BIN1 Antibodies
We offer BIN1 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each BIN1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our BIN1 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the BIN1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our BIN1 polyclonal antibodies and browse our BIN1 monoclonal antibody catalog.
Alternate Names for BIN1 Antibodies
anti-BIN1 antibody, anti-AMPH2 antibody, anti-Amphiphysin II antibody, anti-Amphiphysin-like protein antibody, anti-AMPHLDKFZp547F068 antibody, anti-box dependant MYC interacting protein 1 antibody, anti-bridging integrator 1Box-dependent myc-interacting protein 1 antibody, anti-MGC10367 antibody, anti-myc box-dependent-interacting protein 1 antibody, anti-SH3P9 antibody
11 Results for "bin1" in Primary Antibodies
![Western Blot: BIN1 Antibody [NBP1-89102] - Analysis in human cell lines Caco-2 and MCF-7 using anti-BIN1 antibody. Corresponding BIN1 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.](http://images.novusbio.com/fullsize/BIN1-Antibody-Western-Blot-NBP1-89102-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: BIN1 Antibody [NBP1-89102] - Analysis in human cell lines Caco-2 and MCF-7 using anti-BIN1 antibody. Corresponding BIN1 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: BIN1 Antibody [NBP1-89102] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/BIN1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89102-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: BIN1 Antibody [NBP1-89102] - Staining in human skeletal muscle and heart muscle tissues using anti-BIN1 antibody. Corresponding BIN1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/BIN1-Antibody-Immunohistochemistry-Paraffin-NBP1-89102-img0012.jpg)
![Western Blot: BIN1 Antibody [NBP1-89102] - Analysis in control (vector only transfected HEK293T lysate) and BIN1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/BIN1-Antibody-Western-Blot-NBP1-89102-img0015.jpg)
![Immunohistochemistry-Paraffin: BIN1 Antibody [NBP1-89102] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/BIN1-Antibody-Immunohistochemistry-Paraffin-NBP1-89102-img0006.jpg)
![Immunohistochemistry-Paraffin: BIN1 Antibody [NBP1-89102] - Staining of human heart muscle shows low expression as expected.](http://images.novusbio.com/fullsize/BIN1-Antibody-Immunohistochemistry-Paraffin-NBP1-89102-img0011.jpg)
![Western Blot: BIN1 Antibody [NBP2-15587] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with BIN1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BIN1-Antibody-Western-Blot-NBP2-15587-img0009.jpg "Western Blot: BIN1 Antibody [NBP2-15587] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with BIN1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: BIN1 Antibody [NBP2-15587] - BIN1 antibody detects BIN1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BIN1 stained by BIN1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Scale bar= 10 um.](http://images.novusbio.com/fullsize/BIN1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15587-img0008.jpg)
![Immunohistochemistry-Paraffin: BIN1 Antibody [NBP2-15587] - BIN1 antibody detects BIN1 protein at cytoplasm in human papillary adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human papillary adenocarcinoma. BIN1 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/BIN1-Antibody-Immunohistochemistry-Paraffin-NBP2-15587-img0005.jpg)
![Western Blot: BIN1 Antibody [NBP2-15587] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BIN1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BIN1-Antibody-Western-Blot-NBP2-15587-img0006.jpg)
![Western Blot: BIN1 Antibody [NBP2-15587] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BIN1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BIN1-Antibody-Western-Blot-NBP2-15587-img0007.jpg)
![Immunohistochemistry-Paraffin: BIN1 Antibody [NBP2-15587] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/BIN1-Antibody-Immunohistochemistry-Paraffin-NBP2-15587-img0003.jpg)
![Western Blot: BIN1 Antibody (3B6F10) [NBP2-61714] - Analysis using BIN1 mAb against HEK293 (1) and BIN1 (AA: 189-398)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/BIN1-Antibody-3B6F10-Western-Blot-NBP2-61714-img0005.jpg "Western Blot: BIN1 Antibody (3B6F10) [NBP2-61714] - Analysis using BIN1 mAb against HEK293 (1) and BIN1 (AA: 189-398)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry: BIN1 Antibody (3B6F10) [NBP2-61714] - Analysis of paraffin-embedded cervical cancer tissues using BIN1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/BIN1-Antibody-3B6F10-Immunohistochemistry-NBP2-61714-img0003.jpg)
![Flow Cytometry: BIN1 Antibody (3B6F10) [NBP2-61714] - Analysis of Hela cells using BIN1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/BIN1-Antibody-3B6F10-Flow-Cytometry-NBP2-61714-img0002.jpg)
![Western Blot: BIN1 Antibody (3B6F10) [NBP2-61714] - Analysis using BIN1 mAb against human BIN1 (AA: 189-398) recombinant protein. (Expected MW is 47.1 kDa)](http://images.novusbio.com/fullsize/BIN1-Antibody-3B6F10-Western-Blot-NBP2-61714-img0004.jpg)
