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CCL20/MIP-3 alpha Antibody [Unconjugated]

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CCL20/MIP-3a was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stumulated with LPS using Human CCL20/MIP-3a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF360) at 10 µg/mL for ...read more
CCL20/MIP‑3a was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human CCL20/MIP‑3a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF360) at 15 µg/mL ...read more
Recombinant Human CCL20/MIP‑3 alpha (Catalog # 360-MP) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that ...read more
CCL20/MIP‑3 alpha was detected in immersion fixed paraffin-embedded sections of Human Testis using Goat Anti-Human CCL20/MIP‑3 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF360) at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, B/N, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price
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CCL20/MIP-3 alpha Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human CCL20/MIP-3 alpha
Ala27-Met96 (Asn95Asp)
Accession # P78556
Specificity
Detects human CCL20/MIP-3 alpha in direct ELISAs.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CCL20
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Neutralization 0.2-1.0 ug/mL
Publications
Read Publications using
AF360 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL20/MIP-3 alpha Antibody [Unconjugated]

  • beta chemokine exodus-1
  • Beta-chemokine exodus-1
  • CC chemokine LARC
  • C-C motif chemokine 20
  • CCL20
  • chemokine (C-C motif) ligand 20
  • CKb4
  • exodus-1
  • LARC
  • LARCLiver and activation-regulated chemokine
  • MIP3 alpha
  • MIP-3 alpha
  • MIP-3a
  • MIP-3-alpha
  • MIP3AMacrophage inflammatory protein 3 alpha
  • SCYA20Small-inducible cytokine A20
  • small inducible cytokine subfamily A (Cys-Cys), member 20
  • ST38

Background

CCL20, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. CCL20 cDNA encodes a 96 amino acid (aa) residue precursor protein with a 26 aa residue signal peptide that is predicted to be cleaved to form the 70 aa residue mature secreted protein. CCL20 is distantly related to other beta chemokines (20 - 28% aa sequence identity) and the gene for CCL20 has been mapped to chromosome 2 rather than 17.

CCL20 has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. The expression of CCL20 is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant CCL20 has been shown to be chemotactic for lymphocytes and to inhibit proliferation of myeloid progenitors in colony formation assays. CCL20 has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors.

  1. Baba, M. et al. (1997) J. Biol. Chem. 272:14893.
  2. Hromas, R. et al. (1997) Blood 89:3315.
  3. Greaves, D.R. et al. (1997) J. Exp. Med. 186:837.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCL20/MIP-3 alpha Antibody (AF360)(23)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 7 applications: Bioassay, ELISA Development, ICC, IHC, IHC-Fr, IHC-P, Neutralization.


Filter By Application
Bioassay
(1)
ELISA Development
(2)
ICC
(3)
IHC
(2)
IHC-Fr
(5)
IHC-P
(5)
Neutralization
(2)
All Applications
Filter By Species
Human
(17)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using AF360 Applications Species
F Parween, SP Singh, HH Zhang, N Kathuria, FA Otaizo-Car, A Shamsaddin, PJ Gardina, S Ganesan, J Kabat, HA Lorenzi, TG Myers, JM Farber Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells bioRxiv : the preprint server for biology, 2023-02-13;0(0):. 2023-02-13 [PMID: 36789428] (ICC, Human) ICC Human
KR Brink, MG Hunt, AM Mu, K Groszman, KV Hoang, KP Lorch, BH Pogostin, JS Gunn, JJ Tabor An E. coli display method for characterization of peptide-sensor kinase interactions Nature Chemical Biology, 2022-12-08;0(0):. 2022-12-08 [PMID: 36482094] (Human) Human
Wang W, Lin Y, Zhang F et al. Single cell transcriptomic analysis of human amnion identifies cell-specific signatures associated with membrane rupture and parturition Cell Biosci. 5/18/2022 [PMID: 35585644]
Louis Boafo Kwantwi, Shujing Wang, Wenjun Zhang, Weidong Peng, Zeyu Cai, Youjing Sheng et al. Tumor-associated neutrophils activated by tumor-derived CCL20 (C-C motif chemokine ligand 20) promote T cell immunosuppression via programmed death-ligand 1 (PD-L1) in breast cancer Bioengineered 9/14/2021 [PMID: 34519637]
JL Booth, ES Duggan, VI Patel, W Wu, DM Burian, DC Hutchings, VL White, KM Coggeshall, MG Dozmorov, JP Metcalf Gene expression profiling of primary human type I alveolar epithelial cells exposed to Bacillus anthracis spores reveals induction of neutrophil and monocyte chemokines Microb. Pathog., 2018-04-25;0(0):. 2018-04-25 [PMID: 29704667] (Neutralization, Human) Neutralization Human
XP Zhang, ZJ Hu, AH Meng, GC Duan, QT Zhao, J Yang Role of CCL20/CCR6 and the ERK signaling pathway in lung adenocarcinoma Oncol Lett, 2017-10-23;14(6):8183-8189. 2017-10-23 [PMID: 29250193] (IHC-P, Human) IHC-P Human
Rump L, Mattey D, Kehoe O, Middleton J An initial investigation into endothelial CC chemokine expression in the human rheumatoid synovium. Cytokine, 2017-09-01;97(0):133-140. 2017-09-01 [PMID: 28648867] (IHC-Fr, Human) IHC-Fr Human
X Ye, R Wang, R Bhattachar, D Boulbes, F Fan, L Xia, H Adoni, NJ Ajami, MC Wong, DP Smith, JF Petrosino, S Venable, W Qiao, V Baladanday, DM Maru, LM Ellis Fusobacterium nucleatum subspecies animalis influences pro-inflammatory cytokine expression and monocyte activation in human colorectal tumors Cancer Prev Res (Phila), 2017-05-08;0(0):. 2017-05-08 [PMID: 28483840] (Neutralization, Human) Neutralization Human
Brand O, Somanath S, Moermans C, Yanagisawa H, Hashimoto M, Cambier S, Markovics J, Bondesson A, Hill A, Jablons D, Wolters P, Lou J, Marks J, Baron J, Nishimura S Transforming Growth Factor-beta and Interleukin-1beta Signaling Pathways Converge on the Chemokine CCL20 Promoter. J Biol Chem, 2015-04-27;290(23):14717-28. 2015-04-27 [PMID: 25918170] (IHC-P, Human) IHC-P Human
Yu , Qing, Lou , Xiang-mi, He , Yan Preferential recruitment of Th17 cells to cervical cancer via CCR6-CCL20 pathway. PLoS ONE, 2015-03-13;10(3):e0120855. 2015-03-13 [PMID: 25768730] (Bioassay, Human) Bioassay Human
Show All 23 Publications.

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Bioinformatics

Gene Symbol CCL20
Uniprot