![Western Blot: Eukaryotic translation initiation factor 1 Antibody (2B9) [NBP2-00702] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Eukaryotic translation initiation factor 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Eukaryotic translation initiation factor 1.](http://images.novusbio.com/fullsize/Eukaryotic-translation-initiation-factor-1-Antibody-2B9-Western-Blot-NBP2-00702-img0004.jpg "Western Blot: Eukaryotic translation initiation factor 1 Antibody (2B9) [NBP2-00702] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Eukaryotic translation initiation factor 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Eukaryotic translation initiation factor 1.")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | OTI2B9 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1.32 mg/ml |
| Immunogen | Full length human recombinant protein of human EIF1 (NP_005792) produced in HEK293T cell. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | EIF1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 12.6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1.32 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: Eukaryotic translation initiation factor 1 Antibody (2B9) [NBP2-00702] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Eukaryotic translation initiation factor 1.](http://images.novusbio.com/fullsize/Eukaryotic-translation-initiation-factor-1-Antibody-2B9-Immunocytochemistry-Immunofluorescence-NBP2-00702-img0007.jpg "Immunocytochemistry/Immunofluorescence: Eukaryotic translation initiation factor 1 Antibody (2B9) [NBP2-00702] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Eukaryotic translation initiation factor 1.")
![Immunohistochemistry-Paraffin: Eukaryotic translation initiation factor 1 Antibody (2B9) [NBP2-00702] - Staining of paraffin-embedded Human lymph node tissue using anti-Eukaryotic translation initiation factor 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Eukaryotic-translation-initiation-factor-1-Antibody-2B9-Immunohistochemistry-Paraffin-NBP2-00702-img0006.jpg "Immunohistochemistry-Paraffin: Eukaryotic translation initiation factor 1 Antibody (2B9) [NBP2-00702] - Staining of paraffin-embedded Human lymph node tissue using anti-Eukaryotic translation initiation factor 1 mouse monoclonal antibody.")
![Flow Cytometry: Eukaryotic translation initiation factor 1 Antibody (2B9) [NBP2-00702] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Eukaryotic translation initiation factor 1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Eukaryotic-translation-initiation-factor-1-Antibody-2B9-Flow-Cytometry-NBP2-00702-img0003.jpg "Flow Cytometry: Eukaryotic translation initiation factor 1 Antibody (2B9) [NBP2-00702] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Eukaryotic translation initiation factor 1 antibody, and then analyzed by flow cytometry.")
![Immunocytochemistry/Immunofluorescence: Eukaryotic translation initiation factor 1 Antibody (2B9) [NBP2-00702] - Immunofluorescent staining of HeLa cells using anti-Eukaryotic translation initiation factor 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Eukaryotic-translation-initiation-factor-1-Antibody-2B9-Immunocytochemistry-Immunofluorescence-NBP2-00702-img0005.jpg "Immunocytochemistry/Immunofluorescence: Eukaryotic translation initiation factor 1 Antibody (2B9) [NBP2-00702] - Immunofluorescent staining of HeLa cells using anti-Eukaryotic translation initiation factor 1 mouse monoclonal antibody.")
![Flow Cytometry: Eukaryotic translation initiation factor 1 Antibody (2B9) [NBP2-00702] - Analysis of Hela cells, using anti-Eukaryotic translation initiation factor 1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Eukaryotic-translation-initiation-factor-1-Antibody-2B9-Flow-Cytometry-NBP2-00702-img0001.jpg "Flow Cytometry: Eukaryotic translation initiation factor 1 Antibody (2B9) [NBP2-00702] - Analysis of Hela cells, using anti-Eukaryotic translation initiation factor 1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Flow Cytometry: Eukaryotic translation initiation factor 1 Antibody (2B9) [NBP2-00702] - Analysis of Jurkat cells, using anti-Eukaryotic translation initiation factor 1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Eukaryotic-translation-initiation-factor-1-Antibody-2B9-Flow-Cytometry-NBP2-00702-img0002.jpg "Flow Cytometry: Eukaryotic translation initiation factor 1 Antibody (2B9) [NBP2-00702] - Analysis of Jurkat cells, using anti-Eukaryotic translation initiation factor 1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
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