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Calpain 2 Antibody (OTI3G1) - Azide and BSA Free

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Western Blot: Calpain 2 Antibody (OTI3G1) - Azide and BSA Free [NBP2-70334] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Calpain 2.
Western Blot: Calpain 2 Antibody (OTI3G1) - Azide and BSA Free [NBP2-70334] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Calpain 2.
Immunohistochemistry: Calpain 2 Antibody (OTI3G1) - Azide and BSA Free [NBP2-70334] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Calpain 2 Antibody (OTI3G1) - Azide and BSA Free [NBP2-70334] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Calpain 2 Antibody (OTI3G1) - Azide and BSA Free [NBP2-70334] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Calpain 2 Antibody (OTI3G1) - Azide and BSA Free [NBP2-70334] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, IHC
Clone
OTI3G1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-70334AF350
Alexa Fluor 405
NBP2-70334AF405
Alexa Fluor 488
NBP2-70334AF488
Alexa Fluor 532
NBP2-70334AF532
Alexa Fluor 594
NBP2-70334AF594
Alexa Fluor 647
NBP2-70334AF647
Alexa Fluor 700
NBP2-70334AF700
Alexa Fluor 750
NBP2-70334AF750
Allophycocyanin
NBP2-70334APC
Biotin
NBP2-70334B
CoraFluor 1
NBP2-70334CL1
DyLight 350
NBP2-70334UV
DyLight 405
NBP2-70334V
DyLight 488
NBP2-70334G
DyLight 550
NBP2-70334R
DyLight 594
NBP2-70334DL594
DyLight 650
NBP2-70334C
DyLight 680
NBP2-70334FR
DyLight 755
NBP2-70334IR
FITC
NBP2-70334F
HRP
NBP2-70334H
Janelia Fluor 525
NBP2-70334JF525
Janelia Fluor 549
NBP2-70334JF549
Janelia Fluor 585
NBP2-70334JF585
Janelia Fluor 635
NBP2-70334JF635
Janelia Fluor 646
NBP2-70334JF646
Janelia Fluor 669
NBP2-70334JF669
PE
NBP2-70334PE
PerCP
NBP2-70334PCP
Unconjugated
NBP2-46063
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-46063
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Calpain 2 Antibody (OTI3G1) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human CAPN2(NP_001739) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CAPN2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
79.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Calpain 2 Antibody (OTI3G1) - Azide and BSA Free

  • Calcium-activated neutral proteinase 2
  • calpain 2, (m/II) large subunit
  • calpain 2, large [catalytic] subunit
  • calpain 2, large subunit
  • Calpain large polypeptide L2
  • Calpain M-type
  • calpain, large polypeptide L2
  • calpain-2 catalytic subunit
  • Calpain-2 large subunit
  • CANP 2
  • CANPL2CANP2
  • CANPml
  • EC 3.4.22
  • EC 3.4.22.53
  • FLJ39928
  • M-calpain
  • mCANP
  • millimolar-calpain

Background

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammaliancalpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist ofheterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This geneencodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites inthe 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene.(provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Calpain 2 Antibody
(OTI3G1) - Azide and ...
NBP2-70334
Calpain 2 Antibody (OTI3G1) - Azide and BSA Free
Species: Hu, Mu, Rt, Ca
Applications: WB, IHC

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Bioinformatics

Gene Symbol CAPN2