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CCL19/MIP-3 beta Antibody (87102) [Unconjugated]

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Recombinant Mouse CCL19/MIP‑3 beta (Catalog # 440-M3) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL19/MIP‑3 beta (50 ng/mL) is neutralized (green line) by increasing concentrationsof Rat Anti‑Mouse CCL19/MIP‑3 beta Monoclonal Antibody (Catalog # MAB880). The ND50 is typically 3-15 µg/mL.
Recombinant Mouse CCL19/MIP‑3 beta (Catalog # 440-M3) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N, ELISA(Cap)
Clone
87102
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB880-SP
Carrier Free
MAB880-500
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CCL19/MIP-3 beta Antibody (87102) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse CCL19/MIP-3 beta
Gly26-Val107-Leu-Glu (Ser108LeuGlu)
Accession # Q548P0
Specificity
Detects mouse CCL19/MIP‑3 beta  in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 28, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 9/10, 11, 12, 17, 20, 21, 22, 24, 25, 27 or recombinant rat CCL20 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
Ccl19
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 3-15 ug/mL
  • Western Blot 1 ug/mL
Application Notes
ELISA Detection: Mouse CCL19/MIP-3beta Biotinylated Antibody (Catalog number BAF880)
Standard: Recombinant Mouse CCL19/MIP-3beta (Catalog number 440-M3)
Publications
Read Publications using
MAB880 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL19/MIP-3 beta Antibody (87102) [Unconjugated]

  • beta chemokine exodus-3
  • Beta-chemokine exodus-3
  • CC chemokine ligand 19
  • C-C motif chemokine 19
  • CCL19
  • chemokine (C-C motif) ligand 19
  • CKb11
  • EBI1-ligand chemokine
  • ELC
  • ELCMIP-3-beta
  • Epstein-Barr virus-induced molecule 1 ligand chemokine
  • exodus-3
  • Macrophage inflammatory protein 3 beta
  • macrophage inflammatory protein 3-beta
  • MGC34433
  • MIP3 beta
  • MIP-3 beta
  • MIP-3b
  • MIP3BCK beta-11
  • SCYA19EBI1 ligand chemokine
  • small inducible cytokine subfamily A (Cys-Cys), member 19
  • Small-inducible cytokine A19

Background

CCL19/MIP-3 beta , also known as ELC (EBI1-Ligand Chemokine), is a beta  chemokine that binds specifically to the chemokine receptor CCR7/EBI-1/BLR-2. Mouse (human) CCL19 cDNA encodes a 108 (98) amino acid precursor protein with a predicted 25 (21) aa signal peptide that is cleaved to form the 83 (77) aa mature secreted protein. CCL19 is distantly related to other beta chemokines (20 - 30% aa sequence identity). Mouse CCL19 shares 83% aa sequence homology with human CCL19. CCL19 has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix, and spleen) in dendritic cells within the T-cell zone. The expression of CCL19 is down-regulated by the anti-inflammatory cytokine IL-10. Recombinant CCL19 has been shown to be chemotactic for T-cells and B-cells. The CCL19 receptor (CCR-7/EBI-1/BLR-2) is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR7 is also strongly up‑regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.

  1. Kim, C.H. et al. (1998) J. Immunol. 160:2418.
  2. Ngo, V.N. et al. (1998) J. Exp. Med. 188:181.
  3. Rossi, D.L. et al. (1997) J. Immunol. 158:1033.
  4. Yoshida, R. et al. (1997) J. Biol. Chem. 272:13803.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCL19/MIP-3 beta Antibody (MAB880)(3)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: ELISA Development (Capture), IHC.


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(1)
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Bioinformatics

Gene Symbol Ccl19
Uniprot