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c-jun Antibody (OTI3G2)

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Western Blot: Jun Antibody (3G2) [NBP2-02258] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Jun (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Jun.
Western Blot: Jun Antibody (3G2) [NBP2-02258] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Jun (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug ...read more
Immunocytochemistry/ Immunofluorescence: Jun Antibody (3G2) [NBP2-02258] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Jun.
Flow Cytometry: Jun Antibody (3G2) [NBP2-02258] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Jun antibody, and then analyzed by ...read more
Flow Cytometry: Jun Antibody (3G2) [NBP2-02258] - Analysis of Hela cells, using anti-Jun antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: Jun Antibody (3G2) [NBP2-02258] - Analysis of Jurkat cells, using anti-Jun antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
OTI3G2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.9 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71059AF350
Alexa Fluor 405
NBP2-71059AF405
Alexa Fluor 488
NBP2-71059AF488
Alexa Fluor 532
NBP2-71059AF532
Alexa Fluor 594
NBP2-71059AF594
Alexa Fluor 647
NBP2-71059AF647
Alexa Fluor 700
NBP2-71059AF700
Alexa Fluor 750
NBP2-71059AF750
Allophycocyanin
NBP2-71059APC
Biotin
NBP2-71059B
CoraFluor 1
NBP2-71059CL1
DyLight 350
NBP2-71059UV
DyLight 405
NBP2-71059V
DyLight 488
NBP2-71059G
DyLight 550
NBP2-71059R
DyLight 594
NBP2-71059DL594
DyLight 650
NBP2-71059C
DyLight 680
NBP2-71059FR
DyLight 755
NBP2-71059IR
FITC
NBP2-71059F
HRP
NBP2-71059H
Janelia Fluor 525
NBP2-71059JF525
Janelia Fluor 549
NBP2-71059JF549
Janelia Fluor 646
NBP2-71059JF646
Janelia Fluor 669
NBP2-71059JF669
PE
NBP2-71059PE
PerCP
NBP2-71059PCP
mFluor Violet 450 SE
NBP2-71059MFV450
mFluor Violet 500 SE
NBP2-71059MFV500
mFluor Violet 610 SE
NBP2-71059MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-71059
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c-jun Antibody (OTI3G2) Summary

Immunogen
Full length human recombinant protein of human JUN (NP_002219) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
JUN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:2000
Theoretical MW
35.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.9 mg/ml
Purity
Immunogen affinity purified

Alternate Names for c-jun Antibody (OTI3G2)

  • Activator protein 1
  • AP1
  • AP-1
  • cJun
  • c-Jun
  • enhancer-binding protein AP1
  • Jun activation domain binding protein
  • jun oncogene
  • jun proto-oncogene
  • JUN
  • Proto-oncogene c-Jun
  • transcription factor AP-1
  • V-jun avian sarcoma virus 17 oncogene homologp39
  • v-jun sarcoma virus 17 oncogene homolog (avian)
  • v-jun sarcoma virus 17 oncogene homolog

Background

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol JUN
Entrez