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CapG Antibody (1F1)

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Western Blot: CapG Antibody (1F1) [H00000822-M01] - CAPG monoclonal antibody (M01), clone 1F1 Analysis of CAPG expression in HL-60.
Western Blot: CapG Antibody (1F1) [H00000822-M01] - CAPG monoclonal antibody (M01), clone 1F1 Analysis of CAPG expression in HL-60.
Immunocytochemistry/ Immunofluorescence: CapG Antibody (1F1) [H00000822-M01] - Analysis of monoclonal antibody to CAPG on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: CapG Antibody (1F1) [H00000822-M01] - Detection limit for recombinant GST tagged CAPG is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
1F1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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CapG Antibody (1F1) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
CAPG (AAH00728.1, 1 a.a. ~ 348 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MYTAIPQSGSPFPGSVQDPGLHVWRVEKLKPVPVAQENQGVFFSGDSYLVLHNGPEEVSHLHLWIGQQSSRDEQGACAVLAVHLNTLLGERPVQHREVQGNESDLFMSYFPRGLKYQEGGVESAFHKTSTGAPAAIKKLYQVKGKKNIRATERALNWDSFNTGDCFILDLGQNIFAWCGGKSNILERNKARDLALAIRDSERQGKAQVEIVTDGEEPAEMIQVLGPKPALKEGNPEEDLTADKANAQAAALYKVSDATGQMNLTKVADSSPFALELLISDDCFVLDNGLCGKIYIWKGRKANEKERQAALQVAEGFISRMQYAPNTQVEILPQGRESPIFKQFFKDWK
Specificity
CAPG - capping protein (actin filament), gelsolin-like
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CAPG
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CapG Antibody (1F1)

  • actin-regulatory protein CAP-G
  • AFCP
  • CapG
  • capping protein (actin filament), gelsolin-like
  • gelsolin-like capping protein
  • macrophage capping protein
  • macrophage-capping protein
  • MCPActin regulatory protein CAP-G

Background

This gene encodes a member of the gelsolin/villin family of actin-regulatory proteins. The encoded protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, the encoded protein contributes to the control of actin-based motility in non-muscle cells. Alternatively spliced transcript variants have been observed, but have not been fully described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CAPG
Entrez
OMIM
Uniprot