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Bcl-2 related protein A1 Antibody [Janelia Fluor® 549]

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Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Janelia Fluor 549

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-76715AF350
Alexa Fluor 405
NBP1-76715AF405
Alexa Fluor 488
NBP1-76715AF488
Alexa Fluor 532
NBP1-76715AF532
Alexa Fluor 594
NBP1-76715AF594
Alexa Fluor 647
NBP1-76715AF647
Alexa Fluor 700
NBP1-76715AF700
Alexa Fluor 750
NBP1-76715AF750
Biotin
NBP1-76715B
CoraFluor 1
NBP1-76715CL1
DyLight 350
NBP1-76715UV
DyLight 405
NBP1-76715V
DyLight 488
NBP1-76715G
DyLight 550
NBP1-76715R
DyLight 594
NBP1-76715DL594
DyLight 650
NBP1-76715C
DyLight 680
NBP1-76715FR
DyLight 755
NBP1-76715IR
FITC
NBP1-76715F
HRP
NBP1-76715H
Janelia Fluor 525
NBP1-76715JF525
Janelia Fluor 585
NBP1-76715JF585
Janelia Fluor 635
NBP1-76715JF635
Janelia Fluor 646
NBP1-76715JF646
Janelia Fluor 669
NBP1-76715JF669
PerCP
NBP1-76715PCP
Unconjugated
NBP1-76715
mFluor Violet 450 SE
NBP1-76715MFV450
mFluor Violet 500 SE
NBP1-76715MFV500
mFluor Violet 610 SE
NBP1-76715MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-76715
BSA Free
NBP1-76715
BSA Free
NBP1-76715-0.025mg
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Bcl-2 related protein A1 Antibody [Janelia Fluor® 549] Summary

Immunogen
Antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Bfl-1. The immunogen is located within the last 50 amino acids of Bfl-1. Amino Acid Squence: ENGFVKKFEPKSG
Specificity
At least two isoforms of Bfl-1 are known to exist; this antibody will only detect the larger isoform.
Predicted Species
Bovine (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BCL2A1
Purity
Peptide affinity purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

0

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Peptide affinity purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Bcl-2 related protein A1 Antibody [Janelia Fluor® 549]

  • ACC-1
  • ACC-2
  • Bcl-2 related protein A1
  • BCL2A1
  • Bcl2L5
  • Bcl2-L-5
  • BCL2L5HBPA1
  • Bcl-2-like protein 5
  • BCL2-related protein A1
  • Bfl-1
  • BFL1bcl-2-related protein A1
  • GRS
  • GRSbcl2-L-5
  • hematopoietic BCL2-related protein A1
  • Hemopoietic-specific early response protein
  • Protein BFL-1
  • Protein GRS

Background

Bcl-2 related protein A1; also known as Bcl2A1 and Bfl1, is an anti-apoptotic Bcl-2 family member preferentially expressed in hematopoietic and endothelial cells (1). Bfl1 is involved in a variety of cellular activities such as embryonic development, homeostatis and tumorigenesis. Expression of Bfl1 is stimulated by NF-kB in response to inflammatory stimuli and is up-regulated by TNF-alpha, IL1-beta, IGF1, GMC-SF, CD40 and phorbol ester (2-3). Blf1 has also been shown to reduce the release of pro-apoptotic cytochrome c from the mitochondria, block caspase activation and suppress apoptosis induced by p53 (4-5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol BCL2A1