![Western Blot: BHMT Antibody [NBP1-00161] - Rat Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/BHMT-Antibody-Western-Blot-NBP1-00161-img0003.jpg "Western Blot: BHMT Antibody [NBP1-00161] - Rat Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
| Reactivity | Hu, Mu, Rt, CaSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-EQQLKELFEKQK corresponding to C-Terminus according to NP_001704.1 . |
| Predicted Species | Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | BHMT |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Approx 45 kDa band observed in Rat Liver lysates (calculated MW of 44.9 kDa according to human NP_001704.1, 45.0 kDa according to mouse NP_057877.1 and rat NP_110477.1). This product has been successfully used in WB on Mouse (PMID:28605831, 23807810 and 22209966). In transfected HEK293 transiently expressing BHMT a band of approx. 49kDa is observed. This band is not observed in the non-transfected HEK293. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for BHMT Antibody (NBP1-00161)Find related products by research area.
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| Gene Symbol | BHMT |
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| Uniprot |
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![Immunohistochemistry-Paraffin: BHMT Antibody [NBP1-00161] - Human Kidney tissue. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/BHMT-Antibody-Immunohistochemistry-Paraffin-NBP1-00161-img0005.jpg "Immunohistochemistry-Paraffin: BHMT Antibody [NBP1-00161] - Human Kidney tissue. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Western Blot: BHMT Antibody [NBP1-00161] - HEK293 overexpressing BHMT and probed with this antibody (mock transfection in first lane).](http://images.novusbio.com/fullsize/BHMT-Antibody-Western-Blot-NBP1-00161-img0001.jpg "Western Blot: BHMT Antibody [NBP1-00161] - HEK293 overexpressing BHMT and probed with this antibody (mock transfection in first lane).")
![Immunohistochemistry-Paraffin: BHMT Antibody [NBP1-00161] - Human Liver tissue. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/BHMT-Antibody-Immunohistochemistry-Paraffin-NBP1-00161-img0004.jpg "Immunohistochemistry-Paraffin: BHMT Antibody [NBP1-00161] - Human Liver tissue. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
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