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BNIP1 Antibody (OTI2B3)

Images

 
Western Blot: BNIP1 Antibody (2B3) [NBP2-46580] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BNIP1.
Immunohistochemistry: BNIP1 Antibody (2B3) [NBP2-46580] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: BNIP1 Antibody (2B3) [NBP2-46580] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: BNIP1 Antibody (2B3) [NBP2-46580] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: BNIP1 Antibody (2B3) [NBP2-46580] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: BNIP1 Antibody (2B3) [NBP2-46580] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: BNIP1 Antibody (2B3) [NBP2-46580] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI2B3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

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BNIP1 Antibody (OTI2B3) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 1-199 of human BNIP1 (NP_001196) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BNIP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Western Blot 1:2000
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BNIP1 Antibody (OTI2B3)

  • BCL2/adenovirus E1B 19 kDa protein-interacting protein 1
  • BCL2/adenovirus E1B 19kDa interacting protein 1
  • BCL2/adenovirus E1B 19kD-interacting protein 1
  • Nip1
  • SEC20
  • SEC20L
  • Transformation-related gene 8 protein
  • TRG-8
  • vesicle transport protein SEC20

Background

BNIP1 is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. Alternative splicing of this gene results in four protein products with identical N- and C-termini.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BNIP1