![Western Blot: BPTF/FALZ Antibody [NB100-41418] - Reactivity of BPTF/FALZ antibodies with lysates from human cancer cells. Cell line-specific patterns were observed, with bands of the same mobility being detected across lines and detected with independent antibodies. The findings suggest the occurrence of multiple BPTF/FALZ-related protein species in human cancer cells. A representative western blot is shown. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31498079/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BPTF-FALZ-Antibody-Western-Blot-NB100-41418-img0015.jpg "Western Blot: BPTF/FALZ Antibody [NB100-41418] - Reactivity of BPTF/FALZ antibodies with lysates from human cancer cells. Cell line-specific patterns were observed, with bands of the same mobility being detected across lines and detected with independent antibodies. The findings suggest the occurrence of multiple BPTF/FALZ-related protein species in human cancer cells. A representative western blot is shown. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31498079/) licensed under a CC-BY license.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 1350 and 1400 of human Fetal Alzheimer Antigen (Bromodomain and PHD Domain Transcription Factor) using the numbering given in entry NP_872579.2 (GeneID 2186). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | BPTF |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections. |
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| Publications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | TBS and 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry-Paraffin: BPTF/FALZ Antibody [NB100-41418] - Section of human lung carcinoma. Antibody: Affinity purified rabbit antiFALZ/BPTF used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/BPTF-FALZ-Antibody-Immunohistochemistry-Paraffin-NB100-41418-img0014.jpg "Immunohistochemistry-Paraffin: BPTF/FALZ Antibody [NB100-41418] - Section of human lung carcinoma. Antibody: Affinity purified rabbit antiFALZ/BPTF used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB")
![Western Blot: BPTF/FALZ Antibody [NB100-41418] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-41418 used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).](http://images.novusbio.com/fullsize/BPTF-FALZ-Antibody-Western-Blot-NB100-41418-img0006.jpg "Western Blot: BPTF/FALZ Antibody [NB100-41418] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-41418 used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).")
![Western Blot: BPTF/FALZ Antibody [NB100-41418] - Detection of Human FALZ/BPTF by Western Blot. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FALZ/BPTF antibody NB100-41418 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/BPTF-FALZ-Antibody-Western-Blot-NB100-41418-img0012.jpg "Western Blot: BPTF/FALZ Antibody [NB100-41418] - Detection of Human FALZ/BPTF by Western Blot. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FALZ/BPTF antibody NB100-41418 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunoprecipitation: BPTF/FALZ Antibody [NB100-41418] - Detection of human FALZ/BPTF by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FALZ/BPTF antibody NB100-41418 used for IP at 6 ug per reaction. For blotting immunoprecipitated FALZ/BPTF, NB100-41418 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/BPTF-FALZ-Antibody-Immunoprecipitation-NB100-41418-img0013.jpg "Immunoprecipitation: BPTF/FALZ Antibody [NB100-41418] - Detection of human FALZ/BPTF by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FALZ/BPTF antibody NB100-41418 used for IP at 6 ug per reaction. For blotting immunoprecipitated FALZ/BPTF, NB100-41418 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: BPTF/FALZ Antibody [NB100-41418] - Identification of alternative BPTF species in human cancer cells.(A) Reactivity of anti-BPTF antibodies with lysates from human cancer cells. Cell line-specific patterns were observed, with bands of the same mobility being detected across lines & detected with independent antibodies. The findings suggest the occurrence of multiple BPTF-related protein species in human cancer cells. A representative western blot is shown. (B) Gel bands selected for BPTF Mass Spectrometry identification according to the localization of the BPTF signal detected by western blotting in MCF-7 cells. (Left, NP-40 lysis buffer, gel bands A1 & A2; Right, Laemmli buffer, B1 & B2). The detection of low molecular weight species upon direct cell lysis in Laemmli buffer strongly supports the notion that the findings do not result from artifactual proteolysis. (C) Sequence coverage of BPTF protein. Peptides identified by LC-MS/MS are highlighted in color. (D) BPTF peptide intensity (arbitrary units) calculated by MaxQuant in the gel bands A1, A2/B1, B2. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31498079), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-41418_rabbit-polyclonal-bptf-falz-antibody-310202415541985.jpg "Western Blot: BPTF/FALZ Antibody [NB100-41418] - Identification of alternative BPTF species in human cancer cells.(A) Reactivity of anti-BPTF antibodies with lysates from human cancer cells. Cell line-specific patterns were observed, with bands of the same mobility being detected across lines & detected with independent antibodies. The findings suggest the occurrence of multiple BPTF-related protein species in human cancer cells. A representative western blot is shown. (B) Gel bands selected for BPTF Mass Spectrometry identification according to the localization of the BPTF signal detected by western blotting in MCF-7 cells. (Left, NP-40 lysis buffer, gel bands A1 & A2; Right, Laemmli buffer, B1 & B2). The detection of low molecular weight species upon direct cell lysis in Laemmli buffer strongly supports the notion that the findings do not result from artifactual proteolysis. (C) Sequence coverage of BPTF protein. Peptides identified by LC-MS/MS are highlighted in color. (D) BPTF peptide intensity (arbitrary units) calculated by MaxQuant in the gel bands A1, A2/B1, B2. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31498079), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: BPTF/FALZ Antibody [NB100-41418] - Identification of alternative BPTF species in human cancer cells.(A) Reactivity of anti-BPTF antibodies with lysates from human cancer cells. Cell line-specific patterns were observed, with bands of the same mobility being detected across lines & detected with independent antibodies. The findings suggest the occurrence of multiple BPTF-related protein species in human cancer cells. A representative western blot is shown. (B) Gel bands selected for BPTF Mass Spectrometry identification according to the localization of the BPTF signal detected by western blotting in MCF-7 cells. (Left, NP-40 lysis buffer, gel bands A1 & A2; Right, Laemmli buffer, B1 & B2). The detection of low molecular weight species upon direct cell lysis in Laemmli buffer strongly supports the notion that the findings do not result from artifactual proteolysis. (C) Sequence coverage of BPTF protein. Peptides identified by LC-MS/MS are highlighted in color. (D) BPTF peptide intensity (arbitrary units) calculated by MaxQuant in the gel bands A1, A2/B1, B2. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31498079), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-41418_rabbit-polyclonal-bptf-falz-antibody-310202415533846.jpg "Western Blot: BPTF/FALZ Antibody [NB100-41418] - Identification of alternative BPTF species in human cancer cells.(A) Reactivity of anti-BPTF antibodies with lysates from human cancer cells. Cell line-specific patterns were observed, with bands of the same mobility being detected across lines & detected with independent antibodies. The findings suggest the occurrence of multiple BPTF-related protein species in human cancer cells. A representative western blot is shown. (B) Gel bands selected for BPTF Mass Spectrometry identification according to the localization of the BPTF signal detected by western blotting in MCF-7 cells. (Left, NP-40 lysis buffer, gel bands A1 & A2; Right, Laemmli buffer, B1 & B2). The detection of low molecular weight species upon direct cell lysis in Laemmli buffer strongly supports the notion that the findings do not result from artifactual proteolysis. (C) Sequence coverage of BPTF protein. Peptides identified by LC-MS/MS are highlighted in color. (D) BPTF peptide intensity (arbitrary units) calculated by MaxQuant in the gel bands A1, A2/B1, B2. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31498079), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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