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Cathepsin L Antibody (193720)

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Product Details

Summary
Reactivity ISpecies Glossary
Applications WB, IP
Clone
193720
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Cathepsin L Antibody (193720) Summary

Immunogen
S. frugiperda ovarian cell line Sf 21-derived Insect Cathepsin L
Specificity
Detects pro form of Insect Cathepsin L in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) Cathepsin S is observed and no cross-reactivity with rhCathepsin B, F, K, L2/V, and activated, mature insect Cathepsin L is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CTSL1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB2259 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin L Antibody (193720)

  • Cathepsin L
  • cathepsin L1
  • CATL
  • CTSL
  • CTSL1
  • CTSLEC 3.4.22.15
  • EC 3.4.22
  • FLJ31037
  • Major excreted protein
  • MEP

Background

The pro form of insect cell Cathepsin L is stable at neutral pH, but autoactivates in acidic buffers under reducing conditions. The activated enzyme is similar in substrate specificity to mammalian Cathepsin L, and is very active as a protease, as judged by its ability to degrade azocasein. Cystatins A, B, C, E/M, F, SA, and SN are potent inhibitors of the insect cell Cathepsin L. Like other cysteine proteases related to papain, insect cell Cathepsin L is also inhibited by E-64 and reagents which react with thiols.
  1. Johnson, G.D. and W. Jiang (2005) Arch. Biochem. Biophys.444:7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cathepsin L Antibody (MAB2259)(2)

We have publications tested in 2 confirmed species: Drosophila, Insect.

We have publications tested in 2 applications: Immunoprecipitation, Western Blot.


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Immunoprecipitation
(1)
Western Blot
(2)
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Drosophila
(1)
Insect
(1)
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Bioinformatics

Gene Symbol CTSL1