![Western Blot: BHMT Antibody [NBP1-55288] - Lanes: Lane1: 20 ug rat liver lysate Primary Antibody Dilution: 1:2000 Secondary Antibody: Anti-rabbit HRP Secondary Antibody Dilution: 1:15000 Gene Name: BHMT Submitted by: Anonymous](http://images.novusbio.com/fullsize/BHMT-Antibody-Western-Blot-NBP1-55288-img0007.jpg "Western Blot: BHMT Antibody [NBP1-55288] - Lanes: Lane1: 20 ug rat liver lysate Primary Antibody Dilution: 1:2000 Secondary Antibody: Anti-rabbit HRP Secondary Antibody Dilution: 1:15000 Gene Name: BHMT Submitted by: Anonymous")
| Reactivity | Hu, Rt, FiSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Description | The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution. |
| Immunogen | Synthetic peptides corresponding to BHMT(betaine-homocysteine methyltransferase) The peptide sequence was selected from the N terminal of BHMT.
Peptide sequence AVEHPEAVRQLHREFLRAGSNVMQTFTFYASEDKLENRGNYVLEKISGQE. The peptide sequence for this immunogen was taken from within the described region. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | BHMT |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 45 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, 2% Sucrose |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
| Publication using NBP1-55288 | Applications | Species |
|---|---|---|
| Lescat L, VEron V, Mourot B et al. Chaperone-Mediated Autophagy in the light of evolution: insight from fish Mol. Biol. Evol. 2020-05-21 [PMID: 32437540] (Fish) | Fish |
Secondary Antibodies |
Isotype Controls |
Research Areas for BHMT Antibody (NBP1-55288)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry-Paraffin: BHMT Antibody [NBP1-55288] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/BHMT-Antibody-Immunohistochemistry-Paraffin-NBP1-55288-img0003.jpg "Immunohistochemistry-Paraffin: BHMT Antibody [NBP1-55288] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.")
![Western Blot: BHMT Antibody [NBP1-55288] - Analysis of 293T cell lysate. Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/BHMT-Antibody-Western-Blot-NBP1-55288-img0004.jpg "Western Blot: BHMT Antibody [NBP1-55288] - Analysis of 293T cell lysate. Antibody Dilution: 1.0 ug/ml.")
![Western Blot: BHMT Antibody [NBP1-55288] - Lanes: Lane1 : 20 ug rat liver lysate Primary Antibody Dilution: 1 : 1000 Secondary Antibody: Anti-rabbit HRP Secondary Antibody Dilution: 1 : 15000 Gene name: BHMT Submitted by: Anonymous.](http://images.novusbio.com/fullsize/BHMT-Antibody-Western-Blot-NBP1-55288-img0005.jpg "Western Blot: BHMT Antibody [NBP1-55288] - Lanes: Lane1 : 20 ug rat liver lysate Primary Antibody Dilution: 1 : 1000 Secondary Antibody: Anti-rabbit HRP Secondary Antibody Dilution: 1 : 15000 Gene name: BHMT Submitted by: Anonymous.")
![Western Blot: BHMT Antibody [NBP1-55288] - Reccomended Titration: 2.5 ug/ml Positive Control: Human Fetal Liver cell lysate](http://images.novusbio.com/fullsize/BHMT-Antibody-Western-Blot-NBP1-55288-img0006.jpg "Western Blot: BHMT Antibody [NBP1-55288] - Reccomended Titration: 2.5 ug/ml Positive Control: Human Fetal Liver cell lysate")
![Immunocytochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg)
![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")