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CCL19/MIP-3 beta Antibody (54909) [Unconjugated]

 (14 Publications)
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CCL19/MIP-3 beta was detected in immersion fixed paraffin-embedded sections of human tonsil using 25 µg/mL Mouse Anti-Human CCL19/ MIP-3 beta Monoclonal Antibody (Catalog # MAB361) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
CCL19/MIP-3 beta was detected in immersion fixed paraffin-embedded sections of human tonsil using 25 µg/mL Mouse Anti-Human CCL19/ MIP-3 beta Monoclonal Antibody (Catalog # MAB361) overnight at 4 °C. Tissue was ...read more
CCL19/MIP-3 beta was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Mouse Anti-Human CCL19/MIP-3 beta Monoclonal Anti-body (Catalog # MAB361) for 3 hours at room ...read more
Human monocyte-derived dendritic cells were stained with Mouse Anti-Human CCL19/MIP‑3 beta Monoclonal Antibody (Catalog # MAB361, filled histogram) or isotype control antibody (Catalog # MAB0041, open ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, Flow, ICC/IF
Clone
54909
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC361U-100UG
Alexa Fluor 405
IC361V-100UG
Alexa Fluor 488
IC361G-100UG
Alexa Fluor 532
FAB361X
Alexa Fluor 594
IC361T-100UG
Alexa Fluor 647
IC361R-100UG
Alexa Fluor 700
IC361N-100UG
Alexa Fluor 750
IC361S-100UG
Allophycocyanin
FAB361A
Biotin
FAB361B
CoraFluor 1
FAB361CL1
DyLight 350
FAB361D
DyLight 405
FAB361E
DyLight 488
FAB361K
DyLight 550
FAB361L
DyLight 594
FAB361M
DyLight 650
FAB361W
DyLight 680
FAB361Y
DyLight 755
FAB361Z
FITC
FAB361F
HRP
FAB361H
Janelia Fluor 525
FAB361JF525
Janelia Fluor 549
FAB361I
Janelia Fluor 585
FAB361JF585
Janelia Fluor 635
FAB361JF635
Janelia Fluor 646
FAB361J
Janelia Fluor 669
FAB361JF669
PerCP
FAB361C
mFluor Violet 450 SE
FAB361MFV450
mFluor Violet 500 SE
FAB361MFV500
mFluor Violet 610 SE
FAB361MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB361-SP
Carrier Free
IC361G-100UG
Carrier Free
IC361V-100UG
Carrier Free
IC361U-100UG
Carrier Free
IC361T-100UG
Carrier Free
IC361S-100UG
Carrier Free
IC361R-100UG
Carrier Free
IC361N-100UG
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CCL19/MIP-3 beta Antibody (54909) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human CCL19/MIP-3 beta
Gly22-Ser98
Accession # Q99731.1
Specificity
Detects human CCL19/MIP-3 beta in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 20, 21, 22, 23, 24, 25, recombinant mouse CCL2, 3, 4, 5, 6, 7, 9, 11, 12, 21, 22, or 25 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CCL19
Purity Statement
Protein A or G purified from ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB361 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in Tris and Acetic Acid. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL19/MIP-3 beta Antibody (54909) [Unconjugated]

  • beta chemokine exodus-3
  • Beta-chemokine exodus-3
  • CC chemokine ligand 19
  • C-C motif chemokine 19
  • CCL19
  • chemokine (C-C motif) ligand 19
  • CKb11
  • EBI1-ligand chemokine
  • ELC
  • ELCMIP-3-beta
  • Epstein-Barr virus-induced molecule 1 ligand chemokine
  • exodus-3
  • Macrophage inflammatory protein 3 beta
  • macrophage inflammatory protein 3-beta
  • MGC34433
  • MIP3 beta
  • MIP-3 beta
  • MIP-3b
  • MIP3BCK beta-11
  • SCYA19EBI1 ligand chemokine
  • small inducible cytokine subfamily A (Cys-Cys), member 19
  • Small-inducible cytokine A19

Background

CCL19, also known as MIP-3 beta and ELC (EBI1-Ligand Chemokine), is a 77 amino acid (aa) beta chemokine that is distantly related to other beta chemokines (20-30% aa sequence identity). The gene for MIP-3 beta has been mapped to chromosome 9p13 rather than chromosome 17 where the genes for many human beta chemokines are clustered. MIP-3 beta is constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix and spleen). The expression of MIP-3 beta is
down‑regulated by the anti-inflammatory cytokine IL-10. Recombinant MIP-3 beta is chemotactic for cultured human lymphocytes. MIP-3 beta is a ligand for CCR7 (previously referred to as the Epstein-Barr virus-induced gene 1 (EBI1) orphan receptor), a chemokine receptor that is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR7 is strongly up-regulated in B cells infected with Epstein-Barr virus and T cells infected with herpesvirus 6 or 7.

