein. MIP-3 alpha is distantly related to other beta chemokines (20-28% aa sequence identity). Mouse MIP-3 alpha shares approximately 71% and 63% amino acid sequence homology with rat and human MIP-3 alpha , respectively.

MIP-3 alpha has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung, and epithelial cells of intestinal tissues. The expression of MIP-3 alpha is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant MIP-3 alpha has been shown to be chemotactic for lymphocytes and dendritic cells, and inhibits proliferation of myeloid progenitors in colony formation assays. MIP-3 alpha has now been shown to be a unique functional ligand for CCR6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CCL20/MIP-3 alpha
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AF760
CCL20/MIP-3 alpha Antibody [Unconjugated]
Species: Mu
Applications: WB, B/N

Publications for CCL20/MIP-3 alpha Antibody (AF760)(7)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: Flow Cytometry, IHC-Fr, IHC-P, Neutralization.


Filter By Application
Flow Cytometry
(1)
IHC-Fr
(2)
IHC-P
(1)
Neutralization
(2)
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Mouse
(6)
All Species
Showing Publications 1 - 7 of 7.
Publications using AF760 Applications Species
An L, Kim D, Donahue L et al. Sexual dimorphism in melanocyte stem cell behavior reveals combinational therapeutic strategies for cutaneous repigmentation bioRxiv 5/23/2023 [PMID: 37293072]
M Chen, RR Reed, AP Lane Chronic Inflammation Directs an Olfactory Stem Cell Functional Switch from Neuroregeneration to Immune Defense Cell Stem Cell, 2019-09-12;25(4):501-513.e5. 2019-09-12 [PMID: 31523027] (Neutralization, Mouse) Neutralization Mouse
T Kanaya, S Sakakibara, T Jinnohara, M Hachisuka, N Tachibana, S Hidano, T Kobayashi, S Kimura, T Iwanaga, T Nakagawa, T Katsuno, N Kato, T Akiyama, T Sato, IR Williams, H Ohno Development of intestinal M cells and follicle-associated epithelium is regulated by TRAF6-mediated NF-?B signaling J. Exp. Med., 2018-01-16;0(0):. 2018-01-16 [PMID: 29339448] (IHC-P, Mouse) IHC-P Mouse
Allais L, Kumar S, Debusschere K, Verschuere S, Maes T, De Smet R, Conickx G, De Vos M, Laukens D, Joos G, Brusselle G, Elewaut D, Cuvelier C, Bracke K The Effect of Cigarette Smoke Exposure on the Development of Inflammation in Lungs, Gut and Joints of TNFDeltaARE Mice. PLoS ONE, 2015-11-02;10(11):e0141570. 2015-11-02 [PMID: 26523550] (IHC-Fr, Mouse) IHC-Fr Mouse
Mabuchi T, Singh T, Takekoshi T, Jia G, Wu X, Kao M, Weiss I, Farber J, Hwang S CCR6 is required for epidermal trafficking of gammadelta-T cells in an IL-23-induced model of psoriasiform dermatitis. J Invest Dermatol, 2012-08-16;133(1):164-71. 2012-08-16 [PMID: 22895364] (IHC-Fr, Mouse) IHC-Fr Mouse
Li Z, Burns AR, Miller SB, Smith CW CCL20, gammadelta T cells, and IL-22 in corneal epithelial healing. FASEB J., 2011-04-25;25(8):2659-68. 2011-04-25 [PMID: 21518851] (Neutralization, Mouse) Neutralization Mouse
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D Comprehensive assessment of chemokine expression profiles by flow cytometry. J. Clin. Invest., 2010-02-08;120(3):907-23. 2010-02-08 [PMID: 20197626] (Flow Cytometry, Mouse) Flow Cytometry Mouse

Review for CCL20/MIP-3 alpha Antibody (AF760) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF760:
Filter by Applications
IHC-P
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
 IHC-P Mouse 01/09/2015
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 23203627
SpeciesMouse

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 Verified Customer
01/09/2015
Application: IHC-P
Species: Mouse
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Bioinformatics

Gene Symbol CCL20
Uniprot