Novus Biologicals products are now on bio-techne.com

Carboxypeptidase B1/CPB1 Antibody

Images

 
Western Blot: Carboxypeptidase B1/CPB1 Antibody [NBP2-15698] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Carboxypeptidase ...read more
Immunohistochemistry-Paraffin: Carboxypeptidase B1/CPB1 Antibody [NBP2-15698] - Paraffin-embedded rat pancreas. Carboxypeptidase B antibody [N3C3] diluted at 1:1000.
Western Blot: Carboxypeptidase B1/CPB1 Antibody [NBP2-15698] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Carboxypeptidase B antibody [N3C3] diluted at 1:50000. The ...read more
Western Blot: Carboxypeptidase B1/CPB1 Antibody [NBP2-15698] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Carboxypeptidase B antibody [N3C3] diluted at 1:1000. ...read more
Western Blot: Carboxypeptidase B1/CPB1 Antibody [NBP2-15698] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Carboxypeptidase B antibody [N3C3] diluted at 1:50000. The ...read more
Immunohistochemistry-Paraffin: Carboxypeptidase B1/CPB1 Antibody [NBP2-15698] - Paraffin-embedded mouse pancreas. Carboxypeptidase B antibody [N3C3] diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Carboxypeptidase B1/CPB1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Carboxypeptidase B1/CPB1. The exact sequence is proprietary.
Localization
Secreted
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CPB1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Canine (86%), Porcine (85%), Bovine (83%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for Carboxypeptidase B1/CPB1 Antibody

  • carboxypeptidase B
  • carboxypeptidase B1 (tissue)
  • Carboxypeptidase B1
  • CPB
  • CPB1
  • DKFZp779K1333
  • EC 3.4.17
  • EC 3.4.17.2
  • Pancreas-specific protein
  • PASP
  • PCPB
  • procarboxypeptidase B

Background

Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated. The B1 and B2 forms differ from each other mainly in isoelectric point. Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. It is not elevated in pancreatic carcinoma. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

M6000B
Species: Mu
Applications: ELISA
DY417
Species: Mu
Applications: ELISA
NBP2-92630
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
MAB208
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, ICFlow, Neut, Simple Western, WB
DY1707
Species: Hu
Applications: ELISA
NB600-930
Species: Hu, Rt
Applications: ELISA, WB
AF6036
Species: Hu
Applications: IHC, WB
MAB1455
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
NBP1-58268
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NBP2-52559
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, IHC, IHC-P, WB
NB600-533
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IP, Simple Western, WB
H00000072-D01P
Species: Hu
Applications: WB
NBP1-32660
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-89278
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
DY4517-05
Species: Mu
Applications: ELISA
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
AF1267
Species: Hu
Applications: IP, Neut, WB
NBP1-92172
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
AF3667
Species: Hu, Mu
Applications: Dual ISH-IHC, ICC, IHC, Simple Western, WB

Publications for Carboxypeptidase B1/CPB1 Antibody (NBP2-15698) (0)

There are no publications for Carboxypeptidase B1/CPB1 Antibody (NBP2-15698).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Carboxypeptidase B1/CPB1 Antibody (NBP2-15698) (0)

There are no reviews for Carboxypeptidase B1/CPB1 Antibody (NBP2-15698). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Carboxypeptidase B1/CPB1 Antibody (NBP2-15698). (Showing 1 - 2 of 2 FAQ).

  1. I use porcine carboxypeptidase B (CPB) in my fermentation process. I want to detect the residual CPB in my finished product preferably by ELISA/western blot. How will antibody pairs from Novus Biologics help me do that? Is a colorimetric assay possible with the antibody pair?
    • Any detection system can be employed when using the antibody pairs. The detection system will depend on the secondary used against the detection primary antibody from the pair, and the conjugate on the secondary antibody. The pair will work effectively in capturing the protein of interest, and subsequently detecting it with the second antibody in the pair for ELISA. This pair is not evaluated in Western Blot directly, but there Novus carries similar primary antibodies that we have that are validated in that application.
  2. I am trying to establish ELISA detection for carboxy peptidase. I am using a rat recombinant carboxypeptodase b from Roche diagnostics (cat no.03358682103). Can you please suggest the suitable kit .
    • Here is a link to our CPB1 products. H00001360-AP21 and H00001360-AP11 may suit your needs.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Carboxypeptidase B1/CPB1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol CPB1
Uniprot