  1. Yoshida, R. et al. (1997) J. Biol. Chem. 272:13803.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCL19/MIP-3 beta Antibody (MAB361)(14)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 6 applications: ELISA Development, Flow Cytometry, ICC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
ELISA Development
(1)
Flow Cytometry
(2)
ICC
(2)
IHC-Fr
(2)
IHC-P
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(7)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using MAB361 Applications Species
Hussong, SA;Banh, AQ;Van Skike, CE;Dorigatti, AO;Hernandez, SF;Hart, MJ;Ferran, B;Makhlouf, H;Gaczynska, M;Osmulski, PA;McAllen, SA;Dineley, KT;Ungvari, Z;Perez, VI;Kayed, R;Galvan, V; Soluble pathogenic tau enters brain vascular endothelial cells and drives cellular senescence and brain microvascular dysfunction in a mouse model of tauopathy Nature communications 4/25/2023 [PMID: 37185259]
Hussong, SA;Banh, AQ;Van Skike, CE;Dorigatti, AO;Hernandez, SF;Hart, MJ;Ferran, B;Makhlouf, H;Gaczynska, M;Osmulski, PA;McAllen, SA;Dineley, KT;Ungvari, Z;Perez, VI;Kayed, R;Galvan, V; Soluble pathogenic tau enters brain vascular endothelial cells and drives cellular senescence and brain microvascular dysfunction in a mouse model of tauopathy Nature communications 2023-04-25 [PMID: 37185259]
Thomas Krausgruber, Anna Redl, Daniele Barreca, Konstantin Doberer, Daria Romanovskaia, Lina Dobnikar et al. Single-cell and spatial transcriptomics reveal aberrant lymphoid developmental programs driving granuloma formation Immunity 2/14/2023 [PMID: 36750099]
M Artinger, C Matti, OJ Gerken, CT Veldkamp, DF Legler A Versatile Toolkit for Semi-Automated Production of Fluorescent Chemokines to Study CCR7 Expression and Functions International Journal of Molecular Sciences, 2021-04-16;22(8):. 2021-04-16 [PMID: 33923834] (Flow Cytometry, Western Blot, Human) Flow Cytometry, Western Blot Human
Kinchen J, Chen HH, Parikh K et al. Structural Remodeling of the Human Colonic Mesenchyme in Inflammatory Bowel Disease. Cell 9/25/2018 [PMID: 30270042]
Rump L, Mattey D, Kehoe O, Middleton J An initial investigation into endothelial CC chemokine expression in the human rheumatoid synovium. Cytokine, 2017-09-01;97(0):133-140. 2017-09-01 [PMID: 28648867] (IHC-Fr, Human) IHC-Fr Human
Xiaozhi Xiaozhi, Liang Liang, Liping Liping, Zhao Rong, Yanhu Yanhu, Xiangqing Xiangqing CC-chemokine receptor 7 and its ligand CCL19 promote mitral valve interstitial cell migration and repair The Journal of Biomedical Research 9/9/2015 [PMID: 26668580]
Kudo-Saito C, Yura M, Yamamoto R, Kawakami Y Induction of immunoregulatory CD271+ cells by metastatic tumor cells that express human endogenous retrovirus H. Cancer Res, 2014-03-01;74(5):1361-70. 2014-03-01 [PMID: 24590808] (IHC-P, Human) IHC-P Human
Pinto S, Martinez-Romero A, O'Connor J, Gil-Benso R, San-Miguel T, Terradez L, Monteagudo C, Callaghan R Intracellular coexpression of CXC- and CC- chemokine receptors and their ligands in human melanoma cell lines and dynamic variations after xenotransplantation. BMC Cancer, 2014-02-22;14(0):118. 2014-02-22 [PMID: 24559071] (Flow Cytometry, ICC, Human) Flow Cytometry, ICC Human
Rebecca C. Harris, Kimberly A. Chianese-Bullock, Gina R. Petroni, Jochen T. Schaefer, Louis B. Brill, Kerrington R. Molhoek et al. The Vaccine-site Microenvironment Induced by Injection of Incomplete Freund's Adjuvant, With or Without Melanoma Peptides Journal of Immunotherapy 1/1/2012 [PMID: 22130163]
Show All 14 Publications.

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Bioinformatics

Gene Symbol CCL19
Uniprot