![Immunocytochemistry/Immunofluorescence: BIN1 Antibody (99F) [NBP2-21674] - Cells: C2C12 cells during growth or differentiation. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: BIN-1 (Exon 10 specific, 99F) monoclonal antibody. Secondary antibody: mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: BIN1 is nuclear and cytoplasmic. Staining: BIN 1 as green fluorescent signal.](http://images.novusbio.com/fullsize/BIN1-Antibody-99F-Immunocytochemistry-Immunofluorescence-NBP2-21674-img0002.jpg "Immunocytochemistry/Immunofluorescence: BIN1 Antibody (99F) [NBP2-21674] - Cells: C2C12 cells during growth or differentiation. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: BIN-1 (Exon 10 specific, 99F) monoclonal antibody. Secondary antibody: mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: BIN1 is nuclear and cytoplasmic. Staining: BIN 1 as green fluorescent signal.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, DB, ELISA, ICC/IF, IP |
| Clone | 99F |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | This antibody was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | BIN1 Antibody (99F) was produced in mouse by repeated immunizations with BIN1 polypeptide followed by hybridoma development. (Uniprot: O00499) |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | BIN1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This product has been tested for use in ELISA, Western Blot, IP, and IF. Specific conditions for reactivity should be optimized by the end user. |
| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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![Immunoprecipitation of Mouse anti-BIN1 antibody. Immunoprecipitates were separated by SDS-PAGE and visualized by fluorography. The differentiation-associated (Exon 10+) form of Bin1 is recognized by using Bin1 (Exon 10 specific, 99F) monoclonal antibody. Lane 1: BIN1 [35ug]. Primary antibody: Anti-BIN1 (Exon 10 specific, 99F) monoclonal antibody at 1:400 for overnight at 2-8C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted MW: 64.7 kDa. Observed MW: ~65 kDa for BIN-1.](http://images.novusbio.com/fullsize/nbp2-21674_mouse-monoclonal-bin1-antibody-99f-2352023180522.jpg "Immunoprecipitation of Mouse anti-BIN1 antibody. Immunoprecipitates were separated by SDS-PAGE and visualized by fluorography. The differentiation-associated (Exon 10+) form of Bin1 is recognized by using Bin1 (Exon 10 specific, 99F) monoclonal antibody. Lane 1: BIN1 [35ug]. Primary antibody: Anti-BIN1 (Exon 10 specific, 99F) monoclonal antibody at 1:400 for overnight at 2-8C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted MW: 64.7 kDa. Observed MW: ~65 kDa for BIN-1.")
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