![Western Blot: BIN1 Antibody (3B6F10) [NBP2-61714] - Analysis using BIN1 mouse mAb against C2C12 (1), A431 (2), HEK293 (3), and MCF-7 (4) cell lysate.](http://images.novusbio.com/fullsize/BIN1-Antibody-3B6F10-Western-Blot-NBP2-61714-img0006.jpg)
![ELISA: BIN1 Antibody (3B6F10) [NBP2-61714] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/BIN1-Antibody-3B6F10-ELISA-NBP2-61714-img0001.jpg)
![Immunohistochemistry-Paraffin: BIN1 Antibody (2F11) [NBP2-21675] - Mouse Anti-BIN1 antibody. Tissue: skeletal muscle from BIN-1 wild type and null mouse. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. BIN-1 antibody for 1hr at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: BIN1 is nuclear and cytoplasmic. Staining: BIN1 as precipitated brown signal.](http://images.novusbio.com/fullsize/BIN1-Antibody-2F11-Immunohistochemistry-Paraffin-NBP2-21675-img0001.jpg "Immunohistochemistry-Paraffin: BIN1 Antibody (2F11) [NBP2-21675] - Mouse Anti-BIN1 antibody. Tissue: skeletal muscle from BIN-1 wild type and null mouse. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. BIN-1 antibody for 1hr at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: BIN1 is nuclear and cytoplasmic. Staining: BIN1 as precipitated brown signal.")
![Western Blot: BIN1 Antibody [NBP1-46170] - Detection of Human Bin1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-Bin1 antibody NBP1-46170 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/BIN1-Antibody-Western-Blot-NBP1-46170-img0007.jpg "Western Blot: BIN1 Antibody [NBP1-46170] - Detection of Human Bin1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-Bin1 antibody NBP1-46170 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: BIN1 Antibody [NBP1-46170] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Bin1 used at a dilution of 1:1,000 (1ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit .](http://images.novusbio.com/fullsize/BIN1-Antibody-Immunohistochemistry-Paraffin-NBP1-46170-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: BIN1 Antibody [NBP1-46170] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Bin1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Bin1 was also immunoprecipitated by rabbit anti-Bin1 antibody NBP1-46169, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/BIN1-Antibody-Western-Blot-NBP1-46170-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: BIN1 Antibody [NBP1-46170] - Detection of human Bin1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Bin1 antibody NBP1-46170 used for IP at 6 ug per reaction. Bin1 was also immunoprecipitated by another rabbit anti-Bin1 antibody. For blotting immunoprecipitated Bin1, NBP1-46170 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/BIN1-Antibody-Immunoprecipitation-NBP1-46170-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: BIN1 Antibody (99F) [NBP2-21674] - Cells: C2C12 cells during growth or differentiation. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: BIN-1 (Exon 10 specific, 99F) monoclonal antibody. Secondary antibody: mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: BIN1 is nuclear and cytoplasmic. Staining: BIN 1 as green fluorescent signal.](http://images.novusbio.com/fullsize/BIN1-Antibody-99F-Immunocytochemistry-Immunofluorescence-NBP2-21674-img0002.jpg "Immunocytochemistry/Immunofluorescence: BIN1 Antibody (99F) [NBP2-21674] - Cells: C2C12 cells during growth or differentiation. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: BIN-1 (Exon 10 specific, 99F) monoclonal antibody. Secondary antibody: mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: BIN1 is nuclear and cytoplasmic. Staining: BIN 1 as green fluorescent signal.")
![Immunoprecipitation of Mouse anti-BIN1 antibody. Immunoprecipitates were separated by SDS-PAGE and visualized by fluorography. The differentiation-associated (Exon 10+) form of Bin1 is recognized by using Bin1 (Exon 10 specific, 99F) monoclonal antibody. Lane 1: BIN1 [35ug]. Primary antibody: Anti-BIN1 (Exon 10 specific, 99F) monoclonal antibody at 1:400 for overnight at 2-8C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted MW: 64.7 kDa. Observed MW: ~65 kDa for BIN-1.](http://images.novusbio.com/fullsize/nbp2-21674_mouse-monoclonal-bin1-antibody-99f-2352023180522.jpg)
![Western Blot: BIN1 Antibody (99D) [NBP2-21673] - Lane 1: Keratinocyte derived from Bin1 wild type mice. Lane 2: Keratinocyte derived from Bin1 null mice. Load: 35 ug per lane. BIN1 monoclonal Antibody. Secondary antibody: Dye800 mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 1xPBS, 0.4% Tween-20. Other band: non-specific.](http://images.novusbio.com/fullsize/BIN1-Antibody-99D-Western-Blot-NBP2-21673-img0001.jpg "Western Blot: BIN1 Antibody (99D) [NBP2-21673] - Lane 1: Keratinocyte derived from Bin1 wild type mice. Lane 2: Keratinocyte derived from Bin1 null mice. Load: 35 ug per lane. BIN1 monoclonal Antibody. Secondary antibody: Dye800 mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 1xPBS, 0.4% Tween-20. Other band: non-specific.")
