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Bcl-2 related protein A1 Antibody [CoraFluor™ 1]

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Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-76715AF350
Alexa Fluor 405
NBP1-76715AF405
Alexa Fluor 488
NBP1-76715AF488
Alexa Fluor 532
NBP1-76715AF532
Alexa Fluor 594
NBP1-76715AF594
Alexa Fluor 647
NBP1-76715AF647
Alexa Fluor 700
NBP1-76715AF700
Alexa Fluor 750
NBP1-76715AF750
Biotin
NBP1-76715B
DyLight 350
NBP1-76715UV
DyLight 405
NBP1-76715V
DyLight 488
NBP1-76715G
DyLight 550
NBP1-76715R
DyLight 594
NBP1-76715DL594
DyLight 650
NBP1-76715C
DyLight 680
NBP1-76715FR
DyLight 755
NBP1-76715IR
FITC
NBP1-76715F
HRP
NBP1-76715H
Janelia Fluor 525
NBP1-76715JF525
Janelia Fluor 549
NBP1-76715JF549
Janelia Fluor 585
NBP1-76715JF585
Janelia Fluor 635
NBP1-76715JF635
Janelia Fluor 646
NBP1-76715JF646
Janelia Fluor 669
NBP1-76715JF669
PerCP
NBP1-76715PCP
Unconjugated
NBP1-76715
mFluor Violet 450 SE
NBP1-76715MFV450
mFluor Violet 500 SE
NBP1-76715MFV500
mFluor Violet 610 SE
NBP1-76715MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-76715
BSA Free
NBP1-76715
BSA Free
NBP1-76715-0.025mg
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Bcl-2 related protein A1 Antibody [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Bfl-1. The immunogen is located within the last 50 amino acids of Bfl-1. Amino Acid Squence: ENGFVKKFEPKSG
Specificity
At least two isoforms of Bfl-1 are known to exist; this antibody will only detect the larger isoform.
Predicted Species
Bovine (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BCL2A1
Purity
Peptide affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Peptide affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Bcl-2 related protein A1 Antibody [CoraFluor™ 1]

  • ACC-1
  • ACC-2
  • Bcl-2 related protein A1
  • BCL2A1
  • Bcl2L5
  • Bcl2-L-5
  • BCL2L5HBPA1
  • Bcl-2-like protein 5
  • BCL2-related protein A1
  • Bfl-1
  • BFL1bcl-2-related protein A1
  • GRS
  • GRSbcl2-L-5
  • hematopoietic BCL2-related protein A1
  • Hemopoietic-specific early response protein
  • Protein BFL-1
  • Protein GRS

Background

Bcl-2 related protein A1; also known as Bcl2A1 and Bfl1, is an anti-apoptotic Bcl-2 family member preferentially expressed in hematopoietic and endothelial cells (1). Bfl1 is involved in a variety of cellular activities such as embryonic development, homeostatis and tumorigenesis. Expression of Bfl1 is stimulated by NF-kB in response to inflammatory stimuli and is up-regulated by TNF-alpha, IL1-beta, IGF1, GMC-SF, CD40 and phorbol ester (2-3). Blf1 has also been shown to reduce the release of pro-apoptotic cytochrome c from the mitochondria, block caspase activation and suppress apoptosis induced by p53 (4-5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol BCL2A1