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CBS Antibody

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Genetic Strategies: Western Blot: CBS Antibody [NBP1-33518] - Wild-type (WT) and CBS knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody. ...read more
Genetic Strategies: Western Blot: CBS Antibody [NBP1-33518] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS ...read more
Western Blot: CBS Antibody [NBP1-33518] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to ...read more
Immunohistochemistry-Paraffin: CBS Antibody [NBP1-33518] - OVCAR3 xenograft , using CBS antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: CBS Antibody [NBP1-33518] - Mouse tissue extracts (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody ...read more
Western Blot: CBS Antibody [NBP1-33518] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody diluted at 1:1000. ...read more
Immunoprecipitation: CBS Antibody [NBP1-33518] - CBS antibody immunoprecipitates CBS protein in IP experiments. IP samples: HepG2 whole cell extract. A. 50 ug HepG2 whole cell extract. B. Control with 4 ug of preimmune ...read more
Immunohistochemistry-Paraffin: CBS Antibody [NBP1-33518] - CBS antibody detects CBS protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.CBS stained by CBS antibody ...read more
Immunocytochemistry/ Immunofluorescence: CBS Antibody [NBP1-33518] - CBS antibody detects CBS protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IP, KD, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

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CBS Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human CBS. The exact sequence is proprietary.
Localization
Cytoplasm, Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CBS
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Knockdown Validated
  • Knockout Validated
  • Western Blot 1:500-1:3000
Theoretical MW
61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for CBS Antibody

  • cystathionine-beta-synthase
  • EC 4.2.1.2210Beta-thionase

Background

The protein encoded by this gene is involved in the transsulfuration pathway. The first step of this pathway, from homocysteine to cystathionine, is catalyzed by this protein. CBS deficiency can cause homocystinuria which affects many organs and tissues, including the eyes and the skeletal, vascular and central nervous systems.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CBS Antibody (NBP1-33518) (0)

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Secondary Antibodies

 

Isotype Controls

Additional CBS Products

Research Areas for CBS Antibody (NBP1-33518)

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Bioinformatics

Gene Symbol CBS