Novus Biologicals products are now on bio-techne.com

BLIMP1/PRDM1 Antibody (646702) [Unconjugated]

Images

 
Western blot shows lysates of U266 human myeloma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human BLIMP1/PRDM1 Monoclonal Antibody (Catalog # MAB36081) followed by HRP-conjugated Anti-Mouse IgG ...read more
Simple Western lane view shows lysates of U266 human myeloma cell line, loaded at 0.2 mg/mL. A specific band was detected for BLIMP1/PRDM1 at approximately 114 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human ...read more
U266 human myeloma cell line was stained with Mouse Anti-Human BLIMP1/PRDM1 Monoclonal Antibody (Catalog # MAB36081, filled histogram) or isotype control antibody (MAB002, open histogram) followed by anti-Mouse IgG ...read more
MM.1S cells were stained with Mouse Anti-Human BLIMP1/PRDM1 Monoclonal Antibody (Catalog # MAB36081, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, Flow
Clone
646702
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

BLIMP1/PRDM1 Antibody (646702) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human BLIMP1/PRDM1
Lys667-Cys789
Accession # O75626
Specificity
Detects human BLIMP1/PRDM1 in Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PRDM1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
MAB36081 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BLIMP1/PRDM1 Antibody (646702) [Unconjugated]

  • Beta-interferon gene positive regulatory domain I-binding factor
  • beta-interferon gene positive-regulatory domain I binding factor
  • BLIMP1
  • BLIMP-1
  • BLIMP1MGC118925
  • blmp-1
  • B-lymphocyte-induced maturation protein 1
  • MGC118922
  • MGC118923
  • Positive regulatory domain I-binding factor 1
  • PR domain containing 1, with ZNF domain
  • PR domain zinc finger protein 1
  • PR domain-containing protein 1
  • PRDI-BF1MGC118924
  • PRDI-binding factor 1
  • PRDI-binding factor-1
  • PRDM1
  • PR-domain zinc finger protein 1
  • ZNFPR1A1

Background

Human BLIMP1 (B lymphocyte-induced maturation protein 1, also known as PRDM1) is a 91 kDa zinc-finger transcriptional repressor that promotes B cell maturation into plasma cells. It is 789 amino acids (aa) in length, and contains an N-terminal S-E-T domain (aa 64-170) and four C-terminal C2H2-type zinc-finger motifs (aa 539‑645). The SET domain interacts with chromatin modifers, while the zinc fingers bind to DNA. There is one 80 kDa, 691 aa alternate splice form that utilizes an intenal start site. This results in a substitution of three aa for the first 101 aa of the long form, and the loss of the SET domain. At least 10 mutations exist, resulting in proteins of 61‑603 aa in length. Over aa 667-789, human BLIMP1 is 89% aa identical to mouse BLIMP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-59786
Species: Hu
Applications: ICC/IF, IHC, IHC-P
MAB3487
Species: Hu, Mu
Applications: Flow, ICC, IHC, mIF, Simple Western, WB
NBP1-77681
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, KD, WB
AF2780
Species: Hu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, WB
AF5525
Species: Hu
Applications: IHC, WB
6507-IL/CF
Species: Hu
Applications: BA
202-IL
Species: Hu
Applications: BA
NB600-302
Species: Bv, Dr, Hu, Mu
Applications: ChIP, ELISA, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, S-ELISA, Simple Western, WB
NBP1-89644
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-30955
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
AF594
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, Neut, WB
AF632
Species: Hu
Applications: AgAct, Simple Western, WB
7268-CT
Species: Hu
Applications: BA
NBP1-19371
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
DY417
Species: Mu
Applications: ELISA
NBP1-91011
Species: Hu
Applications: IHC, IHC-Fr, IHC-P, WB
AF114
Species: Mu
Applications: CyTOF-ready, Flow, ICC, IHC, WB
M6000B
Species: Mu
Applications: ELISA
M5000
Species: Mu
Applications: ELISA
MAB36081
Species: Hu
Applications: WB, Simple Western, Flow

Publications for BLIMP1/PRDM1 Antibody (MAB36081)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Flow Cytometry, ICC, Western Blot.


