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BHMT2 Antibody

Images

 
Western Blot: BHMT2 Antibody [NBP1-85826] - Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate
Orthogonal Strategies: Immunohistochemistry-Paraffin: BHMT2 Antibody [NBP1-85826] - Staining in human kidney and lymph node tissues using anti-BHMT2 antibody. Corresponding BHMT2 RNA-seq data are presented for ...read more
Western Blot: BHMT2 Antibody [NBP1-85826] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane ...read more
Immunohistochemistry-Paraffin: BHMT2 Antibody [NBP1-85826] - Staining of human kidney shows high expression.
Immunohistochemistry-Paraffin: BHMT2 Antibody [NBP1-85826] - Staining of human lymph node shows low expression as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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BHMT2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: FTFSASEDNMESKWEDVNAAACDLAREVAGKGDALVAGGICQTSIYKYQKDEA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BHMT2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
BHMT2 Protein (NBP1-85826PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for BHMT2 Antibody

  • betaine-homocysteine methyltransferase 2
  • betaine--homocysteine S-methyltransferase 2
  • EC 2.1.1.5
  • FLJ20001

Background

Homocysteine is a sulfur-containing amino acid that plays a crucial role in methylation reactions. Transfer of the methyl group from betaine to homocysteine creates methionine, which donates the methyl group to methylate DNA, proteins, lipids, and other intracellular metabolites. The protein encoded by this gene is one of two methyl transferases that can catalyze the transfer of the methyl group from betaine to homocysteine. Anomalies in homocysteine metabolism have been implicated in disorders ranging from vascular disease to neural tube birth defects such as spina bifida. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BHMT2 Antibody (NBP1-85826) (0)

There are no publications for BHMT2 Antibody (NBP1-85826).
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WB Video Protocol

FAQs for BHMT2 Antibody (NBP1-85826). (Showing 1 - 1 of 1 FAQ).

  1. I was looking at the BHMT2 antibody IHC image in brain tissue and I want to know where is this protein expressed? Cytosolic or nuclear ? the image you are showing does not have details.
    • BHMT2 protein is not a very well published target and I do not see any research paper with its immuno-staining data. However, our antibodies NBP1-85826 and NBP2-15585 depicted a strong cytoplasmic staining with mild nuclear positivity for this target in IHC-P and ICC-IF assays. Human Protein Atlas also shows mainly cytoplasmic staining in hepatocytes, renal tubules, thyroid gland and a subset of cells in exocrine pancreas. Chadwick et al. (Genomics. 2000 Nov 15;70(1):66-73) reported that BHMT2 is expressed in liver and kidney, and at reduced levels in the brain, heart, and skeletal muscle.
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Bioinformatics

Gene Symbol BHMT2