![Immunohistochemistry-Paraffin: BIN1 Antibody [NBP1-89103] - Staining in human skeletal muscle and skin tissues using anti-BIN1 antibody. Corresponding BIN1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/BIN1-Antibody-Immunohistochemistry-Paraffin-NBP1-89103-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: BIN1 Antibody [NBP1-89103] - Staining in human skeletal muscle and skin tissues using anti-BIN1 antibody. Corresponding BIN1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry: BIN1 Antibody [NBP1-89103] - Staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells and glial cells.](http://images.novusbio.com/fullsize/BIN1-Antibody-Immunohistochemistry-NBP1-89103-img0003.jpg)
![Immunohistochemistry-Paraffin: BIN1 Antibody [NBP1-89103] - Staining of human skeletal muscle shows high expression.](http://images.novusbio.com/fullsize/BIN1-Antibody-Immunohistochemistry-Paraffin-NBP1-89103-img0005.jpg)
![Immunohistochemistry-Paraffin: BIN1 Antibody [NBP1-89103] - Staining of human skin shows low expression as expected.](http://images.novusbio.com/fullsize/BIN1-Antibody-Immunohistochemistry-Paraffin-NBP1-89103-img0006.jpg)
![Western Blot: BIN1 Antibody (12A) [NBP2-21689] - Mouse Anti-BIN-1 Antibody. Lane 1: LNCap. Lane 2: WM1341D. Lane 3: WM164. Lane 4: WM983A. Lane 5: WM793. Lane 6: WM239A. Lane 7: WM35. Load: 35 ug per lane. BIN-1 antibody at 1:400 for overnight at 4C. Secondary antibody: Dye800 mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Blocking Buffer overnight at 4C.](http://images.novusbio.com/fullsize/BIN1-Antibody-12A-Western-Blot-NBP2-21689-img0001.jpg "Western Blot: BIN1 Antibody (12A) [NBP2-21689] - Mouse Anti-BIN-1 Antibody. Lane 1: LNCap. Lane 2: WM1341D. Lane 3: WM164. Lane 4: WM983A. Lane 5: WM793. Lane 6: WM239A. Lane 7: WM35. Load: 35 ug per lane. BIN-1 antibody at 1:400 for overnight at 4C. Secondary antibody: Dye800 mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Blocking Buffer overnight at 4C.")
![Western Blot: BIN1 Antibody (2C7) [H00000274-M03] - Analysis of BIN1 expression in transfected 293T cell line by BIN1 monoclonal antibody (M03), clone 2C7. Lane 1: BIN1 transfected lysatE (53 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/BIN1-Antibody-2C7-Western-Blot-H00000274-M03-img0003.jpg "Western Blot: BIN1 Antibody (2C7) [H00000274-M03] - Analysis of BIN1 expression in transfected 293T cell line by BIN1 monoclonal antibody (M03), clone 2C7. Lane 1: BIN1 transfected lysatE (53 KDa). Lane 2: Non-transfected lysate.")
![ELISA: BIN1 Antibody (2C7) [H00000274-M03] - Detection limit for recombinant GST tagged BIN1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/BIN1-Antibody-2C7-ELISA-H00000274-M03-img0002.jpg)
![Western Blot: BIN1 Antibody (1H1) [H00000274-M01] - Analysis of BIN1 expression in transfected 293T cell line by BIN1 monoclonal antibody (M01), clone 1H1.Lane 1: BIN1 transfected lysate(53 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/BIN1-Antibody-1H1-Western-Blot-H00000274-M01-img0003.jpg "Western Blot: BIN1 Antibody (1H1) [H00000274-M01] - Analysis of BIN1 expression in transfected 293T cell line by BIN1 monoclonal antibody (M01), clone 1H1.Lane 1: BIN1 transfected lysate(53 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: BIN1 Antibody (1H1) [H00000274-M01] - BIN1 monoclonal antibody (M01), clone 1H1 Analysis of BIN1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/BIN1-Antibody-1H1-Western-Blot-H00000274-M01-img0002.jpg)