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CCL2/MCP1 Antibody - BSA Free

 (3 Publications)
 (1 Review)
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Western Blot: CCL2/MCP1 Antibody [NBP1-07034] - Analysis of recombinant mouse MCP1. Image provided via product review by verified customer.
Western Blot: CCL2/MCP1 Antibody [NBP1-07034] - Analysis of recombinant mouse MCP1. Image provided via product review by verified customer.
Western Blot: CCL2/MCP1 Antibody [NBP1-07034] - Analysis of MCP1 in Hela whole cell lysates.

Product Details

Summary
Reactivity Hu, Rt, MuSpecies Glossary
Applications WB, ICC/IF (-), IHC (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.42 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-07034AF350
Alexa Fluor 405
NBP1-07034AF405
Alexa Fluor 488
NBP1-07034AF488
Alexa Fluor 532
NBP1-07034AF532
Alexa Fluor 594
NBP1-07034AF594
Alexa Fluor 647
NBP1-07034AF647
Alexa Fluor 700
NBP1-07034AF700
Alexa Fluor 750
NBP1-07034AF750
Allophycocyanin
NBP1-07034APC
Biotin
NBP1-07034B
DyLight 350
NBP1-07034UV
DyLight 405
NBP1-07034V
DyLight 488
NBP1-07034G
DyLight 550
NBP1-07034R
DyLight 594
NBP1-07034DL594
DyLight 650
NBP1-07034C
DyLight 680
NBP1-07034FR
DyLight 755
NBP1-07034IR
FITC
NBP1-07034F
HRP
NBP1-07034H
Janelia Fluor 549
NBP1-07034JF549
Janelia Fluor 646
NBP1-07034JF646
PE
NBP1-07034PE
PerCP
NBP1-07034PCP
mFluor Violet 450 SE
NBP1-07034MFV450
mFluor Violet 500 SE
NBP1-07034MFV500
mFluor Violet 610 SE
NBP1-07034MFV610
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP1-07034SS
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CCL2/MCP1 Antibody - BSA Free Summary

Immunogen
Synthetic peptide made to an internal portion of rat MCP1 (within residues 30-80). [Swiss-Prot# P14844]
Localization
Secreted
Predicted Species
Mouse (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CCL2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/ml
Application Notes
In Western blot, a band is seen at ~16 kDa. This antibody did not work in IHC with mouse tissue or in ICC.
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
CCL2/MCP1 Overexpression Lysate
Control Peptide
CCL2/MCP1 Peptide (NBP1-07034PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-07034 in the following applications:
Publications
Read Publications using
NBP1-07034 in the following applications:

  • WB
    2 publications

Reactivity Notes

Mouse reactivity reported by a customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1.42 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CCL2/MCP1 Antibody - BSA Free

  • C-C motif chemokine 2
  • chemokine (C-C motif) ligand 2
  • GDCF-2
  • HC11Monocyte secretory protein JE
  • MCAFMonocyte chemotactic and activating factor
  • MCP1 Monocyte chemotactic protein 1
  • MCP-1 Small-inducible cytokine A2
  • MGC9434
  • Monocyte chemoattractant protein 1
  • monocyte chemoattractant protein-1
  • SCYA2HSMCR30
  • small inducible cytokine A2 (monocyte chemotactic protein 1, homologous tomouse Sig-je)
  • small inducible cytokine subfamily A (Cys-Cys), member 2
  • SMC-CF

Background

MCP-1 (monocyte chemoattracctant protein-1, also called C-C motif chemokine 2 or CCL2) is a secreted protein belonging to intercrine beta (chemokine CC) family and it specifically act as a chemotactic factor to attracts monocytes, CD4+ T cells, NK cells as well as for CCR2 expressing cells, but not neutrophils. Both hematopoietic and non-hematopoietic cells that include monocytes/macrophages, dendritic cells, astrocytes, endothelial cells and fibroblasts express MCP-1, and monocytes/macrophages are the major source of MCP-1 in peripheral blood. Besides, expression at acute inflammation sites, MCP-1 is upregulated in chronic inflammation, including autoimmune disease, atherosclerosis, cancer, and chronic infection. MCP-1 has been implicated in both pro-inflammatory and anti-inflammatory responses, and is a potential intervention point for the treatment of various inflammatory/autoimmune diseases (multiple sclerosis, atherosclerosis, insulin-resistant diabetes, rheumatoid arthritis and lupus nephritis), cancers (breast and prostate cancer) and HIV-1/AIDS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CCL2/MCP1 Antibody
- BSA Free
NBP1-07034
CCL2/MCP1 Antibody - BSA Free
Species: Hu, Rt, Mu
Applications: WB, ICC/IF (-), IHC (-)

Publications for CCL2/MCP1 Antibody (NBP1-07034)(3)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 1 application: WB.


Filter By Application
WB
(2)
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Human
(1)
Rat
(1)
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Review for CCL2/MCP1 Antibody (NBP1-07034) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-07034:
Filter by Applications
WB
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot CCL2/MCP1 NBP1-07034
Enlarge 		5
reviewed by:
David Jeng
 WB Mouse 06/07/2013
View

Summary

ApplicationWestern Blot
Sample TestedRecombinant Mouse MPC1 protein
SpeciesMouse

Product General Protocols

View specific protocols for CCL2/MCP1 Antibody (NBP1-07034): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CCL2/MCP1 Antibody (NBP1-07034). (Showing 1 - 2 of 2 FAQs).

  1. Our customer would like to perform IHC with samples from Rat. She has questions regarding antibodies of choice: 1. Is it possible to use NB400-142 for IHC? 2. I am having difficulty of choosing between NBP1-77679 and NBP1-77680. Would you please help suggest? 3. Although it is described as IHC (-) on the product page of NBP1-07034, This antibody did not work in IHC with mouse tissue is also described. How about Rat samples? Is it possible that the antibody can work in IHC with rat tissues?
    • To answer your questions about NB400-142, this antibody has not been validated for IHC and we cannot guarantee that it will work. Both NBP1-77679 and NBP1-77680 have been validated for use in rat and IHC. The difference between these 2 antibodies is the immunogen as they were raised against different regions of the protein. Please point out the immunogen to your customer and have them decide what would be best for their research. NBP1-07034 does not work in IHC, it is only guaranteed for Western blot in human, mouse and rat. When an application is followed by a (-) I would not recommend using this application in any species.
  2. Would you please let me know what buffer I should use for WB with antibody (MCP1 Antibody)?
    • In western blot, we recommend diluting this antibody in 1% non-fat dry milk in TBST (0.1% tween-20). This is the same diluent we use for our secondary antibody. For blocking, we use 5% non-fat dry milk in TBST for 1 hour at room temperature.

Control Lysate(s)

CCL2/MCP1 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Research Areas for CCL2/MCP1 Antibody (NBP1-07034)

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Blogs on CCL2/MCP1.

Check out the latest blog posts on CCL2/MCP1.
MCP-1 - chemoattractant protein involed in monocyte migration and infiltration
Monocyte chemotractant protein-1 (MCP-1), also known as CCL2, is a key chemokine involved in the migration of monocytes and macrophages to sites of active inflammation. It is a member of the C-C/beta family of cytokines, characterized by the Cys-C...  Read full blog post.
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Contact Information

Product PDFs

  NBP1-07034 Specific Protocol
  SDS
  PDF Datasheet
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Recent Reviews

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Western Blot CCL2/MCP1 NBP1-07034
Enlarge
 David Jeng
06/07/2013
Application: WB
Species: Mouse
read this review

Bioinformatics

Gene Symbol CCL2