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Cathepsin B Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Cathepsin B Antibody [NBP1-86048] - Staining in human thyroid gland and skeletal muscle tissues . Corresponding CTSB RNA-seq data are presented for the same ...read more
Immunocytochemistry/ Immunofluorescence: Cathepsin B Antibody [NBP1-86048] - Staining of human cell line A-431 shows localization to nucleoli & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Cathepsin B Antibody [NBP1-86048] - Staining of human thyroid gland shows moderate granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Cathepsin B Antibody [NBP1-86048] - Staining of human cerebral cortex shows granular cytoplasmic positivity in neuronal cells.
Immunohistochemistry-Paraffin: Cathepsin B Antibody [NBP1-86048] - Staining of human lung shows cytoplasmic positivity in macrophages.
Immunohistochemistry-Paraffin: Cathepsin B Antibody [NBP1-86048] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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Cathepsin B Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DELVNYVNKRNTTWQAGHNFYNVDMSYLKRLCGTFLGGPKPPQRVMFTEDLKLPASFDAREQWPQCPTIKEIRDQGSCGSCWAFGAVEAISDRICIHTNAHVSVEVSAEDLLTCCGSMCGDGCNGGYPA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CTSB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: PFA/Triton X-100
Control Peptide
Cathepsin B Protein (NBP1-86048PEP)
Publications
Read Publications using NBP1-86048.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 22918166)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Cathepsin B Antibody

  • APP secretase
  • APPS
  • Cathepsin B
  • Cathepsin B1
  • CPSBamyloid precursor protein secretase
  • CTSB
  • cysteine protease
  • EC 3.4.22
  • EC 3.4.22.1

Background

The cathepsin family of proteolytic enzymes contains several diverse classes of proteases. The cysteine protease class comprises cathepsins B, L, H, K, S and O. The aspartyl protease class is composed of cathepsins D and E. cathepsin G is in the serine protease class. Most cathepsins are lysosomal and each is involved in cellular metabolism, participating in various events such as peptide biosynthesis and protein degradation. cathepsin B is expressed in luminal epithelial cells, indicating that cathepsin B is a marker for secretory cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cathepsin B Antibody (NBP1-86048)(2)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Research Areas for Cathepsin B Antibody (NBP1-86048)

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Blogs on Cathepsin B.

Cathepsin B - a lysosomal protease with potential of an important drug target in neurological diseases and cancer
Cathepsins are a family of lysosomal proteases (serine, aspartic and cysteine proteases) that acts in conjunction with lipases and nucleases to degrade biological macromolecules in the lysosomes (1). While most cathepsins are ubiquitously expressed...  Read full blog post.

Caspase 11: A novel non-canonical inflammasomes
Cell death via apoptosis is a key cellular function triggered by the cell death receptor family and their ligands. This regulated process then transmits downstream signals through adaptor molecules ending with the caspase cysteine proteases. Caspas...  Read full blog post.

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Bioinformatics

Gene Symbol CTSB