BubR1 Antibody - N-terminal Summary
Immunogen |
Recombinant protein corresponding to amino acids 10-190 of the mouse BubR1 protein. |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Sheep |
Gene |
BUB1B |
Purity |
Ammonium sulfate precipitation |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunohistochemistry
- Immunoprecipitation : 1-10 ug/ml
- Western Blot : 1-10 ug/ml
|
Application Notes |
Antibody detects Bub1b in mitotic cells seperated from NIH/3T3 cells by IP/Westen blot analysis. Detects a 130 kDa band. |
Packaging, Storage & Formulations
Storage |
Store at -20C. Avoid freeze-thaw cycles. |
Buffer |
Phosphate buffered saline |
Preservative |
0.08% Sodium Azide |
Purity |
Ammonium sulfate precipitation |
Alternate Names for BubR1 Antibody - N-terminal
Background
Mitotic checkpoint serine/threonine-protein kinase BUB1 beta (BUB1B), also known as BubR1, is a kinase involved in spindle checkpoint function. BUBR1 is essential for normal mitotic progression as it prevents cells from entering into anaphase too early. BUBR1 has been detected in human cancers. BubR-1 localizes to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation.
BubR1 antibodies are useful tools for cancer research and cell division studies.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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