I use porcine carboxypeptidase B (CPB) in my fermentation process. I want to detect the residual CPB in my finished product preferably by ELISA/western blot. How will antibody pairs from Novus Biologics help me do that? Is a colorimetric assay possible with the antibody pair?

Name: Carboxypeptidase B1/CPB1 Antibody (438806)
Brand: Novus Biologicals
SKU: MAB2897
Price: 419 USD

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Any detection system can be employed when using the antibody pairs. The detection system will depend on the secondary used against the detection primary antibody from the pair, and the conjugate on the secondary antibody. The pair will work effectively in capturing the protein of interest, and subsequently detecting it with the second antibody in the pair for ELISA. This pair is not evaluated in Western Blot directly, but there Novus carries similar primary antibodies that we have that are validated in that application.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	WB, IP
Clone	438806
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated
Concentration	LYOPH

Catalog# & Formulation	Size	Price
Carrier Free MAB2897-SP

Immunogen	Mouse myeloma cell line NS0-derived recombinant human Carboxypeptidase B1/CPB1 His16-Tyr417 Accession # P15086
Specificity	Detects human Carboxypeptidase B1/CPB1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CPA1, rhCPA2, rhCPE, recombinant mouse (rm) CPA4, and rmCPB1 is observed.
Source	N/A
Isotype	IgG2a
Clonality	Monoclonal
Host	Mouse
Gene	CPB1
Purity Statement	Protein A or G purified from hybridoma culture supernatant
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.5 mg/mL in sterile PBS.

Carboxypeptidase B1/CPB1 Antibody (438806)

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Publications using MAB2897	Applications	Species
J Beumer, J Bauzá-Mart, TS Veth, V Geurts, C Boot, H Gilliam-Vi, SS Poulsen, FK Knop, W Wu, H Clevers Mapping prohormone processing by proteases in human enteroendocrine cells using genetically engineered organoid models Proceedings of the National Academy of Sciences of the United States of America, 2022-11-07;119(46):e2212057119. 2022-11-07 [PMID: 36343264] (IHC, Human)	IHC	Human
M Golizeh, K Winter, L Roussel, M Landekic, M Langelier, VG Loo, M Ndao, DC Vinh Fecal host biomarkers predicting severity of Clostridioides difficile infection JCI Insight, 2021-01-11;0(0):. 2021-01-11 [PMID: 33232301] (Western Blot, Human)	Western Blot	Human

Gene Symbol	CPB1
Uniprot	P15086()