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ACOT7 Antibody

Images

 
Independent Antibodies: Western Blot: ACOT7 Antibody [NBP1-89278] - Analysis using Anti-ACOT7 antibody NBP1-89278 (A) shows similar pattern to independent antibody NBP1-89279 (B).
Immunocytochemistry/ Immunofluorescence: ACOT7 Antibody [NBP1-89278] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: ACOT7 Antibody [NBP1-89278] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Independent Antibodies: Immunohistochemistry-Paraffin: ACOT7 Antibody [NBP1-89278] - Staining of human cerebellum, cerebral cortex, kidney and pancreas using Anti-ACOT7 antibody NBP1-89278 (A) shows similar ...read more
Orthogonal Strategies: Immunohistochemistry-Paraffin: ACOT7 Antibody [NBP1-89278] - Analysis in human cerebral cortex and pancreas tissues. Corresponding ACOT7 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: ACOT7 Antibody [NBP1-89278] - Staining of human kidney shows moderate to strong granular cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: ACOT7 Antibody [NBP1-89278] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: ACOT7 Antibody [NBP1-89278] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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ACOT7 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: RLCEFGRQASSRRLVAGQGCVGPRRGCCAPVQVVGPRADLPPCGACITGRIMRPDDANVAGNVHGGTILKMIEEAGAIISTRHCNSQNGE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACOT7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
ACOT7 Protein (NBP1-89278PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ACOT7 Antibody

  • ACH1
  • acyl-CoA thioesterase 7CTE-IIa
  • BACHEC 3.1.2.2
  • brain acyl CoA hydrolase
  • Brain acyl-CoA hydrolase
  • CTE-IILACH
  • cytosolic acyl coenzyme A thioester hydrolase
  • hBACH
  • Long chain acyl-CoA thioester hydrolase
  • long chain

Background

The ACOT7 gene encodes a member of the acyl coenzyme family. The encoded protein hydrolyzes the CoA thioester ofpalmitoyl-CoA and other long-chain fatty acids. Decreased expression of this gene may be associated with mesialtemporal lobe epilepsy. Alternatively

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ACOT7
Entrez
Uniprot