PSMA/FOLH1/NAALADase I Antibody (GCP-04) - BSA Free

This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Rat, Canine.

We have publications tested in 5 applications: ICC/IF, IF/IHC, IHC, IHC-P, WB.

Showing Publications 1 - 10 of 10.

Publications using NBP1-45057	Applications	Species
Berry MR, Fadl-Alla BA, Samuelson J et al. Investigating PSMA differential expression in canine uroepithelial carcinomas to aid disease-based stratification and guide therapeutic selection BMC veterinary research 2022-12-20 [PMID: 36539731] (IHC, WB, ICC/IF, Canine, Human)	IHC, WB, ICC/IF	Canine, Human
Stopa B, Crowley J, JuhAsz C et al. Prostate-Specific Membrane Antigen as Target for Neuroimaging of Central Nervous System Tumors Molecular Imaging 2022-04-15 [PMID: 35517711]
Dowling M, Samuelson J, Fadl-Alla B et al. Overexpression of prostate specific membrane antigen by canine hemangiosarcoma cells provides opportunity for the molecular detection of disease burdens within hemorrhagic body cavity effusions. PLoS ONE 2019-01-02 [PMID: 30601866] (IHC-P, Human)	IHC-P	Human
Rovenska M, Hlouchova K, Sacha P et al. Tissue expression and enzymologic characterization of human prostate specific membrane antigen and its rat and pig orthologs. Prostate. 2008-02-01 [PMID: 18076021]
Sacha P, Zamecnik J, Barinka C et al. Expression of glutamate carboxypeptidase II in human brain. Neuroscience. 2007-02-01 [PMID: 17150306]
Barinka C, Mlcochova P, Sacha P et al. Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding. Eur J Biochem. 2004-07-01 [PMID: 15206943]
Barinka C, Sacha P, Sklenar J et al. Identification of the N-glycosylation sites on glutamate carboxypeptidase II necessary for proteolytic activity. Protein Sci. 2004-06-01 [PMID: 15152093]
Barinka C, Rinnova M, Sacha P et al. Substrate specificity, inhibition and enzymological analysis of recombinant human glutamate carboxypeptidase II. J Neurochem. 2002-02-01 [PMID: 11905994]
Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01
Oliveira D, Stegmayr C, Heinzel A et al. High uptake of 68Ga-PSMA and 18F-DCFPyL in the peritumoral area of rat gliomas due to activated astrocytes EJNMMI Res 2020-05-25 [PMID: 32451793] (IF/IHC, Rat)	IF/IHC	Rat

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Holly Pondenis	WB	Canine and Human	12/15/2017	View Summary Application Western Blot Sample Tested canine prostate carcinoma,Human prostate cancer cell line (PC3),LNCaP human prostate cancer cell line Species Canine and Human Lot 522819 Comments Comments Reduce denaturing, 4-20% gel 50ug of protein per lane except LNCaP (5ug) PVDF membrane Blocking: 5% Milk TBST, 1 hour room temperature Dilution 1:250, 4 C overnight Secondary: rabbit anti-mouse HRP Detection: ECL femto
Enlarge	5 reviewed by: Verified Customer	WB	Human	04/02/2015	View Summary Application Western Blot Sample Tested prostate cancer cell line Species Human

Array NBP1-45057

• PSMA/FOLH1/NAALADase I Antibodies
• PSMA/FOLH1/NAALADase I Lysates
• PSMA/FOLH1/NAALADase I Proteins
• PSMA/FOLH1/NAALADase I Proteins and Enzymes
• PSMA/FOLH1/NAALADase I ELISA