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c-Maf Antibody (JE39-57)

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Western Blot: c-Maf Antibody (JE39-57) [NBP3-32197] - Western blot analysis of c-Maf on different lysates with Rabbit anti-c-Maf antibody (NBP3-32197) at 1/1,000 dilution. Lane 1: A549 cell lysate (10 ug/Lane) Lane 2: Raji cell lysate (10 ug/Lane) Lane 3: Mouse lung tissue lysate (20 ug/Lane) Lane 4: Mouse kidney tissue lysate (20 ug/Lane) Lane 5: Rat spleen tissue lysate (20 ug/Lane) Lane 6: Rat kidney tissue lysate (20 ug/Lane) Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32197) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
Western Blot: c-Maf Antibody (JE39-57) [NBP3-32197] - Western blot analysis of c-Maf on different lysates with Rabbit anti-c-Maf antibody (NBP3-32197) at 1/1,000 dilution. Lane 1: A549 cell lysate (10 ug/Lane) Lane 2: ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clone
JE39-57
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

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c-Maf Antibody (JE39-57) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Synthetic peptide within Human c-Maf aa 324-373 / 373. (Uniprot: O75444)
Localization
Nucleus.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
MAF
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for c-Maf Antibody (JE39-57)

  • Avian musculoaponeurotic fibrosarcoma (MAF) protooncogene
  • CCA4
  • c-maf proto-oncogene
  • cMaf
  • c-Maf
  • MAF2
  • MGC71685
  • Proto-oncogene c-Maf
  • T lymphocyte c-maf long form
  • transcription factor Maf
  • v-maf musculoaponeurotic fibrosarcoma (avian) oncogene homolog
  • v-maf musculoaponeurotic fibrosarcoma oncogene homolog (avian)
  • V-maf musculoaponeurotic fibrosarcoma oncogene homolog

Background

c-Maf is part of the Maf family of transcription factors that possess a conserved basic region and a leucine zipper domain that mediate DNA and protein-protein binding and plays an important role in tissue-specific gene regulation and cell differentiation. c-Maf is a transcriptional activator of interleukin 4 and 10 in T-cells, and is involved in lens fiber cell differentiation. c-Maf translocation has been observed in human multiple myeloma and may be oncogenic.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MAF