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ENOSF1 Antibody

Images

 
Western Blot: ENOSF1 Antibody [NBP2-13962] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). ...read more
Orthogonal Strategies: Immunohistochemistry-Paraffin: ENOSF1 Antibody [NBP2-13962] - Staining in human kidney and ovary tissues using anti-ENOSF1 antibody. Corresponding ENOSF1 RNA-seq data are presented for the ...read more
Immunohistochemistry-Paraffin: ENOSF1 Antibody [NBP2-13962] - Staining of human kidney shows high expression.
Immunohistochemistry-Paraffin: ENOSF1 Antibody [NBP2-13962] - Staining of human ovary shows low expression as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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ENOSF1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: QHLIIFDYISVSASLENRVCEYVDHLHEHFKYPVMIQRASYMPPKDPGYSTEMKEESVKKHQYPDGEVWKK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ENOSF1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
ENOSF1 Protein (NBP2-13962PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ENOSF1 Antibody

  • Antisense RNA to thymidylate synthase
  • EC 5.-
  • enolase superfamily member 1
  • HSRTSBETA
  • rTS beta
  • rTS
  • RTSrTS alpha
  • TYMSASmitochondrial enolase superfamily member 1

Background

The rTS gene codes for a naturally occurring antisense RNA to thymidylate synthase (TS) mRNA and two proteins (rTSalpha and rTSbeta (also known as ENOSF1)). The role of the major protein product of rTS, ENOSF1 has been linked to alterations in TS protein expression, but the precise function of ENOSF1 is unknown. Expression of rTS is associated with growth arrest in cell culture, but its overexpression has been noted in cells resistant to anticancer drugs such as 5-fluorouracil and methotrexate. Studies have shown that increased expression of ENOSF1 is associated with the decrease in TS protein expression due to production of novel, diffusible signal molecules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ENOSF1