![Western Blot: EIF3C Antibody [NB100-511] - eIF3C expression in naive and activated (overnight) murine B cells. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/EIF3C-Antibody-Western-Blot-NB100-511-img0005.jpg "Western Blot: EIF3C Antibody [NB100-511] - eIF3C expression in naive and activated (overnight) murine B cells. Image submitted by a verified customer review.")
| Reactivity | Hu, Mu, Rt, Bv, Pm-OrSpecies Glossary |
| Applications | WB, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | A synthetic peptide derived from a sequence between residues 850 and 918 (the C-terminus) of human eukaryotic translation initiation factor 3, subunit 8, 110kDa using the numbering given in Swiss-Prot entry Q99613 (GeneID 8663). |
| Predicted Species | Rat (100%), Orangutan (100%), Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | EIF3C |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | EIF3C antibody validated for WB from a verified customer review. |
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| Theoretical MW | 105 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Hyun Yong Jin |
WB | Mouse | 02/28/2017 |
Summary
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot: EIF3C Antibody [NB100-511] - Detection of Human eIF3C/eIF3S8 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eIF3C/eIF3S8 antibody NB100-511 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/EIF3C-Antibody-Western-Blot-NB100-511-img0004.jpg "Western Blot: EIF3C Antibody [NB100-511] - Detection of Human eIF3C/eIF3S8 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eIF3C/eIF3S8 antibody NB100-511 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunoprecipitation: EIF3C Antibody [NB100-511] - Detection of human eIF3C/eIF3S8 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eIF3C/eIF3S8 antibody NB100-511 used for IP at 6 ug per reaction. eIF3C/eIF3S8 was inefficiently immunoprecipitated by rabbit anti-eIF3C/eIF3S8 antibody from Company B. For blotting immunoprecipitated eIF3C/eIF3S8, NB100-511 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/EIF3C-Antibody-Immunoprecipitation-NB100-511-img0006.jpg "Immunoprecipitation: EIF3C Antibody [NB100-511] - Detection of human eIF3C/eIF3S8 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eIF3C/eIF3S8 antibody NB100-511 used for IP at 6 ug per reaction. eIF3C/eIF3S8 was inefficiently immunoprecipitated by rabbit anti-eIF3C/eIF3S8 antibody from Company B. For blotting immunoprecipitated eIF3C/eIF3S8, NB100-511 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Bioactivity Sonic Hedgehog/Shh [Unconjugated]](https://images.novusbio.com/images2/Sonic_Hedgehog_464SH_2840.jpg)
![SDS-PAGE Sonic Hedgehog/Shh [Unconjugated]](https://images.novusbio.com/images2/Sonic_Hedgehog_464-SH_341.jpg)
![SEC-MALS Sonic Hedgehog/Shh [Unconjugated]](https://images.novusbio.com/images/464-sh_recombinant-mouse-sonic-hedgehog-shh-c25ii-n-terminus-sec-mals-642023224324.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
