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CCL20/MIP-3 alpha Antibody [Biotin]

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CCL20/MIP‑3a was detected in immersion fixed paraffin-embedded sections of human lymph node using 15 µg/mL Goat Anti-Human CCL20/MIP‑3a Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF360) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
CCL20/MIP‑3a was detected in immersion fixed paraffin-embedded sections of human lymph node using 15 µg/mL Goat Anti-Human CCL20/MIP‑3a Biotinylated Antigen Affinity-purified Polyclonal Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ELISA(Det)
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Unconjugated
AF360
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF360
Carrier Free
AF360
Carrier Free
AF360-SP
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CCL20/MIP-3 alpha Antibody [Biotin] Summary

Immunogen
E. coli-derived recombinant human CCL20/MIP-3 alpha
Ala27-Met96 (Asn95Asp)
Accession # P78556
Specificity
Detects human CCL20/MIP-3 alpha in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human (rh) MIP‑3 beta , recombinant mouse (rm) MIP‑3 beta , rhMCP-1, rmMIP‑3 alpha , recombinant rat MIP‑3 alpha , and rmJE/MCP-1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CCL20
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Human CCL20/MIP-3 alpha Antibody (Catalog # MAB360)
ELISA Detection:Human CCL20/MIP-3 alpha Biotinylated Antibody (Catalog # BAF360)
Standard: Recombinant Human CCL20/MIP-3 alpha (Catalog # 360-MP)
Readout System
Publications
Read Publications using
BAF360 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL20/MIP-3 alpha Antibody [Biotin]

  • beta chemokine exodus-1
  • Beta-chemokine exodus-1
  • CC chemokine LARC
  • C-C motif chemokine 20
  • CCL20
  • chemokine (C-C motif) ligand 20
  • CKb4
  • exodus-1
  • LARC
  • LARCLiver and activation-regulated chemokine
  • MIP3 alpha
  • MIP-3 alpha
  • MIP-3a
  • MIP-3-alpha
  • MIP3AMacrophage inflammatory protein 3 alpha
  • SCYA20Small-inducible cytokine A20
  • small inducible cytokine subfamily A (Cys-Cys), member 20
  • ST38

Background

CCL20, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. CCL20 cDNA encodes a 96 amino acid (aa) residue precursor protein with a 26 aa residue signal peptide that is predicted to be cleaved to form the 70 aa residue mature secreted protein. CCL20 is distantly related to other beta chemokines (20 - 28% aa sequence identity) and the gene for CCL20 has been mapped to chromosome 2 rather than 17.

CCL20 has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. The expression of CCL20 is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant CCL20 has been shown to be chemotactic for lymphocytes and to inhibit proliferation of myeloid progenitors in colony formation assays. CCL20 has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors.

  1. Baba, M. et al. (1997) J. Biol. Chem. 272:14893.
  2. Hromas, R. et al. (1997) Blood 89:3315.
  3. Greaves, D.R. et al. (1997) J. Exp. Med. 186:837.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CCL20/MIP-3 alpha
Antibody [Biotin]
BAF360
CCL20/MIP-3 alpha Antibody [Biotin]
Species: Hu
Applications: WB, IHC, ELISA(Det)

Publications for CCL20/MIP-3 alpha Antibody (BAF360)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: Antibody Array Development, ELISA Development, IHC, IHC-P.


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Antibody Array Development
(1)
ELISA Development
(1)
IHC
(1)
IHC-P
(1)
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Human
(4)
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Showing Publications 1 - 7 of 7.
Publications using BAF360 Applications Species
Eckfeld C, Schoeps B, H�u�ler D et al. TIMP-1 is a novel ligand of Amyloid Precursor Protein and triggers a proinflammatory phenotype in human monocytes Journal of Cell Biology 2023-02-06 [PMID: 36629908]
Eckfeld C, Schoeps B, H�u�ler D et al. TIMP-1 is a novel ligand of Amyloid Precursor Protein and triggers a proinflammatory phenotype in human monocytes Journal of Cell Biology 2/6/2023 [PMID: 36629908]
Mehdi Bouhaddou, Danish Memon, Bjoern Meyer, Kris M. White, Veronica V. Rezelj, Miguel Correa Marrero et al. The Global Phosphorylation Landscape of SARS-CoV-2 Infection Cell 8/1/2020 [PMID: 32645325]
G Lu, X Zhang, L Shen, Q Qiao, Y Li, J Sun, J Zhang CCL20 secreted from IgA1-stimulated human mesangial cells recruits inflammatory Th17 cells in IgA nephropathy PLoS ONE, 2017-05-26;12(5):e0178352. 2017-05-26 [PMID: 28552941] (IHC, Human) IHC Human
Hu H, Kwun J, Aizenstein BD, Knechtle SJ Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine. Transplantation, 2009-06-27;87(12):1814-20. 2009-06-27 [PMID: 19543058] (Antibody Array Development, Human) Antibody Array Development Human
Lecureuil C, Combadiere B, Mazoyer E, Bonduelle O, Samri A, Autran B, Debre P, Combadiere C Trapping and apoptosis of novel subsets of memory T lymphocytes expressing CCR6 in the spleen of HIV-infected patients. Blood, 2006-12-29;109(9):3649-57. 2006-12-29 [PMID: 17197436] (ELISA Development, Human) ELISA Development Human
Page G, Miossec P Paired synovium and lymph nodes from rheumatoid arthritis patients differ in dendritic cell and chemokine expression. J. Pathol., 2004-09-01;204(1):28-38. 2004-09-01 [PMID: 15307135] (IHC-P, Human) IHC-P Human

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Bioinformatics

Gene Symbol CCL20
Uniprot