Filter By Application
Flow Cytometry
(1)
ICC
(1)
Western Blot
(3)
All Applications
Filter By Species
Human
(5)
All Species
Showing Publications 1 - 7 of 7.
Publications using MAB36081 Applications Species
X Dai, JJ Park, Y Du, Z Na, SZ Lam, RD Chow, PA Renauer, J Gu, S Xin, Z Chu, C Liao, P Clark, H Zhao, S Slavoff, S Chen Massively parallel knock-in engineering of human T cells Nature Biotechnology, 2023-01-26;0(0):. 2023-01-26 [PMID: 36702900] (Western Blot, Human) Western Blot Human
J Ahlers, A Mantei, L Lozza, M Stäber, F Heinrich, P Bacher, T Hohnstein, L Menzel, SG Yüz, D Alvarez-Si, AR Bickenbach, C Weidinger, N Mockel-Ten, AA Kühl, B Siegmund, J Maul, C Neumann, A Scheffold A Notch/STAT3-driven Blimp-1/c-Maf-dependent molecular switch induces IL-10 expression in human CD4+ T cells and is defective in Crohn�s disease patients Mucosal Immunology, 2022-02-15;0(0):. 2022-02-15 [PMID: 35169232] (Flow Cytometry, Human) Flow Cytometry Human
Yoji Kojima, Chika Yamashiro, Yusuke Murase, Yukihiro Yabuta, Ikuhiro Okamoto, Chizuru Iwatani et al. GATA transcription factors, SOX17 and TFAP2C, drive the human germ-cell specification program Life Science Alliance 5/1/2021 [PMID: 33608411]
Fang Fang, Zili Li, Qian Zhao, Zhen Ye, Xiuli Gu, Feng Pan et al. Induced Pluripotent Stem Cells Derived From Two Idiopathic Azoospermia Patients Display Compromised Differentiation Potential for Primordial Germ Cell Fate Frontiers in Cell and Developmental Biology 6/25/2020 [PMID: 32671061]
D Chen, W Liu, J Zimmerman, WA Pastor, R Kim, L Hosohama, J Ho, M Aslanyan, JJ Gell, SE Jacobsen, AT Clark The TFAP2C-Regulated OCT4 Naive Enhancer Is Involved in Human Germline Formation Cell Rep, 2018-12-26;25(13):3591-3602.e5. 2018-12-26 [PMID: 30590035] (ICC, Human) ICC Human
N Wada, JI Ikeda, H Tanaka, H Sakakita, M Hori, Y Ikehara, E Morii Effect of plasma-activated medium on the decrease of tumorigenic population in lymphoma Pathol. Res. Pract., 2017-04-20;0(0):. 2017-04-20 [PMID: 28554762] (Western Blot, Human) Western Blot Human
Li S, Pal R, Monaghan SA, Schafer P, Ouyang H, Mapara M, Galson DL, Lentzsch S IMiD immunomodulatory compounds block C/EBP{beta} translation through eIF4E down-regulation resulting in inhibition of MM. Blood, 2011-03-09;117(19):5157-65. 2011-03-09 [PMID: 21389327] (Western Blot, Human) Western Blot Human

Reviews for BLIMP1/PRDM1 Antibody (MAB36081) (0)

There are no reviews for BLIMP1/PRDM1 Antibody (MAB36081). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for BLIMP1/PRDM1 Antibody (MAB36081) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional BLIMP1/PRDM1 Products

Blogs on BLIMP1/PRDM1.

Tired T cells: Hypoxia Drives T cell Exhaustion in the Tumor Microenvironment
By Hunter MartinezThe paradigm shifting view of the immune system being leveraged to target cancer has led to numerous therapeutic breakthroughs. One major cell group responsible for this revelation is a T cell. ...  Read full blog post.

Read our latest blog and use the new citation tool on bio-techne.com

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our BLIMP1/PRDM1 Antibody (646702) [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol PRDM1
Uniprot