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BHMT Antibody

Images

 
Independent Antibodies: Western Blot: BHMT Antibody [NBP2-38850] - Analysis using Anti-BHMT antibody NBP2-38850 (A) shows similar pattern to independent antibody NBP1-88611 (B).
Immunohistochemistry-Paraffin: BHMT Antibody [NBP2-38850] - Staining of human cerebral cortex.
Western Blot: BHMT Antibody [NBP2-38850] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue
Immunohistochemistry-Paraffin: BHMT Antibody [NBP2-38850] - Staining of human kidney shows high expression.
Immunohistochemistry-Paraffin: BHMT Antibody [NBP2-38850] - Staining of human lymph node shows low expression as expected.
Orthogonal Strategies: Immunohistochemistry-Paraffin: BHMT Antibody [NBP2-38850] - Staining in human kidney and lymph node tissues using anti-BHMT antibody. Corresponding BHMT RNA-seq data are presented for the ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: BHMT Antibody [NBP2-38850] - Staining of human cerebral cortex, colon, kidney and testis using Anti-BHMT antibody NBP2-38850 (A) shows similar protein ...read more
Immunohistochemistry-Paraffin: BHMT Antibody [NBP2-38850] - Staining of human colon.
Immunohistochemistry-Paraffin: BHMT Antibody [NBP2-38850] - Staining of human testis.
Immunohistochemistry-Paraffin: BHMT Antibody [NBP2-38850] - Staining of human kidney using Anti-BHMT antibody NBP2-38850.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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BHMT Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: KEYWENLRIASGRPYNPSMSKPDGWGVTKGTAELMQQKEATTEQQLKELFE
Predicted Species
Mouse (90%), Rat (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BHMT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
BHMT Protein (NBP2-38850PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for BHMT Antibody

  • betaine homocysteine methyltransferase
  • betaine-homocysteine methyltransferase
  • betaine--homocysteine S-methyltransferase 1
  • betaine--homocysteine S-methyltransferase
  • BHMT1
  • EC 2.1.1
  • EC 2.1.1.5

Background

BHMT encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for BHMT Antibody (NBP2-38850) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BHMT
Uniprot