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CCL20/MIP-3 alpha Antibody [Biotin]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA(Det)
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Unconjugated
AF760
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF760
Carrier Free
AF760
Carrier Free
AF760-SP
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CCL20/MIP-3 alpha Antibody [Biotin] Summary

Immunogen
E. coli-derived recombinant mouse CCL20/MIP-3 alpha (R&D Systems, Catalog # 760-M3)
Ala27-Met96
Accession # Q642U4
Specificity
Detects mouse MIP-3 alpha  in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) MIP-3 alpha , rhMIP-3 beta , recombinant mouse (rm) MIP-3 beta , recombinant rat MIP-3 alpha , rmMIP-1 gamma , rmMIP-1 alpha , rmMIP-1 beta , and rmMIP-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CCL20
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Mouse CCL20/MIP-3 alpha Antibody (Catalog # MAB7601)
ELISA Detection: Mouse CCL20/MIP-3 alpha Biotinylated Antibody (Catalog # BAF760)
Standard: Recombinant Mouse CCL20/MIP-3 alpha (Catalog # 760-M3)
Readout System
Publications
Read Publications using
BAF760 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL20/MIP-3 alpha Antibody [Biotin]

  • beta chemokine exodus-1
  • Beta-chemokine exodus-1
  • CC chemokine LARC
  • C-C motif chemokine 20
  • CCL20
  • chemokine (C-C motif) ligand 20
  • CKb4
  • exodus-1
  • LARC
  • LARCLiver and activation-regulated chemokine
  • MIP3 alpha
  • MIP-3 alpha
  • MIP-3a
  • MIP-3-alpha
  • MIP3AMacrophage inflammatory protein 3 alpha
  • SCYA20Small-inducible cytokine A20
  • small inducible cytokine subfamily A (Cys-Cys), member 20
  • ST38

Background

MIP-3 alpha , also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. Mouse MIP-3 alpha cDNA encodes a 97 amino acid residue precursor protein with a 27 aa residue putative signal peptide that is predicted to be cleaved to form the 70 aa residue mature secreted protein. MIP-3 alpha is distantly related to other beta chemokines (20 - 28% aa sequence identity). Mouse MIP-3 alpha shares approximately 71 and 63% amino acid sequence homology with rat and human MIP-3 alpha , respectively.

MIP-3 alpha has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung, and epithelial cells of intestinal tissues. The expression of MIP-3 alpha is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant MIP-3 alpha has been shown to be chemotactic for lymphocytes and dendritic cells, and inhibits proliferation of myeloid progenitors in colony formation assays. MIP-3 alpha has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors.

  1. Baba, M. et al. (1997) J. Biol. Chem. 272:14893.
  2. Hromas, R. et al. (1997) Blood 89:3315.
  3. Greaves, D. R. et al. (1997) J. Exp. Med. 186: 857.
  4. Tanaka, Y. et al. (1999) Eur. J. Immunol. 29:633.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CCL20/MIP-3 alpha
Antibody [Biotin]
BAF760
CCL20/MIP-3 alpha Antibody [Biotin]
Species: Mu
Applications: WB, ELISA(Det)

Publications for CCL20/MIP-3 alpha Antibody (BAF760)(5)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: ELISA Development, ELISA Development (Detection), IHC.


Filter By Application
ELISA Development
(1)
ELISA Development (Detection)
(3)
IHC
(1)
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Mouse
(5)
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Showing Publications 1 - 5 of 5.
Publications using BAF760 Applications Species
A Venkatesh, H Nandigam, M Muccioli, M Singh, T Loftus, D Lewis, M Pate, F Benencia Regulation of inflammatory factors by double-stranded RNA receptors in breast cancer cells Immunobiology, 2017-11-22;0(0):. 2017-11-22 [PMID: 29331323] (ELISA Development (Detection), Mouse) ELISA Development (Detection) Mouse
R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3 Cell. Physiol. Biochem., 2017-08-09;42(5):1961-1972. 2017-08-09 [PMID: 28793286] (ELISA Development (Detection), Mouse) ELISA Development (Detection) Mouse
Boyle S, Faulkner J, McColl S, Kochetkova M The chemokine receptor CCR6 facilitates the onset of mammary neoplasia in the MMTV-PyMT mouse model via recruitment of tumor-promoting macrophages. Mol Cancer, 2015-06-06;14(0):115. 2015-06-06 [PMID: 26047945] (ELISA Development (Detection), Mouse) ELISA Development (Detection) Mouse
Baptista A, Olivier B, Goverse G, Greuter M, Knippenberg M, Kusser K, Domingues R, Veiga-Fernandes H, Luster A, Lugering A, Randall T, Cupedo T, Mebius R Colonic patch and colonic SILT development are independent and differentially regulated events. Mucosal Immunol, 2012-09-19;6(3):511-21. 2012-09-19 [PMID: 22990625] (IHC, Mouse) IHC Mouse
Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP Tumor-specific Th17-polarized cells eradicate large established melanoma. Blood, 2008-03-19;112(2):362-73. 2008-03-19 [PMID: 18354038] (ELISA Development, Mouse) ELISA Development Mouse

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Bioinformatics

Gene Symbol CCL20
Uniprot