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CCL2/JE/MCP-1 Antibody [Unconjugated]

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Western blot shows 25 ng of Recombinant Mouse CCL2/JE/MCP-1 (479-JE), Recombinant Human CCL2/JE/MCP-1 (Catalog # 279-MC), Recombinant Rat CCL2/JE/MCP 3144-JE), Recombinant Mouse CCL11/Eotaxin (Catalog # 420-ME), ...read more
Recombinant Mouse CCL2/JE (Catalog # 479-JE) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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CCL2/JE/MCP-1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse CCL2/JE/MCP-1
Gln24-Arg96
Accession # Q5SVU3
Specificity
Detects mouse CCL2/JE/MCP-1 in ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
Ccl2
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.5-2.5 ug/mL
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Mouse CCL2/JE/MCP-1 Antibody (Catalog # AF-479-NA)
ELISA Detection:Mouse CCL2/JE/MCP-1 Biotinylated Antibody (Catalog # BAF479)
Standard: Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE)
Reviewed Applications
Read 2 Reviews rated 4
using
AF-479-NA in the following applications:

Publications
Read Publications using
AF-479-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL2/JE/MCP-1 Antibody [Unconjugated]

  • C-C motif chemokine ligand 2
  • CCL2
  • GDCF-2
  • HC11
  • HSMCR30
  • MCAF
  • Mcp1
  • MCP-1
  • SCYA2
  • SMC-CF

Background

Mouse CCL2 is a member of the beta (C-C) subfamily of chemokines. The murine CCL2 gene was initially identified as a platelet-derived growth factor-inducible gene in murine fibroblasts. Mouse CCL2 cDNA encodes a 148 amino acid (aa) residue with a putative 23 aa signal peptide that is cleaved to generate the mature protein. Mouse CCL2 shares 82% amino acid sequence identity with rat CCL2. Mouse CCL2 also shares 55% amino acid sequence identity with human MCP-1. Compared to human MCP-1, mouse CCL2 has a 49 aa residue extension at the carboxy-terminus. Mouse CCL2 has full activity on human cells while human MCP-1 has limited activity on mouse cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCL2/JE/MCP-1 Antibody (AF-479-NA)(58)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 12 applications: Bioassay, Dot Blot, ELISA Development, ELISA Development (Capture), Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Luminex Development, Neutralization, Western Blot.


Filter By Application
Bioassay
(1)
Dot Blot
(1)
ELISA Development
(10)
ELISA Development (Capture)
(1)
Flow Cytometry
(1)
ICC
(4)
IHC
(5)
IHC-Fr
(5)
IHC-P
(4)
Luminex Development
(1)
Neutralization
(16)
Western Blot
(6)
All Applications
Filter By Species
Human
(4)
Mouse
(42)
Rat
(2)
All Species
Showing Publications 1 - 10 of 58. Show All 58 Publications.
Publications using AF-479-NA Applications Species
de Souza, S;Rosario Claudio, J;Sim, J;Inyang, KE;Dagenais, A;Monahan, K;Lee, B;Ramakrishnan, H;Parmar, V;Geron, M;Scherrer, G;Folger, JK;Laumet, G; Interleukin-10 signaling in somatosensory neurons controls CCL2 release and inflammatory response Brain, behavior, and immunity 2023-12-09 [PMID: 38081433] (IHC, Mouse) IHC Mouse
Hong, J;Son, M;Sin, J;Kim, H;Chung, DK; Nanoparticles of Lactiplantibacillus plantarum K8 Reduce Staphylococcus aureus Respiratory Infection and Tumor Necrosis Factor Alpha- and Interferon Gamma-Induced Lung Inflammation Nutrients 2023-11-09 [PMID: 38004123] (Mouse) Mouse
Q Ye, J Jo, CY Wang, H Oh, TJ Choy, K Kim, A Dâ Alessan, YK Reshetnyak, SY Jung, Z Chen, SP Marrelli, HK Lee Astrocytic Slc4a4 regulates blood-brain barrier integrity in healthy and stroke brains via a NO-CCL2-CCR2 pathway bioRxiv : the preprint server for biology, 2023-04-03;0(0):. 2023-04-03 [PMID: 37066295] (IHC, Neutralization, Mouse) IHC, Neutralization Mouse
T Komori, Y Morikawa Essential roles of the cytokine oncostatin M in crosstalk between muscle fibers and immune cells in skeletal muscle after aerobic exercise The Journal of Biological Chemistry, 2022-11-09;0(0):102686. 2022-11-09 [PMID: 36370846] (Western Blot, Mouse) Western Blot Mouse
Hinami Asano, Sanae Hasegawa-Ishii, Ken Arae, Aki Obara, Geoffroy Laumet, Robert Dantzer et al. Infiltration of peripheral immune cells into the olfactory bulb in a mouse model of acute nasal inflammation Journal of Neuroimmunology 7/1/2022 [PMID: 35661951]
Madathilparambil S, Yalamanchili G, Rao T et al. Hypoxia Inducible Factor (HIF) 1 alpha induced regulation of lung injury in pulmonary aspiration is mediated through NF kappa B FASEB Bioadv 5/6/2022 [PMID: 35520392]
L Wang, J Lan, J Tang, N Luo MCP-1 targeting: Shutting off an engine for tumor development Oncology Letters, 2021-11-19;23(1):26. 11/19/2021 [PMID: 34868363]
Alicia Bellomo, Isabelle Mondor, Lionel Spinelli, Marine Lagueyrie, Benjamin J. Stewart, Nicolas Brouilly et al. Reticular Fibroblasts Expressing the Transcription Factor WT1 Define a Stromal Niche that Maintains and Replenishes Splenic Red Pulp Macrophages Immunity 7/1/2020 [PMID: 32562599]
A Sui, X Chen, AM Demetriade, J Shen, Y Cai, Y Yao, Y Yao, Y Zhu, X Shen, B Xie Inhibiting NF-&amp;kappaB Signaling Activation Reduces Retinal Neovascularization by Promoting a Polarization Shift in Macrophages Invest. Ophthalmol. Vis. Sci., 2020-06-03;61(6):4. 2020-06-03 [PMID: 32492108] (Western Blot, Mouse) Western Blot Mouse
Xian Zhang, Jie Li, Songyuan Luo, Minjie Wang, Qin Huang, Zhiyong Deng et al. IgE Contributes to Atherosclerosis and Obesity by Affecting Macrophage Polarization, Macrophage Protein Network, and Foam Cell Formation Arteriosclerosis, Thrombosis, and Vascular Biology 3/1/2020 [PMID: 31996021]
Show All 58 Publications.

Reviews for CCL2/JE/MCP-1 Antibody (AF-479-NA) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using AF-479-NA:
Filter by Applications
IHC-P
(1)
IHC-Fr
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
IHC-P Mouse 01/08/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 23746482
SpeciesMouse
  4
reviewed by:
Verified Customer
IHC-Fr Mouse 01/08/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 23748430
SpeciesMouse

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

 
Verified Customer
01/08/2015
Application: IHC-P
Species: Mouse

 
Verified Customer
01/08/2015
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol Ccl2
Uniprot