ABCA7 Antibody (7A1-144) Summary
Immunogen |
Mouse ABCA7 stably-transfected HeLa cells. [UniProt# Q91V24] |
Localization |
Cell Membrane and Cytoplasmic |
Isotype |
IgG2a Kappa |
Clonality |
Monoclonal |
Host |
Rat |
Gene |
ABCA7 |
Purity |
Protein G purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Flow Cytometry 1:500 - 1:4000
- Immunohistochemistry
- Immunohistochemistry-Paraffin 1:100 - 1:250
- Western Blot 1:2000
|
Application Notes |
This ABCA7 antibody is useful for Flow Cytometry and Western Blot, where a band is observed at ~240 kDa. This ABCA7 antibody is not reactive in ELISA. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Theoretical MW |
240 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
Buffer |
PBS |
Preservative |
0.05% Sodium Azide |
Concentration |
1.0 mg/ml |
Purity |
Protein G purified |
Alternate Names for ABCA7 Antibody (7A1-144)
Background
ABCA7 (ATP-binding cassette sub-family A member 7) belongs to "A" subfamily of ABC transporters that predominantly transport lipids and other lipophilic molecules across membranes. ABCA7 shows higher expression in brain, lung, adrenal gland and spleen as well as hematopoietic tissues. It localizes to cell membrane ruffles/phagocytic cups of activated macrophages; to cytoplasm (Golgi and endosomes) of resting macrophages, and to the apical brush border of cells in the proximal tubules of kidney. It is also detected in both the plasma membrane and in intracellular compartments in myelo-lymphatic tissues (peripheral leukocytes, thymus, spleen, and bone marrow). ABCA7 facilitates the transport of phospholipids and, to a lesser extent, cholesterol, across cell membranes to extracellular apoAI and apoE discoidal lipoprotein complexes. Moreover, it implicates in macrophages mediated phagocytosis of apoptotic cells, and binds APOA1 for involving in apolipoprotein-mediated phospholipid efflux from cells. ABCA7 also mediate cholesterol efflux, and can regulate the cellular ceramide homeostasis during keratinocytes differentiation.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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