![Western Blot: ING3 Antibody [NBP1-78100] - Western blot using purified ING3 antibody: detection of a band at ~55 kDa corresponding to ING3 in RKO cells transfected with ING3 (lane 2). Control RKO cells don't show detection of this specific band (lane 1). Identity of the non-specific bands at 33 kDa & 20 kDa has not been determined. Each lane contains approximately 10 ug of RKO whole cell lysate (ATCC# CRL-2577 - human colon cancer) separated on a 4-20% Tris-Glycine gel by SDS-PAGE & transferred to nitrocellulose. After blocking with 5% NF dry milk, membrane was probed with primary antibody 1:1,000. Overnight incubation at 4C followed by washes & reaction with a 1:20,000 IRDye(TM)800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at room temperature. IRDye(TM)800 fluorescence image captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://images.novusbio.com/fullsize/ING3-Antibody-Western-Blot-NBP1-78100-img0004.jpg "Western Blot: ING3 Antibody [NBP1-78100] - Western blot using purified ING3 antibody: detection of a band at ~55 kDa corresponding to ING3 in RKO cells transfected with ING3 (lane 2). Control RKO cells don't show detection of this specific band (lane 1). Identity of the non-specific bands at 33 kDa & 20 kDa has not been determined. Each lane contains approximately 10 ug of RKO whole cell lysate (ATCC# CRL-2577 - human colon cancer) separated on a 4-20% Tris-Glycine gel by SDS-PAGE & transferred to nitrocellulose. After blocking with 5% NF dry milk, membrane was probed with primary antibody 1:1,000. Overnight incubation at 4C followed by washes & reaction with a 1:20,000 IRDye(TM)800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at room temperature. IRDye(TM)800 fluorescence image captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Description | This is an affinity-purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | This affinity purified ING3 Antibody was prepared from whole Goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 285-310 of Human ING3 protein (Inhibitor of growth family, member 3). (Uniprot: Q9NXR8) |
| Specificity | Reactivity occurs against human p47 ING3 protein. This sequence only shows homology to isoform 1 for ING3. The sequence is not present in isoforms 2 and 3. However, 100% homology is on record for this protein from human, dog, rat, chimpanzee and orangutan. Cross reactivity with p47 ING3 protein from mouse and chicken sources is also expected based on high homology (one amino acid change in sequence) by BLAST. Reactivity with p47 ING3 proteins from other sources is not known. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | ING3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | This affinity purified antibody has been tested for use in ELISA against the immunizing peptide. Specific conditions for western blotting reactivity should be optimized by the end user. Expect a band at approximately 47 kDa in size corresponding to ING3 isoform 1 by western blotting in the appropriate cell lysate or extract. |
| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Purity | Immunogen affinity purified |
![Simple Western alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]](https://images.novusbio.com/images2/16253.jpg)
![Knockout Validated alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]](https://images.novusbio.com/images2/alphaFetoprotein_MAB1368_Knockout_Validated_21503.jpg)
![Knockout Validated alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]](https://images.novusbio.com/images2/alphaFetoprotein_MAB1368_Knockout_Validated_22402.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for ING3 Antibody (NBP1-78100)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![N/A: ING3 Antibody [NBP1-78100]](http://images.novusbio.com/fullsize/ING3-Antibody-N-A-NBP1-78100-img0003.jpg "N/A: ING3 Antibody [NBP1-78100]")
